A5101750454- Cervical Cancer and HPV Research
- Prenatal Screening and Diagnostics
- Gestational Trophoblastic Disease Studies
- Ovarian cancer diagnosis and treatment
- Endometrial and Cervical Cancer Treatments
- Polyomavirus and related diseases
- PI3K/AKT/mTOR signaling in cancer
- Cancer-related Molecular Pathways
- Erythrocyte Function and Pathophysiology
- Bioinformatics and Genomic Networks
- GDF15 and Related Biomarkers
- Circular RNAs in diseases
- Testicular diseases and treatments
- Advanced biosensing and bioanalysis techniques
- Neuroendocrine Tumor Research Advances
- Neonatal Health and Biochemistry
- Congenital Anomalies and Fetal Surgery
- Sarcoma Diagnosis and Treatment
- Cytomegalovirus and herpesvirus research
- Hepatitis B Virus Studies
- Hedgehog Signaling Pathway Studies
- Genetic Syndromes and Imprinting
- Lung Cancer Research Studies
- Hemoglobin structure and function
- Social Media and Politics
Comenius University Bratislava
2022-2024
University Hospital Bratislava
2022-2024
Institute of Medical Biology
2022-2024
University of Debrecen
1991-2011
Hungarian Academy of Sciences
2006
The University of Texas Medical Branch at Galveston
1992
To determine the prevalence, type, physical state, and viral load of human papillomavirus (HPV) DNA in cases head neck cancer recurrent respiratory papillomatosis (RRP).The prevalence type HPV was determined 27 fresh frozen tissue specimens from patients with cancers 16 10 RRP by MY09/MY11 GP5+/GP6+ nested polymerase chain reaction (PCR) subsequent restriction enzyme cleavage. The state analysed E1, E2, E1E2 specific PCRs Southern blot hybridisation (SBH).HPV detected 13 papillomas. Both low...
This short communication demonstrates how tandem repeat genotyping can identify the origin of gestational choriocarcinoma. The choriocarcinoma in our three cases was determined using repeats technique, which involved quantitative fluorescent PCR and fragmentation analysis. In Case 1 despite no medical history molar pregnancy, DNA analysis indicated that originated from a homozygous complete hydatidiform mole. We conclude, patient's abortion 10 years prior to diagnosis an undiagnosed 2 3...
Background: Survivin, a novel member of the inhibitor apoptosis family, plays an important role in cell cycle regulation. A common polymorphism at survivin gene promoter (G/C position 31) was shown to be correlated with expression cancer lines. Aim: To investigate whether this could involved development human papillomavirus (HPV)-associated cervical carcinoma. Methods: Survivin detected patients cancer, equivocal cytological atypia and control population using polymerase chain reaction...
Abstract Malignant germ cell tumors (GCTs) are characterized by pluripotency and exhibit diverse embryonic extraembryonic structures. Approximately 10% of malignant GCTs mixed tumors. The precise histogenic origins remain elusive, mostly because their rare occurrence. aim this study is to determine the origin an extremely tumor ovary consisting embryonal carcinoma non-gestational choriocarcinoma components using short tandem repeats genotyping quantitative-fluorescence PCR capillary...
Abstract This study investigated germline and somatic mutations in endometrial precancerous conditions carcinoma to elucidate the molecular landscape of cancer development. Germline pathogenic variants were identified 30.14% cohort patients (n = 205). The most frequently altered genes CHEK2 , SOS1 NBN SLX4 . In 39), 20.51% had PVs. Somatic mutation analysis tumor tissues revealed frequent PTEN PIK3CA ARID1A TP53 Notably, mean age with did not significantly differ from those without, either...
Tissue from 11 cases of cervical cancer positive for human papillomavirus (HPV) type 16 DNA and 69 pelvic lymph nodes the same patients were examined HPV mRNA E6/E7 genes. Five tumors squamous, 3 adeno- adenosquamous carcinoma. From primary extirpated RNA or was subjected to PCR RT-PCR. Three transcription profiles (only E6*I, E6*I E6*II full-length E6-E7 plus both spliced transcripts) found in all DNA-positive tumors. total analyzed 28 mRNA. 7 additional samples. Cytokeratin 19 these nodes,...
Endometrial carcinoma (ECa) is one of the most common neoplasia female genital tract. The phosphatase and tensin (PTEN) homolog frequently mutated tumor suppressor gene in endometrial carcinoma. PTEN encodes a phosphatase, key regulatory enzyme involved signal transduction pathway that regulates cell growth, migration apoptosis. study evaluates an association between morphological appearance hyperplasia ECa, presence variations, protein´s level intracellular localization. A total 67 archived...
Delayed replication of human cytomegalovirus (CMV) was initiated in embryonic fibroblasts using partially ultraviolet light-inactivated virus stock. Cellular [high molecular weight (HMW)] DNA extracted from CMV-infected cell cultures demonstrated a substantial increase transforming activity after introduction into hamster embryo relative to HMW mock-infected cells. The varied between 0.01 and 0.25 foci/micrograms DNA. isolated cells initiation viral synthesis significant decrease the...
Abstract Cowden syndrome (CS) is a rare genetic condition due to the various germline mutations in phosphatase and tensin homologue on chromosome ten ( PTEN ) tumour suppressor gene. As result, CS characterised by an increased risk of developing benign malignant tumours, such as thyroid, breast, endometrial urogenital neoplasms, well gastrointestinal tract tumours. However, neuroendocrine association with not elucidated yet. We present case 46-year-old male patient diagnosed testicular...
Abstract Background Abortion and fetal death are common in fetuses with holoprosencephaly, so genetic examinations often have to be made a post‐mortem setting. The efficiency of the conventional karyotyping using cultured fibroblasts these situations is limited due frequent culture failure. In current study, archived cases where evaluation was requested sufficient frozen material available, were reevaluated quantitative fluorescence polymerase chain reaction (QF‐PCR) technique. Methods...
The authors present a case of 1st trimester miscarriage where an early, complete hydatidiform mole was clinically suspected. Histopathological and immunohistochemical analyses excluded mole, but the histomorphological profile in concordance with partial mole. Genetic analysis based on biparental genome composition, further genetic detected trisomy chromosome 16. Trisomy 16 is frequent cause abortions may lead to highly abnormal placental histomorphology mimicking are crucial for proper...
The aim of the study was genetic characterization a set cases with an unclear morphological profile placental tissue suspected partial hydatidiform mole.This work presents results analysis group 10 patients various clinical manifestations reproductive loss, where mole on basis histopathological examination. composition genome products conception determined by short tandem repeats (STR) genotyping using commercial kit;Devyser Compact v3 (Devyser).Out analyzed cases, five had diandric...
A case of double trisomy 16 and 22 in the second pregnancy loss is presented. DNA analyses (short tandem repeats genotyping) miscarriage specimen was indicated because ultrasound suspicion partial hydatidiform mole. After mole exclusion, further focused on most common aneuploidies causing loss, detected product conception. The couple referred to clinical genetic consultation normal parental karyotypes were proved. For explanatory purposes, archived material from first analyzed chromosome 18...
The authors present a case of partial hydatidiform mole where DNA analysis (STR - short tandem repeat genotyping) showed triandric monogynic tetraploid genome composition with XXXY gonosomal complement. This genetic finding clinicopathologically correlates mole, although it is rare in comparison the typical, diandric triploid moles. definitively confirmed suspected diagnosis mole. To exclude possibility that molar pregnancy represented retained products conception after elective termination,...