A5023452339- Nutritional Studies and Diet
- DNA Repair Mechanisms
- Cancer Research and Treatments
- Bacterial Genetics and Biotechnology
- Immune Response and Inflammation
- Salmonella and Campylobacter epidemiology
- Genomics, phytochemicals, and oxidative stress
- Nutrition, Genetics, and Disease
- Iron Metabolism and Disorders
- Cancer, Lipids, and Metabolism
- Rabbits: Nutrition, Reproduction, Health
- Animal Nutrition and Physiology
- Chromium effects and bioremediation
- Meat and Animal Product Quality
- Hippo pathway signaling and YAP/TAZ
- Escherichia coli research studies
- Hydrogen's biological and therapeutic effects
- Molecular Biology Techniques and Applications
- Consumer Attitudes and Food Labeling
- Advanced Chemical Sensor Technologies
- Cytomegalovirus and herpesvirus research
- Heme Oxygenase-1 and Carbon Monoxide
- Vitamin C and Antioxidants Research
- Cellular Mechanics and Interactions
- Cancer Cells and Metastasis
Université de Bordeaux
2020-2024
Centre National de la Recherche Scientifique
2021-2024
Bordeaux Population Health
2020-2024
Institut de Biochimie et Génétique Cellulaires
2024
Inserm
2020-2024
Karolinska Institutet
2016-2021
Institut Gustave Roussy
2021
Translational Research in Oncology
2020
Toxalim Research Centre in Food Toxicology
2012-2019
Association pour le Developpement de l'Institut de la Viande
2015-2019
Typhoid toxin-expressing
The World Health Organization classified processed and red meat consumption as "carcinogenic" "probably carcinogenic", respectively, to humans. Haem iron from plays a role in the promotion of colorectal cancer rodent models, association with enhanced luminal lipoperoxidation subsequent formation aldehydes. Here, we investigated short-term effects this haem-induced on mucosal gut homeostasis including microbiome F344 male rats fed haem-enriched diet (1.5 μmol/g) 14–21 days. Changes...
Abstract Gastric cancer’s (GC) bad prognosis is usually associated with metastatic spread. Invasive cancer stem cells (CSC) are considered to be the seed of GC metastasis and not all CSCs able initiate metastasis. Targeting these aggressive metastasis-initiating CSC (MIC) thus vital. Leukaemia inhibitory factor (LIF) hereby used target Hippo pathway oncogenic members, found induced in features. LIF-treated cell lines, patient-derived xenograft (PDX) and/or tumourspheres underwent...
Helicobacter pylori infection, the main risk factor for gastric cancer (GC), leads to an epithelial–mesenchymal transition (EMT) of epithelium contributing stem cell (CSC) emergence. The Hippo pathway effectors yes-associated protein (YAP) and transcriptional co-activator with PDZ binding motif (TAZ) control initiation progression in many cancers including GC. Here, we investigated role TAZ early steps H. pylori-mediated carcinogenesis. implication EMT, invasion, CSC-related tumorigenic...
Cancer stem cells (CSCs) present chemo-resistance mechanisms contributing to tumour maintenance and recurrence, making their targeting of utmost importance in gastric cancer (GC) therapy. The Hippo pathway has been implicated CSC properties was shown be regulated by leukaemia inhibitory factor receptor (LIFR) its ligand LIF breast cancer. This study aimed determine LIF’s effect on GC cell lines patient-derived xenograft (PDX) cells, which remains unexplored. treatment markers expression...
Several types of pathogenic bacteria produce genotoxins that induce DNA damage in host cells. Accumulating evidence suggests a central function these is to dysregulate the host's immune response, but underlying mechanisms remain unclear. To address this issue, we investigated effects most widely expressed bacterial genotoxin, cytolethal distending toxin (CDT), on T cells—the key mediators adaptive immunity. We show CDT induces premature senescence activated CD4 cells vitro and provide...
Epidemiological studies show that heme iron from red meat is associated with increased colorectal cancer risk. In carcinogen-induced-rats, a iron-rich diet increases the number of precancerous lesions and raises fecal biomarkers. Heme-induced lipoperoxidation measured by thiobarbituric acid reagents (TBARs) could explain promotion colon carcinogenesis heme. Using factorial design we studied if microbiota be involved in heme-induced carcinogenesis, modulating peroxidation. Rats treated or not...
Epidemiological studies have associated red meat intake with risk of colorectal cancer. Experimental explain this positive association by the oxidative properties heme iron released in colon. This latter is a potent catalyst for lipid peroxidation, resulting neoformation deleterious aldehydes fecal water heme-fed rats. The toxicity rats was to such peroxidation. study demonstrated that hemoglobin- and beef-fed preferentially induced apoptosis mouse normal colon epithelial cells than those...
Limiting the post-weaning intake of young rabbit is known to improve its resistance digestive disorders, whereas a degradation housing hygiene assumed have negative impact on health. This study aims at providing insights into mechanism health preservation regarding both host (growth and immune response) symbiotic microbiota. A 2×2 factorial design from weaning (day 28) day 64 was set up: ad libitum or restricted 70% libitum, high v. low (n=105 per group). At 36 45, 15 animals/group were...
Several commensal and pathogenic Gram-negative bacteria produce DNA-damaging toxins that are considered bona fide carcinogenic agents. The microbiota of colorectal cancer (CRC) patients is enriched in genotoxin-producing bacteria, but their role the pathogenesis CRC poorly understood. adenomatous polyposis coli (APC) gene mutated familial majority sporadic CRCs. We investigated whether loss APC alters response colonic epithelial cells to infection by Salmonella enterica, only bacterium...
Bacterial genotoxins cause DNA damage in eukaryotic cells, resulting activation of the response (DDR) vitro. These toxins are produced by Gram-negative bacteria, enriched microbiota inflammatory bowel disease (IBD) and colorectal cancer (CRC) patients. However, their role infection remains poorly characterized. We address typhoid toxin modulation host-microbial interaction health disease. Infection with a genotoxigenic Salmonella protects mice from intestinal inflammation. show that presence...
Red meat is probably carcinogenic to humans (WHO/IARC class 2A), in part through heme iron-induced lipoperoxidation. Here, we investigated whether red promotes carcinogenesis rodents and modulates associated biomarkers volunteers, speculating that an antioxidant marinade could suppress these effects via limitation of the induced lipid peroxidation. We gave marinated or non-marinated beef with various degrees cooking azoxymethane-initiated rats, Min mice, human volunteers (crossover study)....
In situ hybridization visualizes RNA in cells, but image analysis is complex. We present a protocol based on open-source software for automated high-content multiplex fluorescence transcriptomics analysis. Steps include nuclei segmentation with Fiji macro and quantification of up to 14 mRNA probes per image. describe procedures storing raw data, quality control images, the use Python app summarize all results one spreadsheet detailing number single or co-positive cells.
Gastric cancer, the fifth most common cancer worldwide, is mainly linked to Helicobacter pylori infection. H. induces chronic inflammation of gastric mucosa associated with high oxidative stress. Our study aimed at assessing implication Nrf2, a major regulator cellular redox homeostasis, in pylori-induced carcinogenesis.Using three different epithelial cell lines, non-cancerous (HFE-145) and two subtypes (AGS MKN74), we analyzed modulation Nrf2 expression over time. After invalidation by...
Summary In situ hybridization visualizes RNA in cells, but image analysis is complex. We present a protocol based on open-source software for automated high-content multiplex fluorescence transcriptomics analysis. Steps include nuclei segmentation with Fiji macro and quantification of up to 14 mRNA probes per image. describe procedures storing raw data, quality control images the use Python app summarize all results one spreadsheet detailing number single or co-positive cells.
Abstract BACKGROUND Glioblastoma (GB) is the most common subtype of glioma in adults. Despite treatment through tumor resection associated with chemo and radiotherapies, this cancer still has a very poor prognosis. Factors contributing to etiology, pathogenesis, or resistance are not well known. The significance understanding microbiome-gut-brain axis (MGBA) GB, topic that been recently shown be crucial several neurodegenerative diseases, cannot overstated. Therefore, our project aims...
We recently characterized the association between DNA damage and immunoresponse in vivo colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing genotoxin, known as typhoid toxin. In this protocol, we describe how to assess extent features infiltrating macrophages by double immunofluorescence. Total macrophage population was determined using an F4/80 antibody, whereas specific M2-like assessed CD206 antibody. For complete details on use execution please refer Martin et...