A5016723501- Nanoparticles: synthesis and applications
- Microplastics and Plastic Pollution
- 3D Printing in Biomedical Research
- Heavy Metal Exposure and Toxicity
- Microfluidic and Capillary Electrophoresis Applications
- Effects and risks of endocrine disrupting chemicals
- Recycling and Waste Management Techniques
- Drug Transport and Resistance Mechanisms
- Innovative Microfluidic and Catalytic Techniques Innovation
- Healthcare and Environmental Waste Management
- Graphene and Nanomaterials Applications
- Occupational and environmental lung diseases
- Nanoparticle-Based Drug Delivery
- Computational Drug Discovery Methods
- Electrochemical Analysis and Applications
- Aluminum toxicity and tolerance in plants and animals
- Mycotoxins in Agriculture and Food
- Environmental Toxicology and Ecotoxicology
- Air Quality and Health Impacts
- Biotechnology and Related Fields
- Nanotechnology research and applications
- Pesticide Residue Analysis and Safety
- Neuroscience and Neuropharmacology Research
- Pesticide Exposure and Toxicity
- Risk and Safety Analysis
Wageningen University & Research
2015-2024
Utrecht University
2001-2006
University Medical Center Utrecht
2004-2006
Northwestern University
1999
We report the results of a 28-day oral exposure study in rats, exposed to <20 nm noncoated, or <15 PVP-coated silver nanoparticles ([Ag] = 90 mg/kg body weight (bw)), AgNO(3) 9 bw), carrier solution only. Dissection was performed at day 29, and after wash-out period 1 8 weeks. Silver present all examined organs with highest levels liver spleen for treatments. concentrations were highly correlated amount Ag(+) nanoparticle suspension, indicating that mainly Ag(+), much lesser extent...
This study uniquely describes all steps of the risk assessment process for use one specific nanomaterial (nanosilica) in food products. The aim was to identify gaps essential knowledge and difficulties uncertainties associated with each these steps. Several products added silica (E551) were analyzed presence, particle size concentration nanosilica particles, using experimental analytical data, intake via estimated. As no information is available on absorption from gastrointestinal tract, two...
The hippocampus is believed to be an important structure for learning tasks that require temporal processing of information. trace classical conditioning paradigm requires because the conditioned stimulus (CS) and unconditioned (US) are temporally separated by empty interval. present study sought determine whether was necessary rats perform a fear task in which each 10 trials consisted auditory tone CS (15-s duration) followed 30-s interval then fear-producing floor-shock US (0.5-s...
Titanium dioxide (TiO2) is a common food additive used to enhance the white color, brightness, and sometimes flavor of variety products. In this study 7 grade TiO2 materials (E171), 24 products, 3 personal care products were investigated for their content number-based size distribution particles present in these Three principally different methods have been determine particles: electron microscopy, asymmetric flow field-flow fractionation combined with inductively coupled mass spectrometry,...
Nanotechnology has the potential to innovate agricultural, feed and food sectors (hereinafter referred as agri/feed/food). Applications that are marketed already include nano-encapsulated agrochemicals or nutrients, antimicrobial nanoparticles active intelligent packaging. Many nano-enabled products currently under research development, may enter market in near future. As for any other regulated product, applicants applying approval have demonstrate safe use of such new without posing undue...
The presence, dissolution, agglomeration state, and release of materials in the nano-size range from food containing engineered nanoparticles during human digestion is a key question for safety assessment these materials. We used an vitro model to mimic digestion. Food products subjected included (i) hot water, (ii) coffee with powdered creamer, (iii) instant soup, (iv) pancake which either contained silica as additive E551, or form synthetic amorphous 32 nm SiO2 particles were added....
spICP-MS measurement with a powerful data evaluation tool is presented as fast, cost efficient and easy to use screening for metal oxide NPs in complex matrices.
Oral ingestion is an important exposure route for silver nanoparticles (AgNPs), but their fate during gastrointestinal digestion unknown. This was studied 60 nm AgNPs and ions (AgNO₃) using in vitro human model. Samples after saliva, gastric intestinal were analysed with SP-ICPMS, DLS SEM-EDX. In presence of proteins, the number particles dropped significantly, to rise back original values digestion. SEM-EDX revealed that reduction caused by clustering. These clusters composed chlorine....
Synthetic Amorphous Silica (SAS) is commonly used in food and drugs. Recently, a consumer intake of silica from was estimated at 9.4 mg/kg bw/day, which 1.8 bw/day to be the nano-size range. Food products containing SAS have been shown contain nanometer size range (i.e. 5 – 200 nm) up 43% total content. Concerns raised about possible adverse effects chronic exposure nanostructured silica. Rats were orally exposed 100, 1000 or 2500 SAS, 500 NM-202 (a representative for OECD testing) 28 days,...
Abstract To assess the potential risk of microplastic exposure to humans and aquatic ecosystems, reliable toxicity data is needed. This includes a more complete foundational understanding better characterization hazards they may present. expand this understanding, an international group experts was convened in 2020–2021 identify critical thresholds at which microplastics found drinking ambient waters present health organisms. However, their findings were limited by notable gaps literature....
Abstract Background Micro- and nanoplastics (MNPs) represent one of the most widespread environmental pollutants twenty-first century to which all humans are orally exposed. Upon ingestion, MNPs pass harsh biochemical conditions within gastrointestinal tract, causing a unique protein corona on MNP surface. Little is known about digestion-associated its impact cellular uptake MNPs. Here, we systematically studied influence digestion neutral charged polystyrene using THP-1-derived macrophages....
The mesencephalic dopamine (mesDA) system is involved in the control of movement and behavior. expression Pitx3 brain restricted to mesDA gene induced relatively late, at E11.5, a time when tyrosine hydroxylase (Th) initiated. We show here that, Pitx3-deficient aphakia(ak) mouse mutant, malformed. Owing developmental failure neurons lateral field midbrain,mesDA are not found SNc projections caudate putamen selectively lost. However, expressed all animals. Therefore, react specifically loss...
Applications of nanoparticles in the food sector are eminent. Silver among most frequently used, making consumer exposure to silver inevitable. Information about uptake through intestines and possible toxic effects is therefore very important but still lacking. In present study, we used an vitro model for human intestinal epithelium consisting Caco-2 M-cells study passage their ionic equivalents assess on whole-genome mRNA expression. This intestine was exposed four sizes 4 h. Exposure ions...
Intestinal translocation is a key factor for determining bioavailability of nanoparticles (NPs) after oral uptake. Therefore, we evaluated three in vitro intestinal cell models increasing complexity which might affect the NPs: mono-culture (Caco-2 cells), co-culture with mucus secreting HT29-MTX cells and tri-culture M-cells. Cell were exposed to well characterized differently sized (50 100 nm) charged (neutral, positively negatively) polystyrene NPs. In addition, two types negatively NPs...
This paper presents the outcomes from a workshop of European Network on Health and Environmental Impact Nanomaterials (NanoImpactNet). During workshop, 45 experts in field safety assessment engineered nanomaterials addressed need to systematically study sets with specific metrics generate data set which would allow establishment dose-response relations. The group concluded that international cooperation worldwide standardization terminology, reference materials protocols are needed make...
The likelihood of oral exposure to nanoparticles (NPs) is increasing, and it necessary evaluate the bioavailability NPs. In vitro approaches could help reducing animal studies, but validation against in vivo studies essential. Previously, we assessed translocation 50 nm polystyrene NPs different charges (neutral, positive negative) using a Caco-2/HT29-MTX intestinal model. translocated surface charge-dependent manner. present study aimed validate this model by an study. For this, rats were...
Titanium dioxide (TiO2) is produced at high volumes and applied in many consumer food products. Recent toxicokinetic modelling indicated the potential of TiO2 to accumulate human liver spleen upon daily oral exposure, which not routinely investigated chronic animal studies. A health risk from nanosized particle consumption could be excluded then. Here we show first quantification both total titanium (Ti) particles 15 post-mortem livers spleens. These low-level analyses were enabled by use...