A5054309171- Environmental Toxicology and Ecotoxicology
- Pharmaceutical and Antibiotic Environmental Impacts
- Effects and risks of endocrine disrupting chemicals
- Reproductive biology and impacts on aquatic species
- Toxic Organic Pollutants Impact
- Fish Ecology and Management Studies
- Microplastics and Plastic Pollution
- Recycling and Waste Management Techniques
- Molecular Biology Techniques and Applications
- bioluminescence and chemiluminescence research
- Water Quality and Pollution Assessment
- Gene expression and cancer classification
- Aquaculture disease management and microbiota
- Pesticide Exposure and Toxicity
- Epigenetics and DNA Methylation
- Mercury impact and mitigation studies
- Environmental Chemistry and Analysis
- Ethics in Clinical Research
- Environmental DNA in Biodiversity Studies
- Heavy Metal Exposure and Toxicity
- Nutrition and Health in Aging
- Water Quality Monitoring and Analysis
- Ethics and Legal Issues in Pediatric Healthcare
- Estrogen and related hormone effects
- Microbial bioremediation and biosurfactants
Southern California Coastal Water Research Project
2013-2024
University of Florida
2011-2020
Middle East Studies Association of North America
2017
University of Sussex
2010
University of Exeter
2010
Thousands of organic micropollutants and their transformation products occur in water. Although often present at low concentrations, individual compounds contribute to mixture effects. Cell-based bioassays that target health-relevant biological endpoints may therefore complement chemical analysis for water quality assessment. The objective this study was evaluate cell-based suitability benchmark assess efficacy treatment processes. selected cover relevant steps the toxicity pathways...
Diclofenac, a nonsteroidal anti-inflammatory drug, is widely detected in surface waters and can potentially cause deleterious effects fish. Here, we investigated the biological of 21-day exposure to waterborne diclofenac at environmentally relevant concentrations (0, 0.5, 1, 5, 25 μg/L) rainbow trout. Accumulation bile was measured responses selected tissues were assessed via changes expression genes (cytochrome P450 (cyp) 1a1, cyclooxygenase (cox) 1 2, p53) involved metabolism xenobiotics,...
Abstract Microplastic particles (MPs) are ubiquitous across a wide range of aquatic habitats but determining an appropriate level risk management is hindered by poor understanding environmental risk. Here, we introduce framework for ecosystems that identifies four critical thresholds, ranging from low regulatory concern to the highest where pollution control measures could be introduced mitigate emissions. The thresholds were derived using species sensitivity distribution (SSD) approach and...
Abstract To assess the potential risk of microplastic exposure to humans and aquatic ecosystems, reliable toxicity data is needed. This includes a more complete foundational understanding better characterization hazards they may present. expand this understanding, an international group experts was convened in 2020–2021 identify critical thresholds at which microplastics found drinking ambient waters present health organisms. However, their findings were limited by notable gaps literature....
Abstract Throughout the past decade, many studies have reported adverse effects in biota following microplastic exposure. Yet, field is still emerging as current understanding of toxicity limited. At same time, recent legislative mandates required environmental regulators to devise strategies mitigate pollution and develop health-based thresholds for protection human ecosystem health. The publication rate also presents a unique challenge scientists, managers, other communities may find it...
Many pollutants cause endocrine disruption in aquatic organisms. While studies of the direct effects toxicants on exposed organisms are commonplace, little is known about potential for toxicant exposures a parental (F0) generation to affect unexposed F1 or F2 generations (multigenerational and transgenerational effects, respectively), particularly estuarine fishes. To investigate this possibility, we inland silversides (Menidia beryllina) environmentally relevant (low ng/L) concentrations...
The inland silverside, Menidia beryllina, is a euryhaline fish and model organism in ecotoxicology. We previously showend that exposure to picomolar (ng/L) levels of endocrine disrupting chemicals (EDCs) can cause variety effects M. from changes gene expression phenotypic alterations. Here we explore the potential for early life EDCs modify epigenome silversides, with focus on multi- transgenerational effects. included contaminants emerging concern (the pyrethroid insecticide bifenthrin...
The new technologies for next-generation sequencing (NGS) and global gene expression analyses that are widely used in molecular medicine increasingly applied to the field of fish biology. This has facilitated directions address research areas could not be previously considered due lack information ecologically relevant species. Over past decade, cost NGS decreased significantly, making it possible use non-model species investigate emerging environmental issues. have permitted researchers...
The present study investigated whether a combination of targeted analytical chemistry information with unsupervised, data-rich biological methodology (i.e., transcriptomics) could be utilized to evaluate relative contributions wastewater treatment plant (WWTP) effluents effects. effects WWTP on fish exposed ambient, receiving waters were studied at three locations distinct and watershed characteristics. At each location, 4 d exposures male fathead minnows the effluent upstream downstream...
The chemical-specific risk-based paradigm that informs monitoring and assessment of environmental contaminants does not apply well to the many thousands new chemicals are being introduced into ambient receiving waters. We propose a tiered framework incorporates bioanalytical screening tools diagnostic nontargeted chemical analysis more effectively monitor for emerging concern (CECs). is based on comprehensive battery in vitro bioassays first screen broad spectrum CECs analytical methods...
The presence of numerous chemical contaminants from industrial, agricultural, and pharmaceutical sources in water supplies poses a potential risk to human ecological health. Current analyses suffer limitations, including coverage high cost, broad-coverage vitro assays such as transcriptomics may further improve quality monitoring by assessing large range possible effects. Here, we used high-throughput assess the activity induced field-derived extracts MCF7 breast carcinoma cells. Wastewater...
This study aimed to evaluate the utility of microarrays as a biomonitoring tool in field studies. A 15,000-oligonucleotide microarray was used measure hepatic gene expression fathead minnows (Pimephales promelas) caged four Nebraska, USA watersheds - Niobrara and Dismal Rivers (low-impact agricultural sites) Platte Elkhorn (high-impact sites). Gene profiles were site specific fish from low- high-impact sites aggregated into distinct groups. Over 1500 genes differentially regulated between...
Cyclooxygenase (COX) inhibitors are ubiquitous in aquatic systems and have been detected fish tissues. The exposure of to these pharmaceuticals is concerning because COX disrupt the synthesis prostaglandins (PGs), which modulate a variety essential biological functions, including reproduction. In this study, we investigated effects well-characterized mammalian on female fathead minnow reproductive health. Fish (n = 8) were exposed for 96 h water containing indomethacin (IN; 100 µg/l),...
In vitro transactivation bioassays have shown promise as water quality monitoring tools, however their adoption and widespread application has been hindered partly due to a lack of standardized methods availability robust, user-friendly technology. this study, commercially available, division-arrested cell lines were employed quantitatively screen for endocrine activity chemicals present in samples interest environmental professionals. A single, protocol that included comprehensive...
Abstract Acute environmental stressors such as short-term exposure to pollutants can have lasting effects on organisms, potentially impacting future generations. Parental toxicants result in changes the epigenome (e.g., DNA methylation) that are passed down subsequent, unexposed However, it is difficult gauge cumulative population-scale impacts of epigenetic from laboratory experiments alone. Here, we developed a size- and age-structured delay-coordinate population model evaluate long-term...
Abstract High‐throughput cell assays that detect and integrate the response of multiple chemicals acting via a common mode action have potential to enhance current environmental monitoring practices. Establishing linkage between in vitro vivo responses is key demonstrating can be predictive ecologically relevant outcomes. The present study investigated potency 17β‐estradiol (E2), estrone (E1), nonylphenol (NP), treated wastewater effluent using readily available GeneBLAzer ® estrogen...
The impact of unmonitored contaminants, also known as contaminants emerging concern (CECs), on freshwater streams remains largely uncharacterized. Water samples from 31 representing urban, agricultural and undeveloped (i.e., open space) land use in Southern California (USA) were analyzed for vitro vivo bioactivity. extent magnitude bioactivity screened using endocrine-responsive cell bioassays a fish embryo screening assay low. In contrast, wider gradient responses the aryl hydrocarbon...
To broaden the scope of contaminants monitored in human-impacted riverine systems, water, sediment, and treated wastewater effluent were analyzed using receptor-based cell assays that provide an integrated response to chemicals based on their mode biological activity. Samples collected from three California (USA) watersheds with varying degrees urbanization discharge municipal treatment plants (WWTPs). complement assay results, samples also for a suite emerging concern (CECs) gas liquid...