A5066366245- Testicular diseases and treatments
- Ovarian cancer diagnosis and treatment
- Histone Deacetylase Inhibitors Research
- Sarcoma Diagnosis and Treatment
- Cancer Cells and Metastasis
- PARP inhibition in cancer therapy
- Cancer Mechanisms and Therapy
- Colorectal Cancer Treatments and Studies
- Protein Degradation and Inhibitors
- Cancer, Hypoxia, and Metabolism
- Cancer Genomics and Diagnostics
- Urologic and reproductive health conditions
- Prostate Cancer Treatment and Research
- Neuroblastoma Research and Treatments
- Multiple Myeloma Research and Treatments
- Epigenetics and DNA Methylation
- Glutathione Transferases and Polymorphisms
- Chemotherapy-induced organ toxicity mitigation
- Genetic factors in colorectal cancer
- Cancer, Stress, Anesthesia, and Immune Response
- Medicinal Plant Research
- Bioactive Compounds and Antitumor Agents
- Beetle Biology and Toxicology Studies
- Agricultural and Biological Research
- Sesame and Sesamin Research
Biomedical Research Center of the Slovak Academy of Sciences
2018-2025
Cancer Research Institute of the Slovak Academy of Sciences
2018-2025
Comenius University Bratislava
2020-2022
Slovak Academy of Sciences
2018-2021
Efficiency of colorectal carcinoma treatment by chemotherapy is diminished as the resistance develops over time in patients. The same holds true for 5-fluorouracil, drug used first line carcinoma.Chemoresistant derivative HT-29 cells was prepared long-term culturing increasing concentration 5-fluorouracil. Cells were characterized viability assays, flow cytometry, gene expression arrays and kinetic imaging. Immunomagnetic separation isolation subpopulations positive cancer stem cells-related...
Cisplatin resistance in testicular germ cell tumors (TGCTs) is a clinical challenge. We investigated the underlying mechanisms associated with cancer stem (CSC) markers and modalities circumventing chemoresistance. Chemoresistant models (designated as CisR) of human embryonal carcinoma lines NTERA-2 NCCIT were derived characterized using flow cytometry, gene expression, functional protein arrays. Tumorigenicity was determined on immunodeficient mouse model. Disulfiram used to examine...
Cisplatin is the main systemic treatment modality for male type II germ cell tumors (GCTs). Although generally very effective, 5%-10% of patients suffer from cisplatin-resistant disease. Identification driving mechanisms resistance will enable improved risk stratification and development alternative treatments.
Patients with treatment-refractory/relapsing germ cell tumors (GCTs) have a dismal prognosis due to lack of any effective therapy. Moreover, the efficacy newly approved targeted therapies remains unexplored for cisplatin-resistant GCTs. Previously, it was demonstrated that folate receptor α (FRα) is overexpressed in many tumor types and efficiently by antibody-drug conjugate (ADC) mirvetuximab soravtansine (MIRV) cancers. We hypothesized FRα represents an attractive target treating...
Cisplatin resistance of ovarian yolk sac tumors (oYST) is a clinical challenge due to dismal patient prognosis, even though the disease extremely rare. We investigated potential association between cisplatin and cancer stem cell (CSC) markers in chemoresistant oYST cells targeting strategies overcome oYST.Chemoresistant were derived from chemosensitive human by cultivation vitro. Derivative characterized chemoresistance, functional assays, flow cytometry, gene expression protein arrays...
The majority of patients with testicular germ cell tumors (GCTs) can be cured cisplatin-based chemotherapy. However, for a subset present cisplatin-refractory disease, which confers poor prognosis, the treatment options are limited. Novel therapies therefore urgently needed to improve outcomes in this challenging patient population. It has previously been shown that Wnt/β-catenin signaling is active GCTs suggesting its inhibitors LGK974 and PRI-724 may show promise management GCTs. We herein...
Germ cell tumors (GCTs) usually represent efficiently curable neoplasms due to their chemosensitivity platinum-based therapeutic regimen. However, some patients develop resistance and succumb disease. Novel approaches are therefore needed for these patients. It has previously been demonstrated that poly (ADP-ribose) polymerase (PARP) expression is upregulated in GCTs compared with normal testis tissue. Therefore, PARP was analyzed GCT lines xenografts it examined whether its inhibition by...
Apoptosis is a strictly regulated process essential for preservation of tissue homeostasis. This study aimed to evaluate expression apoptosis inducing factor (AIF) in testicular germ cell tumors (GCTs) and correlate patterns with clinicopathological variables. Formalin-fixed paraffin-embedded specimens non-neoplastic GCTs obtained from 216 patients were included the study. AIF was detected by immunohistochemistry, scored multiplicative quickscore method (QS). Normal exhibits higher...
Introduction Majority of testicular germ cell tumours (TGCTs) respond well to cisplatin (CDDP)-based chemotherapy. However, patients with refractory disease have limited treatment modalities associated poor prognosis. CDDP resistance is a complex phenomenon, and there need for overcome the chemoresistance in TGCTs. Material methods CDDP-resistant variants human TGCTs lines JEG-3, NTERA-2, NOY-1 were derived by cultivation gradually increasing drug concentrations 6 months. Chemoresistance...
e17013 Background: Multiple relapsed/refractory GCTs patients have extremely poor prognosis, therefore, new treatment strategies are warranted. Previously, we showed, that cisplatin resistant testicular overexpress aldehyde-dehydrogenase (ALDH) isoforms and inhibition of ALDH activity by disulfiram is associated with reconstitution sensitivity in vitro as well animal model. This study aimed to determine the efficacy toxicity inhibitor combination multiple germ cell cancer. Methods: Twelve...