A5021264532- Carcinogens and Genotoxicity Assessment
- DNA Repair Mechanisms
- Effects and risks of endocrine disrupting chemicals
- Toxic Organic Pollutants Impact
- Pesticide Exposure and Toxicity
- Computational Drug Discovery Methods
- Nutritional Studies and Diet
- Dye analysis and toxicity
- Genetically Modified Organisms Research
- Heavy Metal Exposure and Toxicity
- Diet and metabolism studies
- Animal testing and alternatives
- PARP inhibition in cancer therapy
- Free Radicals and Antioxidants
- Gut microbiota and health
- Mycotoxins in Agriculture and Food
- Celiac Disease Research and Management
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- Chromium effects and bioremediation
- Pharmacogenetics and Drug Metabolism
- Meat and Animal Product Quality
- Chemical Safety and Risk Management
- Plant Genetic and Mutation Studies
- Insect Resistance and Genetics
Toxalim Research Centre in Food Toxicology
2015-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2010-2025
Université Toulouse III - Paul Sabatier
2008-2024
École Nationale Vétérinaire de Toulouse
2010-2024
Université de Toulouse
2012-2024
Institut National de la Recherche Agronomique
2012-2015
Institut National Polytechnique de Toulouse
2013-2015
Institut National de la Recherche Agronomique du Niger
2014
Inserm
2008
Centre National de la Recherche Scientifique
2000-2007
The efficient repair of DNA double-strand breaks (DSBs) is critical for the maintenance genomic integrity. In mammalian cells, nonhomologous end-joining process that represents predominant pathway relies on DNA-dependent protein kinase (DNA-PK) and XRCC4-DNA ligase IV complex. Nonetheless, several in vitro vivo results indicate cells use more than a single mechanism. While searching DNA-PK-independent activity, we found pretreatment DNA-PK-proficient -deficient rodent with an inhibitor...
Epidemiology shows that red and processed meat intake is associated with an increased risk of colorectal cancer. Heme iron, heterocyclic amines, endogenous N-nitroso compounds (NOC) are proposed to explain this effect, but their relative contribution unknown. Our study aimed at determining, nutritional doses, which the main factor involved proposing a mechanism cancer promotion by meat. The part heme iron (1% in diet), amines (PhIP + MeIQx, 50 25 μg/kg NOC (induced NaNO₂+ NaNO₂; 0.17 0.23...
The capability of the flame retardants tetrabromobisphenol A (TBBPA) and tetrachlorobisphenol (TCBPA) to activate peroxysome proliferator–activated receptors (PPARs) α, β, γ estrogen (ERs) α β has been recently investigated, but activity their biotransformation products lower molecular weight analogues formed in environment remains unexplored. aim this study was investigate relationship between degree halogenation BPA affinity towards human PPARγ ERs characterize active metabolites major...
In Escherichia coli, the biosynthetic pathways of several small iron-scavenging molecules known as siderophores (enterobactin, salmochelins and yersiniabactin) a genotoxin (colibactin) are to require 4′-phosphopantetheinyl transferase (PPTase). Only two PPTases have been clearly identified: EntD ClbA. The gene coding for is part core genome E. whereas ClbA encoded on pks pathogenicity island which codes colibactin. Interestingly, physically associated with high (HPI) in subset highly...
Abstract CD8 T cells are emerging as important players in multiple sclerosis (MS) pathogenesis, although their direct contribution to tissue damage is still debated. To assess whether autoreactive can contribute the pronounced loss of oligodendrocytes observed MS plaques, we generated mice which model Ag influenza hemagglutinin selectively expressed oligodendrocytes. Transfer preactivated hemagglutinin-specific led inflammatory lesions optic nerve, spinal cord, and brain. These lesions,...
In vitro genotoxicity tests used in regulatory toxicology studies are sensitive, but the occurrence of irrelevant positive results is high compared with carcinogenicity rodents. Current also often limited by relatively low throughput. The aim this study was to validate an genotoxic assay a 96‐well plate format that allows simultaneous examination cytotoxicity and genotoxicity. test based on quantification phosphorylation histone H2AX (γH2AX), which reflects global insult, using In‐Cell...
Consumers may be simultaneously exposed to several pesticide residues in their diet. A previous study identified the seven most common mixtures which French population was through food consumption 2006. The aim of this investigate if are potentially cytotoxic and genotoxic so, whether compounds a same mixture have combined effect. cytotoxicity genotoxicity were investigated with new assay (γ-H2AX) using four human cell lines (ACHN, SH-SY5Y, LS-174T, HepG2). Mixtures tested at equimolar...
Pyrrolizidine alkaloids (PAs) are secondary metabolites from plants that have been found in substantial amounts herbal supplements, infusions and teas. Several PAs cause cancer animal bioassays, mediated via a genotoxic mode of action, but for the majority PAs, carcinogenicity data lacking. It is assumed risk assessment all same potency as riddelliine, which considered to be one most potent carcinogenic rats. This may overestimate risks, since many expected lower potencies. In this study we...
Abstract The Genetic Toxicology Technical Committee (GTTC) of the Health and Environmental Sciences Institute (HESI) is developing adverse outcome pathways (AOPs) that describe modes action leading to potentially heritable genomic damage. goal was enhance use mechanistic information in genotoxicity assessment by building empirical support for relationships between relevant molecular initiating events (MIEs) regulatory endpoints genetic toxicology. Herein, we present an AOP network links...
We have investigated the effect of single amino acid substitutions conserved arginines on catalytic activities human Ogg1 protein (α-hOgg1-Ser326) (wild-type α-hOgg1). Mutant forms hOgg1 with mutations Arg46→Gln (α-hOgg1-Gln46) and Arg154→His (α-hOgg1-His154) previously been identified in tumors. The mutant proteins α-hOgg1-Gln46 α-hOgg1-His154 were expressed Escherichia coli purified to homogeneity. substrate specificities these wild-type α-hOgg1 using γ-irradiated DNA technique gas...
Heavy metals, such as arsenic (As), antimony (Sb), barium (Ba), cadmium (Cd), cobalt (Co), germanium (Ge), lead (Pb), nickel (Ni), tellurium (Te), and vanadium (V) are widely distributed in the environment food chain. Human exposure to heavy metals through water has been reported by different international agencies. Although some of these essential elements for human growth development, they may also be toxic at low concentrations due indirect mechanisms. In this study, genotoxic cytotoxic...
Humans are involuntarily exposed to hundreds of chemicals that either contaminate our environment and food or added intentionally daily products. These complex mixtures may pose a risk human health. One the goals European Union’s Green Deal zero-pollution ambition for toxic-free is tackle existent gaps in chemical mixture assessment by providing scientific grounds support implementation adequate regulatory measures within EU. We suggest dealing with this challenge by: (1) characterising...