Presynaptic inhibition of primary muscle spindle (group Ia) afferent terminals in motor nuclei the spinal cord plays an important role regulating output and is produced by a population GABAergic axon known as P boutons. Despite extensive investigation, cells that mediate this control have not yet been identified. In work, we use immunocytochemistry with confocal microscopy EM to demonstrate boutons can be distinguished from other ventral horn rat mouse their high level glutamic acid...

The long control region (LCR) and the E2 protein of human papillomaviruses (HPV) are most important viral factors regulating transcription oncogenes E6 E7. Sequence variation within these genomic regions may have an impact on oncogenic potential virus. in LCR gene papillomavirus type 16 (HPV-16) isolates originating from cervical cancer patients East Hungary was studied. In 30 samples, sequencing and/or single-strand conformation polymorphism analysis revealed variants belonging to European...

Survivin has recently been identified as a novel member of the inhibitor apoptosis (IAP) gene family. The product this not only suppresses but also controls cell division. is undetectable in most terminally differentiated normal tissues expressed embryonic and fetal organs present malignant tumours. Human papillomaviruses (HPV) are thought to play an important role development cervical cancer. By interfering cycle, viral oncoproteins (E6 E7) can induce immortalization host cell....

Background: Survivin, a novel member of the inhibitor apoptosis family, plays an important role in cell cycle regulation. A common polymorphism at survivin gene promoter (G/C position 31) was shown to be correlated with expression cancer lines. Aim: To investigate whether this could involved development human papillomavirus (HPV)-associated cervical carcinoma. Methods: Survivin detected patients cancer, equivocal cytological atypia and control population using polymerase chain reaction...

Abstract Flow cytometry enables the sequential determination of calcium levels in millions stimulated lymphocytes over a short period time. Current algorithms available are not suitable for statistical analysis this large amount data. The authors aimed to develop robust algorithm that fits function median values measured data and provides an opportunity comparison between different calcium‐flux measurements. alteration signal was monitored CD4+ cells loaded with binding fluorescent dyes...

The human papillomavirus (HPV) life cycle is closely linked to keratinocyte differentiation. Oncogenic HPV infection has been shown hamper the normal differentiation of keratinocytes; however, underlying mechanisms responsible for this phenomenon are yet be clarified. Here, we aimed study effects HPV16 E6 and E7 oncogenes on expression involucrin (IVL), an established marker differentiation, in foreskin (HFK) cells.The HFK cells by serum high calcium significantly increased both mRNA protein...

Abstract Background The Src family tyrosine kinases (SFK) are cellular regulatory proteins that influence cell adhesion, proliferation, invasion and survival during tumor development. Elevated activity of was associated with increased proliferation invasivity in human papillomavirus (HPV)-associated malignancies; therefore, transduced foreskin keratinocytes (HFK) were used to investigate whether SFK activation is a downstream effect papillomaviral oncoproteins. Activation ubiquitously...

Abstract Cervical specimens collected from 163 cytologically healthy women were screened for the presence of human papillomavirus (HPV) DNA and anti‐HPV secretory IgA antibodies. HPV was detected by a general primer mediated polymerase chain reaction (PCR), which amplifies conserved region L1 ORF genital HPVs. The PCR products typed restriction enzyme digestion. A total 35 samples (21.5%) positive (13 6, 6 16, 3 18, 13 untypeable X). positivity significantly higher among under 25 years age...

Abstract Sera of blood donors were investigated by a peptide ELISA and indirect immunofluorescence assay to assess the prevalence HHV‐8 infection in Hungarian population. A 14 amino acid long synthetic oligopeptide from carboxyterminus orf65/small virus capsid antigen was used as ELISA. results confirmed recombinant orf65 Western blot. Antibodies latent nuclear detected assay. Nine 12 sera obtained patients with classical Kaposi sarcoma reactive whereas all positive immunofluorescence. Four...

Abstract About one‐third of human papillomavirus (HPV) types infect the anogenital tract. High‐risk genital HPV (such as 16, 18, 31, 33, and 35) are linked causally to development cervical cancer. The long control region (LCR) genome regulates replication transcription viral genome. In this study, functional significance nucleotide sequence variation within LCR 31 was investigated. amplified by polymerase chain reaction (PCR) from 41 positive samples Hungarian women. A phylogenetic tree...

Prognostic evaluation of HPV-16 genome status the pelvic lymph nodes, integration and p53 codon 72 polymorphism in cervical cancer.Prospective cohort study.Department Gynaecological Oncology, University Debrecen, Hungary.Thirty-nine patients with positive cancer.Primary tumour specimens 39 cancer primary were subjected to multiplex polymerase chain reaction using E1/E2, E7 allele-specific primers. Pelvic nodes same also tested for presence DNA its E1/E2 ORF specific primers,...

ABSTRACT The type specificity of the human papillomavirus (HPV) Hybrid Capture Tube (HCT) test was evaluated by using typing with PCR (MY09-MY11)-restriction fragment length polymorphism (RFLP) and sequencing. All samples HCT positive for only low-risk HPV ( n = 15) or high-risk 102) were confirmed, whereas 9 12 double-positive contained types as determined PCR-RFLP. Several (HPV-53, -58, -62, -66, -CP8304, -MM4) not included in indeed detected, indicating a broader detection range retained...

The role of A/G polymorphism at nucleotide - 1082 in the interleukin-10 (IL-10) promoter was assessed by following disease course 253 patients who had a routine diagnostic Hybrid Capture human papillomavirus (HPV) test because cytologic or colposcopic abnormalities uterine cervix. At baseline, 97 (78%) 125 high-risk HPV-positive and 83 (65%) 128 HPV-negative equivocal atypia classified as P3 Papanicolaou classification, rest mild with results no oncogenic significance. In HPV-infected...

miRNAs and lncRNAs can regulate cellular biological processes both under physiological pathological conditions including tumour initiation progression. Interactions between differentially expressed diverse RNA species, as a part of complex intracellular regulatory network (ceRNA network), may contribute also to the pathogenesis HPV-associated cancer. The purpose this study was investigate global expression changes miRNAs, mRNAs driven by E6 E7 oncoproteins HPV16, construct corresponding...

Abstract Background High-risk human papillomaviruses (HPVs) are responsible for the development of cervical and other anogenital cancers. Intratype sequence variants certain high-risk HPV types (e.g. 16, 18 31) thought to have different oncogenic potential, partly due nucleotide variation in viral long control region (LCR). The LCR has an important role regulation replication transcription. purpose this study was explore 33 intratype Hungary see whether there differences transcriptional...