A5076396754- Mesenchymal stem cell research
- Cancer Research and Treatments
- Virus-based gene therapy research
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- Enzyme function and inhibition
- Autophagy in Disease and Therapy
- Cancer, Hypoxia, and Metabolism
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Extracellular vesicles in disease
- CAR-T cell therapy research
- Peptidase Inhibition and Analysis
- MicroRNA in disease regulation
- Photosynthetic Processes and Mechanisms
- 3D Printing in Biomedical Research
- Immune cells in cancer
- Genetic factors in colorectal cancer
- Pancreatic and Hepatic Oncology Research
- Dendrimers and Hyperbranched Polymers
- Molecular Biology Techniques and Applications
- Lipid metabolism and biosynthesis
- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Polyamine Metabolism and Applications
Biomedical Research Center of the Slovak Academy of Sciences
2018-2024
Cancer Research Institute of the Slovak Academy of Sciences
2008-2024
Molecular Oncology (United States)
2016-2024
Slovak Academy of Sciences
2007-2017
Comenius University Bratislava
2004
Abstract Human adipose tissue–derived mesenchymal stem cells (AT-MSC) are considered to be a promising source of autologous in personalized cell-based therapies. Tumor tracking properties MSC provide an attractive opportunity for targeted transgene delivery into the sites tumor formation. In present study, we addressed whether suicide gene introduction human AT-MSC could produce tumor-specific prodrug converting cellular vehicle chemotherapy. We prepared yeast fusion cytosine...
Abstract Background Previously, we validated capability of human adipose tissue‐derived mesenchymal stem cells (AT‐MSC) to serve as cellular vehicles for gene‐directed enzyme prodrug molecular chemotherapy. Yeast fusion cytosine deaminase : uracil phosphoribosyltransferase expressing AT‐MSC (CD y ‐AT‐MSC) combined with systemic 5‐fluorocytosine (5FC) significantly inhibited growth colon cancer xenografts. We aimed determine the cytotoxic efficiency other tumour both in vitro and vivo ....
Cells of the tumor microenvironment are recognized as important determinants biology. The adjacent non-malignant cells can regulate drug responses cancer by secreted paracrine factors and direct interactions with cells. Human mesenchymal stromal (MSC) actively contribute to microenvironment. Here we focused on their response chemotherapy during treatment these become affected. We have shown that secretory phenotype behavior influenced cisplatin differs from naïve MSC. MSC were more resistant...
Metastatic spread of tumor cells remains a serious problem in cancer treatment. Gene-directed enzyme/prodrug therapy mediated by tumor-homing genetically engineered mesenchymal stromal (MSC) represents promising therapeutic modality for elimination disseminated cells. Efficacy gene-directed can be improved combination individual systems. We aimed to define the effect two systems gene MSC, and evaluate ability systemically administered inhibit growth experimental metastases derived from human...
Background Mesenchymal stromal cells (MSC) are a promising tool for targeted cancer therapy due to their tumour-homing ability. Intrinsic resistance enables the MSC longer tolerate therapeutic factors, such as prodrug converting enzymes, cytokines and pro-apoptotic proteins. Tumour necrosis factor alpha (TNFα) is known be cytotoxic variety of exert tumour-destructive capacity. Methods were retrovirally transduced stable express an exogenous gene encoding desired agent hTNFα. The effect...
We report on a simple iron oxide (Venofer) labeling procedure of dental pulp mesenchymal stem cells (DP-MSCs) and DP-MSCs transduced with yeast cytosinedeaminase::uracilphosphoribosyltransferase (yCD::UPRT-DP-MSCs). Venofer is drug approved for intravenous application to treat deficiency anemia in patients. did not affect or yCD::UPRT-DP-MSCs viability growth kinetics. Electron microscopy labeled showed internalized nanoparticles endosomes. MRI relativity measurement phantom arrangement...
Tumor necrosis factor alpha (TNFα) is a pleiotropic cytokine with both anti-tumorigenic and pro-tumorigenic activity, affecting tumor cell biology, the balance between survival death. The final effect of TNFα dependent on type malignant cells, potential to arrest cancer progression.In order explain diverse cellular response TNFα, we engineered melanoma colorectal carcinoma lines stably overexpressing this cytokine.Under overexpression, significant upregulation two genes was observed:...
Mesenchymal stromal cells (MSCs) are considered to be suitable vehicles for cellular therapy in various conditions. The expression of reporter and/or effector protein(s) enabled both the identification MSCs within organism and exploitation targeted tumor therapies. aim this study was evaluate changes induced by retrovirus-mediated transgene vitro. Human Adipose Tissue-derived (AT-MSCs) were transduced express (i) enhanced green fluorescent protein (EGFP) transgene, (ii) fusion yeast cytosine...
Decitabine (DAC), a DNA methyltransferase (DNMT) inhibitor, is tested in combination with conventional anticancer drugs as treatment option for various solid tumors. Although epigenome modulation provides promising avenue treating resistant cancer types, more studies are required to evaluate its safety and ability normalize the aberrant transcriptional profiles. As deoxycytidine kinase (DCK)-mediated phosphorylation rate-limiting step DAC metabolic activation, we hypothesized that...
Mesenchymal stromal cells (MSC) exhibit beneficial properties to serve as cellular vehicles for enzyme/prodrug cancer gene therapy approaches. We have previously shown that engineered human adipose tissue-derived MSC in combination with non-toxic prodrug mediated substantial cytotoxic and antitumor effect. The aim of this study was develop advanced 3D cultivation method modelling the therapeutic outcome vitro. used expressing fusion transgene cytosine...
Abstract We report results showing that the silencing of carbonic anhydrase I (siCA1) in prostatic (PC3) tumour cells has a significant impact on exosome formation. An increased diameter, concentration and diversity produced exosomes were noticed as consequence this knock‐down. The protein composition exosomes' cargo was also altered. Liquid chromatography mass spectrometry analyses identified 42 proteins significantly altered PC3 siCA1 compared with controls. affected are mainly involved...
Cell-based anticancer therapy using mesenchymal stromal cells (MSCs) engineered to express therapeutic genes has a potential target the cancer in vivo. Metastatic dissemination of melanoma remains serious problem treatment. In our previous work we used MSCs overexpressing gene for tumor necrosis factor α (TNFα; MSCs/TNFα), and achieved inhibition xenograft growth when MCSs/TNFα were coinjected with subcutaneously. The TNFα as pleiotropic cytokine induces apoptosis cells, creates "tumor...
Spontaneous tumour regression after high-dose therapy and autologous stem cell transplantation is associated with the aplastic anaemia-like syndrome presence of polyclonal autoantibodies against carbonic anhydrase I (CA I). When cells were grown in vitro patients' sera positive for anti-CA autoantibodies, their morphological pattern was altered. These changes accompanied by modifications gene expression profile. We observed downregulation genes basal lamina assembly (collagen type IV alpha...
Abstract We report the silencing of CA1 mRNA in PC3 and MDA cells. The levels coding protein knock‐down (CA1 siRNA) cells have been measured by RT‐PCR were approximately 5% (PC3) 20% (MDA‐MB‐231), respectively, level control (Mock used during silencing. In MDA‐MB‐231 cells, mRNAs for COL1A1 COL4A4 up‐regulated. CTHRC1, LAMC2, WNT7B not changed when compared to control. morphology treatments remained same. On Western blots, lysate from silenced showed lower CA I as well.
The phosphatidylglycerolphosphate synthase (CDP-diacylglycerol:sn-glycerol-3-phosphate 3-phosphatidyltransferase, EC 2.7.8.5) is an essential enzyme in biosynthesis of cardiolipin. In this work we report the isolation, heterological cloning, molecular characterization and physical mapping Saccharomyces cerevisiae PEL1/PGS1 homologue from Kluyveromyces lactis. pel1 mutant strain S. was used to isolate by screening a K. lactis genomic library. novel cloned gene named KlPGS1. Its coding region...
Cancer is one of the leading causes death worldwide.We still do not understand all details carcinogenesis, and effective treatment lacking for many oncological diseases.Animal models provide an irreplaceable tool to observe growth spreading neoplastic cells in environment living organisms, test efficacy cancer treatment, side effects, toxicity, study tumor microenvironment.Mice are most often used model organisms because their easy handling, short reproductive period, multiple strains,...
ABSTRACT A promising approach to treat colorectal cancer (CRC) involves combining chemotherapy, epigenetics, and gene therapy combat drug resistance. Multifunctional nanocarriers have emerged as a valuable tool for targeted CRC therapy. By delivering multiple treatments directly cells, these offer the potential improved outcomes reduced side effects. PAMAM‐based dendrimers were functionalized with unique combination of folic acid, 5‐FU, SAHA, plasmid DNA pCIneoGFP delivery cells. Biophysical...
Colorectal cancer mortality is one of the most common cause cancer-related mortality.A multiple risk factors are associated with colorectal cancer, including hereditary, enviromental and inflammatory syndromes affecting gastrointestinal tract.Familial adenomatous polyposis (FAP) characterized by emergence hundreds to thousands polyps FAP syndrome caused mutations within coli (APC) tumor suppressor gene.We analyzed 21 rectal bacterial subclones isolated from patient 41-1 confirmed 5bp ACAAA...