A5018462821- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Ion channel regulation and function
- Exercise and Physiological Responses
- Pancreatic function and diabetes
- Adipokines, Inflammation, and Metabolic Diseases
- Mitochondrial Function and Pathology
- Muscle Physiology and Disorders
- Inflammasome and immune disorders
- Caveolin-1 and cellular processes
- Hormonal and reproductive studies
- Diabetes and associated disorders
- Calcium signaling and nucleotide metabolism
- Diet, Metabolism, and Disease
- Fibroblast Growth Factor Research
- Erythrocyte Function and Pathophysiology
- Liver Disease Diagnosis and Treatment
- Cardiovascular Disease and Adiposity
- Neonatal Respiratory Health Research
- Cholesterol and Lipid Metabolism
- Prostate Cancer Treatment and Research
- Pregnancy and preeclampsia studies
- Biochemical effects in animals
- Nicotinic Acetylcholine Receptors Study
- Fatty Acid Research and Health
University of Chile
2015-2024
Advanced Center for Chronic Diseases
2020
Faculty (United Kingdom)
2015
Centro de Estudios Científicos
2013
Instituto de Neurociencia Biomédica
2006
University of Cincinnati
2005
Studying rats fed high cholesterol diet and a pancreatic β-cell line (Min6), we aimed to determine the mechanisms by which quercetin protects against cholesterol-induced dysfunction impairments in glycemic control. Quercetin prevented increase total plasma cholesterol, but only partially diet-induced alterations lipid profile. decreases ATP levels mitochondrial bioenergetic Min6 cells, including membrane potentials coupling efficiency respiration (basal maximal oxygen consumption rate (OCR),...
Aim: We hypothesize that both type-1 ryanodine receptor (RyR1) and IP3-receptor (IP3R) calcium channels are necessary for the mitochondrial Ca2+ increase caused by membrane depolarization induced potassium (or electrical stimulation) of single skeletal muscle fibers; this would couple fiber excitation to an in metabolic output from mitochondria (excitation-metabolism coupling). Methods: Mitochondria matrix cytoplasmic levels were evaluated fibers isolated flexor digitorium brevis using...
Glucose-stimulated insulin secretion (GSIS) from pancreatic β-cells requires an increase in intracellular free Ca2+ concentration ([Ca2+]). Glucose uptake into promotes influx and reactive oxygen species (ROS) generation. In other cell types, ROS jointly induce release mediated by ryanodine receptor (RyR) channels. Therefore, we explored here if RyR-mediated contributes to GSIS β-cell islets isolated male rats. Stimulatory glucose increased islet secretion, promoted generation dissociated...
Insulin signaling includes generation of low levels H2O2; however, its origin and contribution to insulin-stimulated glucose transport are unknown. We tested the impact H2O2 on insulin-dependent GLUT4 translocation in skeletal muscle cells. increased GLUT4myc translocation, an effect additive that insulin. The anti-oxidants N-acetyl L-cysteine Trolox, p47phox/NOX2 NADPH oxidase inhibitory peptide gp91-ds-tat or p47phox knockdown each reduced translocation. Importantly, suppressed production....
Skeletal muscle is described as an endocrine organ, constitutively or intermittently secreting bioactive molecules. The signaling pathways by which these molecules mediate changes in skeletal and regulate interorgan crosstalk are only partly understood. Lactate widely a molecule different cells, but the role of lactate mature has not been fully unveiled. aim this study was to determine on activation adult mouse muscle. Male mice were injected intraperitoneally with saline, tissues dissected...
Reactive oxygen species (ROS) participate as signaling molecules in response to exercise skeletal muscle. However, the source of ROS and molecular mechanisms involved these phenomena are still not completely understood. The aim this work was study role muscle NADPH oxidase isoform 2 (NOX2) physical BALB/c mice, pre-treated with a NOX2 inhibitor, apocynin, (3 mg/kg) or vehicle for 3 days, were swim-exercised 60 min. Phospho-p47(phox) levels significantly upregulated by flexor digitorum brevis...
Fibroblast growth factor 21 (FGF21) is a hormone involved in the regulation of lipid, glucose, and energy metabolism. Although it released mainly from liver, recent years has been shown that "myokine", synthesized skeletal muscles after exercise stress conditions through an Akt-dependent pathway secreted for mediating autocrine endocrine roles. To date, molecular mechanism pathophysiological FGF21 production muscle not totally understood. We have previously demonstrated membrane...
Insulin resistance is defined as a reduced ability of insulin to stimulate glucose utilization. C57BL/6 mice fed with high-fat diet (HFD) are model resistance. In skeletal muscle, hydrogen peroxide (H2O2) produced by NADPH oxidase 2 (NOX2) involved in signaling pathways triggered insulin. We evaluated oxidative status muscle fibers from insulin-resistant and control determining H2O2 generation (HyPer probe), reduced-to-oxidized glutathione ratio NOX2 expression. After eight weeks HFD,...
Insulin stimulates glucose uptake in adult skeletal muscle by promoting the translocation of GLUT4 transporters to transverse tubule (T-tubule) membranes, which have particularly high cholesterol levels. We investigated whether T-tubule content affects insulin-induced transport. Feeding mice a high-fat diet (HFD) for 8 wk increased 30% triad-enriched vesicular fractions from tissue compared with triads control mice. Additionally, isolated fibers (flexor digitorum brevis) HFD-fed showed 40%...
We evaluated the anti-steatotic effects of n-3 long-chain polyunsaturated fatty acids plus extra virgin olive oil in liver mice fed a high fat diet.
Abstract Background Testosterone regulates nutrient and energy balance to maintain protein synthesis metabolism in cardiomyocytes, but supraphysiological concentrations induce cardiac hypertrophy. Previously, we determined that testosterone increased glucose uptake—via AMP-activated kinase (AMPK)—after acute treatment cardiomyocytes. However, whether elevated uptake is involved long-term changes of or required during cardiomyocyte growth remained unknown. In this study, hypothesized...
Low-grade chronic inflammation plays a pivotal role in the pathogenesis of insulin resistance (IR), and skeletal muscle has central this condition. NLRP3 inflammasome activation pathways promote low-grade several tissues. However, direct link between IR not been reported. Here, we evaluated components their GLUT4 translocation impairment during IR. Male C57BL/6J mice were fed with normal control diet (NCD) or high-fat (HFD) for 8 weeks. The protein levels NLRP3, ASC, caspase-1, gasdermin-D...
Ventricular arrhythmias are a common cause of sudden cardiac death, and their occurrence is higher in obese subjects. Abnormal gating ryanodine receptors (RyR2), the calcium release channels sarcoplasmic reticulum, can produce ventricular arrhythmias. Since obesity promotes oxidative stress RyR2 redox-sensitive channels, we investigated whether activity was altered mice. Mice fed high fat diet (HFD) became after eight weeks exhibited significant increase Single isolated from hearts mice were...
Aims Pancreatic β-cells synthesize and release serotonin (5 hydroxytryptamine, 5HT); however, the role of 5HT receptors on glucose stimulated insulin secretion (GSIS) mechanisms mediating this function is not fully understood. The aims study were to determine expression profile in murine MIN6 examine effects pharmacological activation receptor Htr2b GSIS mitochondrial function. Materials Methods mRNA levels cells quantified by RT qPCR. was assessed response global serotonergic with or...