A5006145213- Animal testing and alternatives
- Effects and risks of endocrine disrupting chemicals
- Pharmacogenetics and Drug Metabolism
- Carcinogens and Genotoxicity Assessment
- Drug Transport and Resistance Mechanisms
- Drug-Induced Hepatotoxicity and Protection
- Computational Drug Discovery Methods
- Immunotoxicology and immune responses
- Toxic Organic Pollutants Impact
- Liver physiology and pathology
- Pesticide Exposure and Toxicity
- Eicosanoids and Hypertension Pharmacology
- Consumer Attitudes and Food Labeling
- Dye analysis and toxicity
- 3D Printing in Biomedical Research
- Hemoglobin structure and function
- Genomics, phytochemicals, and oxidative stress
- Pharmacological Effects and Toxicity Studies
- Biomedical Ethics and Regulation
- Environmental Toxicology and Ecotoxicology
- Hormonal and reproductive studies
- Analytical Chemistry and Chromatography
- Viral Infectious Diseases and Gene Expression in Insects
- Antibiotics Pharmacokinetics and Efficacy
- Retinoids in leukemia and cellular processes
Utrecht University
2012-2021
Novozymes (Denmark)
2013
Institute for In Vitro Sciences
2013
Federal Institute for Risk Assessment
2013
World Health Organization
1998
National Institute of Environmental Health Sciences
1998
National Center for Toxicological Research
1998
Ain Shams University
1998
Hospital Universitari i Politècnic La Fe
1994
MRC Toxicology Unit
1979-1980
High content omic techniques in combination with stable human vitro cell culture systems have the potential to improve on current pre-clinical safety regimes by providing detailed mechanistic information of altered cellular processes. Here we investigated added benefit integrating transcriptomics, proteomics and metabolomics together pharmacokinetics for drug testing regimes. Cultured renal epithelial cells (RPTEC/TERT1) were exposed nephrotoxin Cyclosporine A (CsA) at therapeutic...
The introduction of in vitro methodologies the toxicological risk assessment process requires a number prerequisites regarding both toxicodynamics and biokinetics compounds under study. In systems will need to be relevant for measuring those structural physiological changes that are good indicators adverse effects. Furthermore, dose metric found have an effect system should relevant. One element defining appropriate is related kinetic behavior compound system: binding proteins, plastic,...
At present, regulatory assessment of systemic toxicity is almost solely carried out using animal models. The European Commission's REACH legislation stimulates the use animal-free approaches to obtain information on chemicals. In vitro tests provide in concentration-response curves for specific target cells, whereas vivo dose-response are regularly used human risk assessment. present study shows an approach predict developmental by combining data and silico kinetic modeling. A...
Difficulties may arise when extrapolating in vitro derived toxicity data to vivo because of the high variability and occasional low sensitivity results. Differences free concentration a test compound between systems different part explain this difference. The aim study was determine what assay components influence phenanthrene Balb/c 3T3 RTgill-W1 MTT assay. Partition coefficients serum, well plate plastic, cells, headspace were measured subsequently used model vitro. estimated compared...
The role that in vitro systems can play toxicological risk assessment is determined by the appropriateness of chosen methods, with respect to way which data be extrapolated vivo situation. This report presents results a workshop aimed at better defining use vitro-derived biomarkers toxicity (BoT) and determining place these have human assessment. As result, conceptual framework presented for incorporation into process. selection BoT takes account they need distinguish adverse adaptive...
Solid phase microextraction (SPME) is an extraction technique that uses a polymer-coated fiber as the device. After extraction, compound of interest can be desorbed from and subsequently analyzed by GC or HPLC. One properties SPME only freely dissolved fraction chemical available for partitioning to The method applied in way small amounts are extracted sample, which allows negligible depletion extraction. These two make devices particularly suitable measurements free concentrations. In...
Human hepatic glutathione S-transferase (GST) subunits were characterized and quantified with the aid of a recently developed h.p.l.c. method. In 20 tissue specimens absolute amounts basic Class Alpha B1 B2, near-neutral Mu mu psi acidic subunit pi determined. The average total amount GST was 37 micrograms/mg cytosolic protein, being predominant class (84% GSTs), as sole representative Pi GSTs present in lowest concentration (4% GSTs). Large interindividual differences observed for all...