A5075813736- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- Effects and risks of endocrine disrupting chemicals
- Adipose Tissue and Metabolism
- Advanced Chemical Sensor Technologies
- Gut microbiota and health
- Liver Disease Diagnosis and Treatment
- Carcinogens and Genotoxicity Assessment
- Analytical Chemistry and Chromatography
- Mycotoxins in Agriculture and Food
- Toxic Organic Pollutants Impact
- Pesticide Exposure and Toxicity
- Meat and Animal Product Quality
- Molecular spectroscopy and chirality
- Spectroscopy and Chemometric Analyses
- Infant Nutrition and Health
- Bioinformatics and Genomic Networks
- Birth, Development, and Health
- Salivary Gland Disorders and Functions
- Traditional Chinese Medicine Studies
- Nutritional Studies and Diet
- Genetic and phenotypic traits in livestock
- Advanced Proteomics Techniques and Applications
- Liquid Crystal Research Advancements
- Fatty Acid Research and Health
Toxalim Research Centre in Food Toxicology
2016-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2012-2025
Université Toulouse III - Paul Sabatier
2016-2025
École Nationale Vétérinaire de Toulouse
2016-2025
MetaboHUB
2018-2025
Université de Toulouse
2014-2024
Centre National de la Recherche Scientifique
1997-2022
Laboratoire de Recherche en Sciences Végétales
2022
Institut National Polytechnique de Toulouse
2021
Biologie du Fruit et Pathologie
2021
Bisphenol A (BPA) is a well-known endocrine disruptor used to manufacture polycarbonate plastics and epoxy resins. Exposure of pregnant rodents low doses BPA results in pleiotropic effects their offspring.We metabolomics--a method for determining metabolic changes response nutritional, pharmacological, or toxic stimuli--to examine shifts induced vivo by perinatal exposure CD-1 mice.Male offspring born mice that were exposed vehicle 0.025, 0.25, 25 µg BPA/kg body weight/day, from gestation...
Background: Epidemiological evidence suggests a link between pesticide exposure and the development of metabolic diseases. However, most experimental studies have evaluated effects pesticides using individual molecules, often at nonrelevant doses or in combination with other risk factors such as high-fat diets. Objectives: We aimed to evaluate, mice, consequences chronic dietary mixture nontoxic doses, relevant consumers' assessment. Methods: A six commonly used France, i.e., boscalid,...
The metabo-ring initiative brought together five nuclear magnetic resonance instruments (NMR) and 11 different mass spectrometers with the objective of assessing reliability untargeted metabolomics approaches in obtaining comparable profiles. This was estimated by measuring proportion common spectral information extracted from LCMS NMR platforms. Biological samples obtained 2 conditions were analysed partners using their own in-house protocols. Test #1 examined urine adult volunteers either...
Abstract The liver is a key organ of metabolic homeostasis with functions that oscillate in response to food intake. Although and gut microbiome crosstalk has been reported, microbiome-mediated effects on peripheral circadian clocks their output genes are less well known. Here, we report germ-free (GF) mice display altered daily oscillation clock gene expression concomitant change the regulators. Mice exposed microbes typically exhibit characterized activities nuclear receptors, some which...
Peroxisome proliferator activated receptor α (PPARα) acts as a fatty acid sensor to orchestrate the transcription of genes coding for rate-limiting enzymes required lipid oxidation in hepatocytes. Mice only lacking Pparα hepatocytes spontaneously develop steatosis without obesity aging. Steatosis can into non alcoholic steatohepatitis (NASH), which may progress irreversible damage, such fibrosis and hepatocarcinoma. While NASH appears major public health concern worldwide, it remains an...
In suckling mammals, the onset of solid food ingestion is coincident with maturation gut barrier. This ontogenic process driven by colonization intestine microbiota. However, mechanisms underlying microbial regulation intestinal development in early life are not fully understood. Here, we studied co-maturation microbiota (composition and metabolic activity) barrier at suckling-to-weaning transition using a combination experiments vivo (suckling rabbit model), ex (Ussing chambers) vitro...
Infections by multidrug-resistant Enterobacteriaceae (MRE) are life-threatening to patients. The intestinal microbiome protects against MRE colonization, but antibiotics cause collateral damage commensals and open the way colonization subsequent infection. Despite significance of this problem, specific mechanisms that restrict remain largely unknown. Here, performing a multi-omic prospective study hospitalized patients combined with mice experiments, we find Lactobacillus is key, though not...
Inhalation of Aspergillus fumigatus conidia can cause severe aspergillosis in immunosuppressed people. A. produces a large number secondary metabolites, some which are airborne by and whose toxicity to the respiratory tract has not been investigated. We found that spores contain five main compounds, tryptoquivaline F, fumiquinazoline C, questin, monomethylsulochrin trypacidin. Fractionation culture extracts using RP-HPLC LC-MS showed samples containing trypacidin were toxic human A549 lung...
Abstract Motivation In metabolomics, the detection of new biomarkers from Nuclear Magnetic Resonance (NMR) spectra is a promising approach. However, this analysis remains difficult due to lack whole workflow that handles pre-processing, automatic identification and quantification metabolites statistical analyses, in reproducible way. Results We present ASICS, an R package contains complete analyse NMR experiments. It approach identify quantify complex mixture spectrum uses results untargeted...
The World Health Organization classified processed and red meat consumption as "carcinogenic" "probably carcinogenic", respectively, to humans. Haem iron from plays a role in the promotion of colorectal cancer rodent models, association with enhanced luminal lipoperoxidation subsequent formation aldehydes. Here, we investigated short-term effects this haem-induced on mucosal gut homeostasis including microbiome F344 male rats fed haem-enriched diet (1.5 μmol/g) 14–21 days. Changes...
Background The use of pesticides and the related environmental contaminations can lead to human exposure various molecules. In early-life, such exposures could be responsible for adverse developmental effects. However, health risks associated with complex mixtures are currently under-explored. Objective This project aims at answering following questions: What is influence multiple on metabolome? mechanistic pathways involved in metabolic changes observed? Methods Based PELAGIE cohort...
The use of pesticides exposes humans to numerous harmful molecules. Exposure in early-life may be responsible for adverse effects later life. This study aimed assess the metabolic modifications induced pregnant rats and their offspring by a pesticide mixture representative human exposure. Ten were exposed eight pesticides: acetochlor (246 μg/kg bw/d) + bromoxynil (12 carbofuran (22.5 chlormequat (35 ethephon fenpropimorph (15.5 glyphosate imidacloprid (12.5 representing main environmental...
The gut microbiota plays a key role in intestinal development at the suckling-to-weaning transition. objective of this study was to analyze production metabolites by suckling and weaned piglets. We studied piglets raised two separate maternity farms postnatal day 21 same farm. fecal metabolome (1H nuclear magnetic resonance) composition (16S rRNA gene amplicon sequencing) its predicted functions (PICRUSt2) were analyzed during period (postnatal 13) 2 days after weaning 23). relative...
Alzheimer's disease is strongly linked to metabolic abnormalities. We aimed distinguish amyloid-positive people who progressed cognitive decline from those remained cognitively intact. performed untargeted metabolomics of blood samples individuals, before any sign decline, individuals A plasma-derived metabolite signature was developed Supercritical Fluid chromatography coupled with high-resolution mass spectrometry (SFC-HRMS) and nuclear magnetic resonance (NMR) metabolomics. The 2 data...
Abstract Various spectrometric methods can be used to conduct metabolomics studies. Nuclear magnetic resonance (NMR) or mass spectrometry (MS) coupled with separation methods, such as liquid gas chromatography (LC and GC, respectively), are the most commonly techniques. Once raw data have been obtained, real challenge lies in bioinformatics required conduct: (i) processing (including preprocessing, normalization, quality control); (ii) statistical analysis for comparative studies (such...
Capturing produced, consumed, or exchanged metabolites (metabolomics) and the result of gene expression (transcriptomics) require extraction RNA. Multi-omics approaches and, notably, combination metabolomics transcriptomic analyses are required for understanding functional changes adaptation microorganisms to different physico-chemical environmental conditions. A protocol was developed extract total RNA from less than 6 mg a kind phototrophic biofilm: oxygenic photogranules. These granules...
Oxygenic photogranules mainly composed of cyanobacteria and heterotrophic bacteria, have gained attention for their ability to treat wastewater (removal C, N, P) without external aeration. Currently, the metabolic dynamics varying nutrient light conditions in treatment systems remains poorly studied. However, understanding how quickly photogranule metabolism changes, whether this change is temporary or permanent important optimal use photogranules. Here, an NMR-based metabolomics approach...