A5032739598- Prenatal Screening and Diagnostics
- Cancer Genomics and Diagnostics
- Gestational Trophoblastic Disease Studies
- Genetic factors in colorectal cancer
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Endometrial and Cervical Cancer Treatments
- Cancer, Lipids, and Metabolism
- RNA modifications and cancer
- Mesenchymal stem cell research
- RNA Interference and Gene Delivery
- Epigenetics and DNA Methylation
- Ovarian cancer diagnosis and treatment
- Colorectal Cancer Treatments and Studies
- Plant biochemistry and biosynthesis
- Advanced Glycation End Products research
- Mitochondrial Function and Pathology
- Pregnancy and preeclampsia studies
- Cancer-related gene regulation
- Prostate Cancer Treatment and Research
- Plasma Applications and Diagnostics
- Genomics and Rare Diseases
- COVID-19 Clinical Research Studies
- Acute Myeloid Leukemia Research
- Cervical Cancer and HPV Research
Comenius University Bratislava
2015-2025
University Hospital Bratislava
2013-2023
Institute of Medical Biology
2009-2023
Medirex Group Academy
2022-2023
Martin University Hospital
2020
Slovak Medical University
2020
Alpha Medical (Slovakia)
2014
Vysoká Škola Zdravotníctva a Sociálnej Práce sv. Alžbety
2014
Slovak Academy of Sciences
2014
Cancer Research Institute of the Slovak Academy of Sciences
2014
<h3>Background:</h3> <i>NLRP7</i> (NALP7) has recently been identified as the causative gene for familial recurrent hydatidiform mole (FRHM), a rare autosomal recessive condition in which affected women have molar pregnancies of diploid biparental origin. To date only small number families described. Our objectives were to investigate diversity mutations and their localisation one or both isoforms NLRP7, by screening large series with FRHM examine normal expression NLRP7 ovarian tissue....
Abstract: Magnetic hyperthermia, or the heating of tissues using magnetic materials, is a promising approach for treating cancer. We found that human mesenchymal stem cells (MSCs) isolated from various and MSCs expressing yeast cytosine deaminase::uracil phosphoribosyl transferase suicide fusion gene ( yCD::UPRT ) can be labeled with Venofer, an iron oxide carbohydrate nanoparticle. Venofer labeling did not affect cell proliferation ability to home tumors. All Venofer-labeled released...
Extracellular vesicles (EVs) are nowadays a target of interest in cancer therapy as successful drug delivering tool. Based on their many beneficial biocompatible properties designed to transport nucleic acids, proteins, various nanomaterials or chemotherapeutics. derived from mesenchymal stem/stromal cells (MSCs) possess tumor-homing abilities. This inspired us engineer the MSC's EVs be packed with chemotherapeutic agents and deliver it Trojan horse directly into tumor cells. In our study,...
The natural behavior of mesenchymal stem cells (MSCs) and their exosomes in targeting tumors is a promising approach for curative therapy. Human tumor tropic isolated from various tissues MSCs engineered to express the yeast cytosine deaminase::uracil phosphoribosyl transferase suicide fusion gene (yCD::UPRT-MSCs) released conditional medium (CM). Exosomes all tissue specific yCD::UPRT-MSCs contained mRNA exosome's cargo. When CM was applied cells, were internalized by recipient presence...
Breast carcinoma is the most common cancer with high mortality caused by metastatic disease.New molecular biomarkers predicting tumour's potential would therefore improve metastasis prevention and personalised care.The aim of study was to investigate relationship between DNA methylation levels in invasivity metastasising associated genes aberrant protein expression also evaluate whether a similar level present tumour circulating cell-free for utilising plasma as prognostic biomarker.By using...
Methylation of the cytosine residues within CpG dinucleotides plays an important role in fundamental cellular processes, human diseases and even cancer.The DNA methylation represents a very stable sign therefore may be used as valuable marker for cancer screening.Epigenetic biomarkers are independent classical morphology thus show extensive potential to overcome limitations cytology.Several epigenetic markers have been reported detectable body fluids such bronchial aspirate, sputum, plasma...
This short communication demonstrates how tandem repeat genotyping can identify the origin of gestational choriocarcinoma. The choriocarcinoma in our three cases was determined using repeats technique, which involved quantitative fluorescent PCR and fragmentation analysis. In Case 1 despite no medical history molar pregnancy, DNA analysis indicated that originated from a homozygous complete hydatidiform mole. We conclude, patient's abortion 10 years prior to diagnosis an undiagnosed 2 3...
We report on a simple iron oxide (Venofer) labeling procedure of dental pulp mesenchymal stem cells (DP-MSCs) and DP-MSCs transduced with yeast cytosinedeaminase::uracilphosphoribosyltransferase (yCD::UPRT-DP-MSCs). Venofer is drug approved for intravenous application to treat deficiency anemia in patients. did not affect or yCD::UPRT-DP-MSCs viability growth kinetics. Electron microscopy labeled showed internalized nanoparticles endosomes. MRI relativity measurement phantom arrangement...
Recently, we reported about exosomes possessing messenger RNA (mRNA) of suicide gene secreted from mesenchymal stem/stromal cells (MSCs) engineered to express the gene-fused yeast cytosine deaminase::uracil phosphoribosyltransferase (yCD::UPRT). The yCD::UPRT-MSC are internalized by tumor and intracellularly convert prodrug 5-fluorocytosine (5-FC) cytotoxic drug 5-fluorouracil (5-FU). Human with potential metastasize release involved in creation a premetastatic niche at predicted organs. We...
Plasma medicine is a new field focusing on biomedical and clinical applications of cold gas plasmas, including their anticancer effects. Cold plasmas can be applied directly or indirectly as plasma-activated liquids (PAL). The effects cell growth medium (PAM) phosphate buffered saline (PAPBS) were tested, using plasma pen generating streamer corona discharge in ambient air, different cancer lines (melanoma A375, glioblastoma LN229 pancreatic MiaPaCa-2) normal cells (human dermal fibroblasts...
In current clinical practice, effective cancer testing and screening paradigms are limited to specific types of cancer, exhibiting varying efficiency, acceptance, adherence. Cell-free DNA (cfDNA) methylation profiling holds promise in providing information about the presence malignity regardless its type location while leveraging blood-based liquid biopsies as a method obtain analytical samples. However, technical difficulties, costs challenges resulting from biological variations, tumor...
Abstract Malignant germ cell tumors (GCTs) are characterized by pluripotency and exhibit diverse embryonic extraembryonic structures. Approximately 10% of malignant GCTs mixed tumors. The precise histogenic origins remain elusive, mostly because their rare occurrence. aim this study is to determine the origin an extremely tumor ovary consisting embryonal carcinoma non-gestational choriocarcinoma components using short tandem repeats genotyping quantitative-fluorescence PCR capillary...
Endometrial cancer is a significant public health concern with rising incidence rates globally. Understanding the molecular mechanisms underlying this disease crucial for developing effective therapeutic strategies. Our study aimed to characterize transcriptional changes in endometrial tissues compared adjusted healthy tissue. Using RNA sequencing, we identified 2483 differentially expressed genes (DEGs), including protein-coding genes, long non-coding RNAs (lncRNAs), and microRNAs (miRNAs)....
Abstract In this study, we aimed to elucidate the role of gut microbiome in multistage progression colorectal cancer (CRC). We analyse bacterial composition three clinical groups: healthy individuals, patients with precancerous polyps (adenomas), and cancerous tumours (carcinomas). Biopsy samples were profiled using Illumina MiSeq platform. Significant differences microbiota emerged between groups. The tumour-associated showed increased diversity specific enrichments, including...
Statin treatment of hypercholesterolemia is accompanied also with depletion the mevalonate intermediates, including farnesyl pyrophosphate (FPP) and geranylgeranyl (GGPP) necessary for proper function small GTPases. These include Ras proteins, prevalently mutated in pancreatic cancer. In our study, we evaluated effect three key intermediates pathway on GFP-K-Ras protein localization gene expression profile cancer cells after exposure to individual statins. effects were tested MiaPaCa-2 human...
Abstract This study investigated germline and somatic mutations in endometrial precancerous conditions carcinoma to elucidate the molecular landscape of cancer development. Germline pathogenic variants were identified 30.14% cohort patients (n = 205). The most frequently altered genes CHEK2 , SOS1 NBN SLX4 . In 39), 20.51% had PVs. Somatic mutation analysis tumor tissues revealed frequent PTEN PIK3CA ARID1A TP53 Notably, mean age with did not significantly differ from those without, either...
The Y chromosome accumulates male-related genes including sex-determining region of Y-chromosome (SRY) and several spermatogenesis-related genes. long arm contains azoospermia factor (AZF) (including sub-regions AZFa, AZFb AZFc). Microdeletions in this are responsible for oligospermia result the male infertility. aim study was to analyze incidence microdeletions AZF patients with from Slovakia.Over period 2005 2009 a total 239 men (mean age 31.74 years) were analyzed. diagnosis established...
Fighting resistance to antibiotics and chemotherapeutics has brought bioactive peptides the fore. Peptaibols are short α-aminoisobutyric acid-containing produced by Trichoderma species. Here, we studied production of peptaibols atroviride O1 evaluated their antibacterial anticancer activity against drug-sensitive multidrug-resistant bacterium cancer cell lines. This was substantiated an analysis peptaibol synthetase-encoding gene. Atroviridins, 20-residue were detected using MALDI-TOF mass...