A5063685802- Pesticide Residue Analysis and Safety
- Agricultural safety and regulations
- Pesticide Exposure and Toxicity
- Effects and risks of endocrine disrupting chemicals
- Carcinogens and Genotoxicity Assessment
- Insect and Pesticide Research
- Per- and polyfluoroalkyl substances research
- Toxic Organic Pollutants Impact
- Pluripotent Stem Cells Research
- Animal testing and alternatives
- Pharmacogenetics and Drug Metabolism
- Drug-Induced Hepatotoxicity and Protection
- Environmental Toxicology and Ecotoxicology
- Birth, Development, and Health
- Computational Drug Discovery Methods
- Estrogen and related hormone effects
- Drug Transport and Resistance Mechanisms
- Retinoids in leukemia and cellular processes
- Plant Toxicity and Pharmacological Properties
- 3D Printing in Biomedical Research
- Microbial metabolism and enzyme function
- Animal Ecology and Behavior Studies
- Pesticide and Herbicide Environmental Studies
- Mycotoxins in Agriculture and Food
- Cancer Cells and Metastasis
Wageningen University & Research
2015-2024
European Food Safety Authority
2023-2024
KWR Water Research Institute
2018-2021
Joint Research Centre
2013-2017
Weatherford College
2011-2013
Pension Fund for Care and Well-Being
2009-2011
Radboud University Medical Center
2010
Radboud University Nijmegen
2010
At present, regulatory assessment of systemic toxicity is almost solely carried out using animal models. The European Commission's REACH legislation stimulates the use animal-free approaches to obtain information on chemicals. In vitro tests provide in concentration-response curves for specific target cells, whereas vivo dose-response are regularly used human risk assessment. present study shows an approach predict developmental by combining data and silico kinetic modeling. A...
The currently available techniques for the safety evaluation of candidate drugs are usually cost-intensive and time-consuming often insufficient to predict human relevant cardiotoxicity. purpose this study was develop an in vitro repeated exposure toxicity methodology allowing identification predictive genomics biomarkers functional relevance drug-induced cardiotoxicity induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). hiPSC-CMs were incubated with 156 nM doxorubicin, which...
Abstract Per- and polyfluoroalkyl substances (PFASs) are omnipresent in the environment, food chain, humans. Epidemiological studies have shown a positive association between serum levels of perfluorooctanoic acid (PFOA) perfluorooctane sulfonic (PFOS), increased cholesterol and, some cases, also triglyceride levels. However, causality has been questioned, as animal studies, well human trial, showed decrease no effects or plasma triglycerides. To obtain more insight into PFASs on these...
Abstract Acetamiprid is a pesticide active substance with insecticidal action whose approval was renewed by Commission Implementing Regulation (EU) 2018/113. In January 2022, the EFSA PPR Panel published statement following request from European to advise on human health or environment based new scientific evidence presented France during decision-making phase. July means of further mandate received Commission, requested provide advice if information and any other that has become available...
Abstract Studies in the field of molecular nutrition and food research often aim at identifying effects bioactive ingredients on living organisms. When data from human studies are difficult to obtain, studied relevant animal or cellular vitro models. This poses need for adequate extrapolation vivo situation, high‐dose levels realistic low‐dose experimental animals humans. Furthermore, genetic polymorphisms lifestyle factors may have be taken into account. Physiologically based kinetic (PBK)...
Pyrrolizidine alkaloids (PAs) are secondary metabolites from plants that have been found in substantial amounts herbal supplements, infusions and teas. Several PAs cause cancer animal bioassays, mediated via a genotoxic mode of action, but for the majority PAs, carcinogenicity data lacking. It is assumed risk assessment all same potency as riddelliine, which considered to be one most potent carcinogenic rats. This may overestimate risks, since many expected lower potencies. In this study we...
The goal of the present study was to develop an online web-based toolbox that contains generic physiologically based kinetic (PBK) models for rats and humans, including underlying calculation tools predict plasma protein binding tissue:plasma distribution, be used quantitative in-vitro-to-in-vivo extrapolations (QIVIVE). PBK within allow first estimations internal tissue concentrations chemicals made, on logP pKa values intestinal uptake intrinsic hepatic clearance. As a case study, oral...
The current study was conducted to assess the levels of organophosphates and carbamates in vegetables Kenya examine potential consumer health risks. A total 90 samples were analysed by liquid chromatography/high-resolution tandem mass spectrometry. Residues acephate, chlorpyrifos, methamidophos, omethoate profenofos found 22% samples, ranging from 10 1343 μg/kg. EU MRL exceeded 21%, 10%, 8% French beans, kales, spinach tomatoes, respectively. Chlorpyrifos had an acute HQ 3.3 2.2 for children...
Abstract Per- and polyfluoroalkyl substances (PFASs) are omnipresent have been shown to induce a wide range of adverse health effects, including hepatotoxicity, developmental toxicity, immunotoxicity. The aim the present work was assess whether human HepaRG liver cells can be used obtain insight into differences in hepatotoxic potencies series PFASs. Therefore, effects 18 PFASs on cellular triglyceride accumulation (AdipoRed assay) gene expression (DNA microarray for PFOS RT-qPCR all PFASs)...
The embryonic stem cell test (EST) has been proposed as an in vitro assay that might reduce animal experimentation regulatory developmental toxicology. So far, evaluation of the EST was not performed using compounds within distinct chemical classes. Evaluation a class chemically related can define usefulness for tested. aim present study to evaluate relative sensitivity selected series homologous and compare data toxicity vivo. To this end proximate developmentally toxic glycol ether alkoxy...
To obtain insight in translocation of nanoparticles across the placental barrier, was studied for one positively and two negatively charged polystyrene (PS-NPs) similar size an vitro model. The model consisted BeWo b30 cells, derived from a human choriocarcinoma grown on transwell insert forming cell layer that separates apical basolateral compartment. PS-NPs were characterized with respect to size, surface charge, morphology protein corona. Translocation not related PS-NP charge. Two...
Lasiocarpine and riddelliine are pyrrolizidine alkaloids (PAs) known to cause liver toxicity. The aim of this study was predict the inter-species inter-ethnic human differences in acute toxicity lasiocarpine using physiologically based kinetic (PBK) modelling reverse dosimetry vitro data. concentration–response curves cytotoxicity defined pooled hepatocytes were translated vivo dose–response by PBK models developed data obtained from incubations with tissue fractions Chinese Caucasian...
Abstract Poly- and perfluoroalkyl substances (PFASs) are omnipresent in the environment have been shown to accumulate humans. Most PFASs not biotransformed animals humans, so that elimination is largely dependent on non-metabolic clearance via bile urine. Accumulation of certain humans may relate their reabsorption from pre-urine by transporter proteins proximal tubules kidney, such as URAT1 OAT4. The present study assessed vitro transport 7 (PFHpA, PFOA, PFNA, PFDA, PFBS, PFHxS PFOS)...
Abstract The conclusions of the European Food Safety Authority (EFSA) following peer review initial risk assessments carried out by competent authorities rapporteur Member State, Belgium, and co‐rapporteur Austria, for pesticide active substance lenacil are reported. context was that required Commission Implementing Regulation (EU) No 844/2012, as amended 2018/1659. were reached on basis evaluation representative uses a herbicide sugar fodder beet (field use). reliable end points,...