A5030945420- Phytochemicals and Antioxidant Activities
- Genomics, phytochemicals, and oxidative stress
- Diet and metabolism studies
- Tea Polyphenols and Effects
- Gut microbiota and health
- Free Radicals and Antioxidants
- High Altitude and Hypoxia
- Pancreatic function and diabetes
- Sirtuins and Resveratrol in Medicine
- Epilepsy research and treatment
- Trace Elements in Health
- Sulfur Compounds in Biology
- Metabolism and Genetic Disorders
- Biochemical Analysis and Sensing Techniques
- Diet, Metabolism, and Disease
- Cancer, Hypoxia, and Metabolism
- Antioxidant Activity and Oxidative Stress
- Neuroscience and Neuropharmacology Research
- Adipose Tissue and Metabolism
- Biochemical effects in animals
- Microbial Metabolites in Food Biotechnology
- Cancer, Lipids, and Metabolism
- Mitochondrial Function and Pathology
- Iron Metabolism and Disorders
- Genomics and Chromatin Dynamics
Catholic University of the Maule
2024
Griffith University
2017-2023
University of Chile
2010-2019
Universidad Bernardo O'Higgins
2019
Faculty (United Kingdom)
2019
The University of Queensland
2015-2017
Micron (United States)
2006
The aim of this study was to determine the protective effect quercetin, epigallocatechingallate, resveratrol, and rutin against disruption epithelial integrity induced by indomethacin in Caco-2 cell monolayers. Indomethacin decreased transepithelial electrical resistance increased permeability monolayers fluorescein-dextran. These alterations were abolished all tested polyphenols but rutin, with quercetin being most efficient. associated its capacity inhibit redistribution ZO-1 protein tight...
Studying rats fed high cholesterol diet and a pancreatic β-cell line (Min6), we aimed to determine the mechanisms by which quercetin protects against cholesterol-induced dysfunction impairments in glycemic control. Quercetin prevented increase total plasma cholesterol, but only partially diet-induced alterations lipid profile. decreases ATP levels mitochondrial bioenergetic Min6 cells, including membrane potentials coupling efficiency respiration (basal maximal oxygen consumption rate (OCR),...
KAT6A, and its paralog KAT6B, are histone lysine acetyltransferases (HAT) that acetylate H3K23 exert an oncogenic role in several tumor types including breast cancer where KAT6A is frequently amplified/overexpressed. However, pharmacologic targeting of to achieve therapeutic benefit has been a challenge. Here we describe identification highly potent, selective, orally bioavailable KAT6A/KAT6B inhibitor CTx-648 (PF-9363), derived from benzisoxazole series, which demonstrates anti-tumor...
OBJECTIVES: High-fat diets alter gut microbiota and barrier function, inducing metabolic endotoxemia low-grade inflammation. Whether these effects are due to the high dietary lipid content or concomitant decrease of carbohydrate intake is unclear. The aim this study was determine whether higher amounts fat reaching colon (through orlistat administration) affect colonic ecosystem in healthy volunteers effect prebiotic oligofructose (OF) model. METHODS: Forty-one young subjects were...
Abstract The nuclear factor erythroid 2‐related 2 pathway (Nrf2) has been previously identified to protect the brain against various impacts. Here, we investigated effect of Nrf2 activator sulforaphane in seizure models and hippocampal mitochondrial bioenergetics. We found that daily injections for 5 days elevated thresholds 6 Hz stimulation fluorothyl‐, but not pentylenetetrazole‐induced tonic seizures protected mice pilocarpine‐induced status epilepticus ( SE ). Also, increased antioxidant...
The protective effect of proanthocyanidin-containing polyphenol extracts from apples, avocados, cranberries, grapes, or proanthocyanidin microbial metabolites was evaluated in colonic epithelial cells exposed to p-cresol, a deleterious compound produced by the microbiota l-tyrosine. In HT29 Glc(-/+) cells, p-cresol significantly increased LDH leakage and decreased ATP contents, whereas Caco-2 cell monolayers, it transepithelial electrical resistance paracellular transport FITC-dextran....
Quercetin oxidation is generally believed to ultimately result in the loss of its antioxidant properties. To test this assertion, quercetin was induced, and after each major metabolites identified isolated by HPLC-DAD-ESI-MS/MS, (dichlorodihydrofluorescein oxidation-inhibiting) cytoprotective (LDH leakage-preventing) properties were evaluated Hs68 Caco2 cells exposed indomethacin. Compared quercetin, whole mixture (QOX) displayed a 20-fold greater potency. After resolution QOX into 12 peaks,...
The hypothesis that chronic feeding of the triglycerides octanoate (trioctanoin) and decanoate (tridecanoin) in "a regular non-ketogenic diet" is anticonvulsant was tested possible mechanisms actions were subsequently investigated. Chronic 35E% calories from tridecanoin, but not trioctanoin, reproducibly two acute CD1 mouse seizure models. levels beta-hydroxybutyrate plasma brain significantly increased by either treatment relative to control diet. respective are 76 µM 33 1.17 2.88 nmol/g...
Alterations in cardiac energy metabolism play a key role the pathogenesis of diabetic cardiomyopathy. Hypercholesterolemia associated with bioenergetic impairment and oxidative stress has not been well characterized function under glycemic control deficiency conditions. This work aimed to determine cardioprotective effects quercetin (QUE) against damage induced by high-cholesterol (HC) diet hyperglycemic rats, addressing intracellular antioxidant mechanisms bioenergetics. Quercetin reduced...
The stability of an apple peel polyphenol extract (APPE) with powerful antioxidant activity was evaluated under acidic conditions in vitro, and its protective effect against gastrointestinal damage investigated rats treated indomethacin. APPE remained stable at pH 2.0 for 4 h. In indomethacin (40 mg/kg ig), the previous administration protected gastric, intestinal, colonic mucosa from oxidative stress by preventing increased malondialdehyde concentrations decreasing GSH/GSSG ratio. also...
Temporal lobe epilepsy is a common form of adult and shows high resistance to treatment. Increasing evidence has suggested that metabolic dysfunction contributes the development seizures, with previous studies indicating impairments in brain glucose metabolism. Here we aim elucidate which pathways involved metabolism are impaired, by tracing hippocampal injected [U-13C]glucose (i.p.) during chronic stage pilocarpine-status epilepticus mouse model epilepsy. The enrichment 13C intermediates...
Chronic hypobaric hypoxia during fetal and neonatal life induces pulmonary hypertension. Hypoxia oxidative stress are driving this condition, which implies an increase generation of reactive oxygen species (ROS) and/or decreased antioxidant capacity. Melatonin has properties that decrease improves vascular function when administered postnatally. However, the effects antenatal treatment with melatonin in status unknown. Therefore, we hypothesized therapy arterial pressure high altitude...