A5055528010- Wnt/β-catenin signaling in development and cancer
- Genetics and Neurodevelopmental Disorders
- Genetic and Kidney Cyst Diseases
- Melanoma and MAPK Pathways
- Neurogenesis and neuroplasticity mechanisms
- Cell Adhesion Molecules Research
- Synthesis and biological activity
- Retinoids in leukemia and cellular processes
- MicroRNA in disease regulation
- Cerebrospinal fluid and hydrocephalus
- Nuclear Receptors and Signaling
- Synthesis of Organic Compounds
- Nutrition, Genetics, and Disease
- RNA Research and Splicing
- Lattice Boltzmann Simulation Studies
- Child and Adolescent Health
- RNA modifications and cancer
- GDF15 and Related Biomarkers
- Protein Hydrolysis and Bioactive Peptides
- Protein Degradation and Inhibitors
- Congenital heart defects research
- Peptidase Inhibition and Analysis
- Neuroscience and Neuropharmacology Research
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
Masaryk University
2015-2022
Karolinska Institutet
2016
Flow cytometry is a powerful method, which widely used for high‐throughput quantitative and qualitative analysis of cells. However, its straightforward applicability extracellular vesicles (EVs) mainly exosomes hampered by several challenges, reflecting mostly the small size these (exosomes: ~80–200 nm, microvesicles: ~200–1,000 nm), their polydispersity, low refractive index. The current best most protocol beads‐free flow uses ultracentrifugation (UC) coupled with floatation in sucrose...
Abstract WNTs are lipid-modified proteins that control multiple functions in development and disease via short- long-range signaling. However, it is unclear how these hydrophobic molecules spread over long distances the mammalian brain. Here we show WNT5A produced by choroid plexus (ChP) of developing hindbrain, but not telencephalon, both mouse human. Since ChP produces secretes cerebrospinal fluid (CSF), examine presence CSF find associated with lipoprotein particles rather than exosomes....
RNF43 is an E3 ubiquitin ligase and known negative regulator of WNT/β-catenin signaling. We demonstrate that also a noncanonical WNT5A-induced signaling in human cells. Analysis the interactome using BioID immunoprecipitation showed can interact with core receptor complex components dedicated to Wnt pathway such as ROR1, ROR2, VANGL1, VANGL2. triggers VANGL2 ubiquitination proteasomal degradation clathrin-dependent internalization ROR1 inhibits ROR2 activation. These activities are...
ABSTRACT The choroid plexus (ChP) produces cerebrospinal fluid and forms an essential brain barrier. ChP tissues form in each ventricle, one adopting a distinct shape, but remarkably little is known about the mechanisms underlying development. Here, we show that epithelial WNT5A crucial for determining fourth ventricle (4V) morphogenesis size mouse. Systemic Wnt5a knockout, or forced overexpression beginning at embryonic day 10.5, profoundly reduced However, expression was enriched...
ABSTRACT The choroid plexus (ChP) produces cerebrospinal fluid and forms a critical barrier between the brain circulation. While ChP in each ventricle, it adopts different shape one remarkably little is known about mechanisms underlying its development. Here, we show that epithelial WNT5A for determining fourth ventricle (4V) morphogenesis size. Systemic Wnt5a knockout, or forced overexpression beginning at E10.5, profoundly reduced size development of all ventricles. However, conditional...
Abstract RNF43 is a E3 ubiquitin ligase and known negative regulator of WNT/β-catenin signaling. We demonstrate that also noncanonical WNT5A-induced signaling in human cells. Analysis the interactome using BioID immunoprecipitation showed can interact with core receptor complex components dedicated to Wnt pathway such as ROR1, ROR2, VANGL1 VANGL2. triggers VANGL2 ubiquitination proteasomal degradation clathrin-dependent internalization ROR1 receptor. This activity physiologically relevant...