A5075024030- Carcinogens and Genotoxicity Assessment
- DNA Repair Mechanisms
- Effects of Radiation Exposure
- Sperm and Testicular Function
- Effects and risks of endocrine disrupting chemicals
- Epigenetics and DNA Methylation
- Genetically Modified Organisms Research
- Heavy Metal Exposure and Toxicity
- Potato Plant Research
- CRISPR and Genetic Engineering
- Birth, Development, and Health
- Mitochondrial Function and Pathology
- Osteoarthritis Treatment and Mechanisms
- Renal and related cancers
- Radiation Therapy and Dosimetry
- Radioactive contamination and transfer
- Reproductive Biology and Fertility
- MicroRNA in disease regulation
- Drug Transport and Resistance Mechanisms
- Air Quality and Health Impacts
- Mesenchymal stem cell research
- bioluminescence and chemiluminescence research
- Attention Deficit Hyperactivity Disorder
- Circular RNAs in diseases
- Nanoparticles: synthesis and applications
Utah State University
2006-2025
Universidad de Navarra
2025
Norwegian Institute of Public Health
2011-2023
Norwegian University of Life Sciences
2014-2023
University of Oslo
2023
The Research Council of Norway
2023
Litron Laboratories (United States)
2014
Nordic Bioscience (Denmark)
2006
Recently, we showed that silver nanoparticles (AgNPs) caused apoptosis, necrosis and DNA strand breaks in different cell models vitro. These findings warranted analyses of their relevance vivo. We investigated the genotoxic potential gene expression profiles particles nano- (Ag20, 20 nm) submicron- (Ag200, 200 size titanium dioxide (TiO2-NPs, 21 selected tissues from exposed male mice including gonades. A single dose 5 mg/kg bw was administered intravenously to derived C57BL6 (WT)...
Paternal exposure to high levels of radioactivity causes heritable germline minisatellite mutations. However, the effect more general paternal exposures, such as cigarette smoking, on mutations remains unexplored. We analyzed two most commonly used loci (CEB1 and B6.7) identify in blood samples complete mother-father-child triads from Norwegian Mother Child Cohort Study (MoBa). The presence was subsequently related lifestyle factors, including smoking before partner became pregnant....
The quality of germ cell DNA is critical for the fate offspring, yet there limited knowledge repair capabilities such cells. One main pathways base excision (BER) which initiated by glycosylases that excise damaged bases, followed incision generated abasic (AP) sites. We have studied human and rat methylpurine-DNA glycosylase (MPG), uracil-DNA (UNG), major AP endonuclease (HAP1/APEX) in male Enzymatic activities western analyses indicate these enzymes are present cells amounts at least as...
Abstract Background Testicular germ cell tumors (TGCTs) respond well to cisplatin-based chemotherapy and show a low incidence of acquired resistance compared most somatic tumors. The reasons for these specific characteristics are not known in detail but seem be multifactorial. We have studied gene expression profiles testicular colon cancer derived lines treated with cisplatin. main goal this study was identify novel their functional categories the biochemical pathways that associated TGCT...
Base excision repair (BER) is a major pathway for removal of DNA base lesions and maintenance genomic stability, which essential in cancer prevention. glycosylases recognize remove specific the first step BER. The existence number these enzymes with overlapping substrate specificities has been thought to be reason why single knock-out models individual are not prone. In this work we have characterized NEIL1 NEIL2 (Neil1 -/- /Neil2 -/-) double NEIL1, NEIL3 /Neil3 triple mouse models....
Abstract Even today, 70 years after Hiroshima and accidents like in Chernobyl Fukushima, we still have limited knowledge about the health effects of low dose rate (LDR) radiation. Despite their human relevance occupational accidental exposure, only few animal studies on genotoxic chronic LDR radiation been performed. Selenium (Se) is involved oxidative stress defence, protecting DNA other biomolecules from reactive oxygen species (ROS). It hypothesised that Se deficiency, as it occurs...
Oxidative damage in testicular DNA is associated with poor semen quality, reduced fertility and increased risk of stillbirths birth defects. These lesions are predominantly removed by base excision repair. Cellular extracts from human rat cells three enriched populations male germ (primary spermatocytes, round spermatids elongating/elongated spermatids) all showed proficient excision/incision 5‐hydroxycytosine, thymine glycol 2,6‐diamino‐4‐hydroxy‐5‐formamidopyrimidine. containing...
Octyl methoxycinnamate (OMC) is one of the most widely used sunscreen ingredients. To analyze biological effects OMC, an in vitro approach was implying ultraviolet (UV) exposure two human cell lines, a primary skin fibroblast (GM00498) and breast cancer (MCF-7) lines. End points include viability assessment, assay cyclobutane pyrimidine dimers (CPDs) oxidated DNA lesions using alkaline elution lesion-specific enzymes, gene expression analysis panel 17 damage-responsive genes. We observed...
Background Spermatozoal DNA damage is associated with poor sperm quality, disturbed embryonic development and early loss, some genetic diseases originate from paternal de novo mutations. We previously reported repair of bulky DNA-lesions in rodent testicular cells. Methodology/Principal Findings studied the fate lesions male germ line. B[a]PDE-N2-dG adducts were determined by liquid chromatography-tandem mass spectrometry, mutations measured cII-transgene, Big Blue®mice exposed to...
Abstract Mechanistic toxicology is gaining weight for human health risk assessment. Different mechanistic assays are available, such as the comet assay, which detects DNA damage at level of individual cells. However, conventional alkaline version only strand breaks and alkali-labile sites. We have validated two modifications in vitro assay to generate information: (1) use DNA-repair enzymes (i.e., formamidopyrimidine glycosylase, endonuclease III, 8-oxoguanine glycosylase I alkyladenine...
The health of the offspring depends on genetic constitution parental germ cells. paternal genome appears to be important; e.g., de novo mutations in some genes seem arise mostly from father, whereas epigenetic modifications DNA and histones are frequent gonads. Environmental contaminants which may affect integrity cells comprise polycyclic aromatic hydrocarbon, benzo[a]pyrene (B[a]P). B[a]P has received much attention due its ubiquitous distribution, carcinogenic mutagenic potential, also...
The alkaline comet assay, in vivo and vitro, is currently used several areas of research regulatory genotoxicity testing. Several efforts have been made order to decrease the inter-experimental inter-laboratory variability increase reliability assay. In this regard, lysis conditions are considered as one critical variables need be further studied. Here, we tested different times (from no 1 week) two solutions human lymphoblast (TK6) cells unexposed or exposed X-rays. Similar % tail DNA...
Pregnant women and their fetuses are exposed to multiple toxic metals that together with variations in essential element levels may alter epigenetic regulation, such as DNA methylation. The aim of the study was investigate associations between gestational elements mixtures thereof, global methylation pregnant newborn children. Using 631 mother-child pairs from a prospective birth cohort (The Norwegian Mother, Father Child Cohort Study), we measured maternal blood concentration (gestation...