Mouths offer the perfect environments for microbial cell formation, promoting growth of biofilms, example, on teeth. Dental biofilm exhibits strong resistance to antibiotics and is a cause many dental diseases. Common strategies removal involve addition high concentrations hydrogen peroxide (H2O2), which increases tooth sensitivity, or mechanical procedures. Here, we report different approach based self-propelled micromachines with antibacterial activity degradation biofilm. Such microrobots...

With strong antimicrobial properties, citral has been repeatedly reported to be the dominant component of lemongrass essential oil. Here, we report on a comparison and anticancer activity The oil was prepared by vacuum distillation fresh Cymbopogon leaves, with yield 0.5% (w/w). Citral content measured gas chromatography/high-resolution mass spectrometry (GC-HRMS) determined 63%. Antimicrobial tested broth dilution method, showing against all bacteria fungi. up 100 times more active than...

It is generally recognized that the stability of nanoparticles (NPs) has a great impact on their potential biological applications. Despite this, very few studies have investigated change in toxicity NPs over time but none studied periodic physicochemical changes contributing to it. To address we analyzed effects long-term storage green synthesized silver (AgNPs) directly influences antimicrobial durability. Light-induced slow synthesis AgNPs was carried out using Saraca asoca aqueous leaf...

Silybin is considered to be the main biologically active component of silymarin. Its oxidized derivative 2,3-dehydrosilybin typically occurs in silymarin small, but non-negligible amounts (up 3%). Here, we investigated detail complex biological activities silybin and optical isomers. Antioxidant pure stereomers A B 2,3-dehydrosilybin, as well their racemic mixtures, were by using oxygen radical absorption capacity (ORAC) cellular antioxidant activity (CAA) assay. All substances efficiently...

The inhibition and eradication of oral biofilms is increasingly focused on the use plant extracts as mouthwashes toothpastes adjuvants. Here, we report chemical composition antibiofilm activity 15 methanolic Iris species against both mono-(Pseudomonas aeruginosa, Staphylococcus aureus) multi-species (Streptococcus gordonii, Veillonella parvula, Fusobacterium nucleatum subsp. nucleatum, Actinomyces naeslundii). phytochemical profiles pallida s.l., versicolor L., lactea Pall., carthaliniae...

Abstract Herbal-based dietary supplements have become increasingly popular. The extract from milk thistle ( Silybum marianum) , is often used for the treatment of liver diseases. However, serious concerns exist regarding efficacy, composition, as well safety these over-the-counter preparations. Therefore, aim present study was to investigate composition chemical and biological 26 thistle-based purchased both U.S. Czech markets between 2016 2017. focused on a determination active ingredients,...

Mycotoxins found in randomly selected commercial milk thistle dietary supplement were evaluated for their toxicity silico and vitro. Using methods, the basic physicochemical, pharmacological, toxicological properties of mycotoxins predicted using ACD/Percepta. The vitro cytotoxicity individual was determined mouse macrophage (RAW 264.7), human hepatoblastoma (HepG2), embryonic kidney (HEK 293T) cells. In addition, we studied bioavailability potential silibinin utilizing an transwell system...

Although some metallic nanoparticles (NPs) are commonly used in the food processing plants as nanomaterials for packaging, or coatings on handling equipment, little is known about antimicrobial properties of palladium (PdNPs) and platinum (PtNPs) their potential use industry. In this study, common food-borne pathogens Salmonella enterica Infantis, Escherichia coli, Listeria monocytogenes Staphylococcus aureus were tested. Both NPs reduced viable cells with log10 CFU reduction 0.3–2.4 0.8–2.0...

Salvia officinalis L., Sambucus nigra Matricaria chamomilla Agrimonia eupatoria Fragaria vesca L. and Malva sylvestris are plants that have a long tradition in European folk medicine. To this day, they part of medicinal teas or creams help with the healing skin wounds treatment respiratory intestinal infections. However, so far these not been investigated more deeply than their direct antibacterial effect. Our research is focused on adjuvants inhibit mechanism antibiotic resistance modulate...

Numerous in vitro assays are used to characterize the antioxidant properties of natural-based matrices. However, many them generate contradictory and non-compliant results. In our study, we focused on characterization traditionally biochemical (2,2′-azino-bis-(3-ethylbenzothiazoline-6 sulfonic acid) (ABTS), Oxygen Radical Absorption Capacity (ORAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH)) cellular (CAA) tests a broad set milk thistle dietary supplements containing silymarin. addition 26...

Although stinging nettle (Urtica dioica) has been shown to reduce HM (heavy metal) content in soil, its wider phytoremediation potential neglected. Urtica dioica was cultivated soils contaminated with HMs or polychlorinated biphenyls (PCBs). After four months, up 33% of the less chlorinated and 8% (Zn, Pb, Cd) had removed. Bacteria were isolated from plant tissue, endophytic bacteria Bacillus shackletonii Streptomyces badius have most significant effect. These demonstrated not only benefits...

The aim of this work is to increase the efficiency biodegradation polychlorinated biphenyls (PCBs) by introduction bacterial genes into plant genome. For purpose, we selected bphC gene encoding 2,3-dihydroxybiphenyl-1,2-dioxygenase from Pseudomonas testosteroni B-356 be cloned tobacco plants. dihydroxybiphenyldioxygenase enzyme third in biphenyl degradation pathway, and its unique function cleavage biphenyl. Three different constructs were designed prepared E. coli: being fused with...

Silychristin A is the second most abundant compound of silymarin. Silymarin complex was previously described as an antioxidant with multidrug resistance modulation activity. Here, results a classical biochemical assay (ORAC) were compared cellular evaluating capacity pure silychristin and its derivatives (anhydrosilychristin, isosilychristin 2,3-dehydrosilychristin A). All tested compounds acted antioxidants within cells, but 2,3-dehydro- anhydro almost twice potent other compounds. Similar...

Antibiotic resistance is currently a serious health problem. Since the discovery of new antibiotics no longer seems to be sufficient tool in fight against multidrug-resistant infections, adjuvant (combination) therapy gaining importance as well reducing bacterial virulence. Silymarin complex flavonoids and flavonolignans known for its broad spectrum biological activities, including ability modulate drug cancer. This work aimed test eleven, optically pure silymarin their reverse multidrug...

Cannabidiol (CBD) is the non-psychoactive component of plant Cannabis sativa (L.) that has great anti-inflammatory benefits and wound healing effects. However, its high lipophilicity, chemical instability, extensive metabolism impair bioavailability clinical use. Here, we report on preparation a human cornea substitute in vitro validate this for evaluation drug penetration. CBD nanoemulsion was developed evaluated stability biological activity. The physicochemical properties were maintained...

The stem juices from Musa × paradisiaca L. banana plants cultivated in their original natural habitat Vietnam and those a greenhouse the Czech Republic were investigated for presence of phytochemicals with antidiabetic potency. Respective bioactivities these also determined. Sample screening using ultrahigh performance liquid chromatography coupled tandem high-resolution mass spectrometry (UHPL-HRMS/MS) method showed some differences pattern bioactive compounds, both terms number...

As a herb used in traditional medicine, Agrimonia eupatoria L. contains number of secondary metabolites with beneficial properties. However, various factors such as species, ontogenesis, part plant and its processing can influence the overall bioactivity. Here, we assess effect variability on phytochemical composition bioactive potential agrimony. A complex set agrimony samples was extracted, analyzed by UHPLC-HRMS/MS screened for selected compounds (n=195). The detected profiles, especially...

Arrayan and peumo fruits are commonly used in the traditional medicine of Chile. In this study, concentration extracts halving bacterial viability biofilms formation disruption drug-sensitive drug-resistant strains Staphylococcus aureus Pseudomonas aeruginosa was determined. The chemical composition analyzed by high-resolution liquid chromatography coupled with mass spectrometry (U-HPLC/MS). arrayan extract (Inhibitory IC50 0.35 ± 0.01 mg/mL) more effective than (IC50 0.53 0.02 inhibition S....