A5089207531- Effects and risks of endocrine disrupting chemicals
- Environmental Toxicology and Ecotoxicology
- Reproductive biology and impacts on aquatic species
- Carcinogens and Genotoxicity Assessment
- Pluripotent Stem Cells Research
- Marine Biology and Environmental Chemistry
- Sperm and Testicular Function
- Renal and related cancers
- Aquaculture disease management and microbiota
- Sexual Differentiation and Disorders
- Retinoids in leukemia and cellular processes
- Aquaculture Nutrition and Growth
- Molecular Biology Techniques and Applications
- Hormonal and reproductive studies
- Pesticide and Herbicide Environmental Studies
- Lipid Membrane Structure and Behavior
- Cancer, Lipids, and Metabolism
- Prostate Cancer Treatment and Research
- Insect and Pesticide Research
- Drug Transport and Resistance Mechanisms
- Marine Ecology and Invasive Species
- Prostate Cancer Diagnosis and Treatment
- Birth, Development, and Health
- Advanced biosensing and bioanalysis techniques
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Brown University
2013-2022
University of Nottingham
2021
Iowa State University
2021
University of Florida
2010-2013
Abstract Maintaining the viability of populations plants and animals is a key focus for environmental regulation. Population‐level responses integrate cumulative effects chemical stressors on individuals as those interact with are affected by their conspecifics, competitors, predators, prey, habitat, other biotic abiotic factors. Models population‐level contaminants can information from lower levels biological organization feed that into higher‐level community ecosystem models. As...
The new technologies for next-generation sequencing (NGS) and global gene expression analyses that are widely used in molecular medicine increasingly applied to the field of fish biology. This has facilitated directions address research areas could not be previously considered due lack information ecologically relevant species. Over past decade, cost NGS decreased significantly, making it possible use non-model species investigate emerging environmental issues. have permitted researchers...
In utero exposure to antiandrogenic xenobiotics such as di-n-butyl phthalate (DBP) has been linked congenital defects of the male reproductive tract, including cryptorchidism and hypospadias, well later life effects testicular cancer decreased sperm counts. Experimental evidence indicates that DBP in rat. However, it is unclear whether similar on androgen biosynthesis human fetal testis. To address this issue, we developed a xenograft bioassay with multiple androgen-sensitive physiological...
Background Queen conch (Strombus gigas) reproduction is inhibited in nearshore areas of the Florida Keys, relative to offshore environment where conchs reproduce successfully. Nearshore reproductive failure possibly a result exposure environmental factors, including heavy metals, which are likely accumulate close shore. Metals such as Cu and Zn detrimental many mollusks. Methodology/Principal Findings Histology shows gonadal atrophy compared reproductively healthy conchs. In order determine...
In utero exposure to some phthalate esters adversely affects the development of rat seminiferous cord, causing germ cell loss and increasing number multinucleated cells (MNGs). To understand timing MNG formation determine whether it requires nuclear division, timed pregnant Sprague Dawley rats were exposed 500 mg/kg di-n-butyl (DBP) or corn oil vehicle by oral gavage on Gestational Day (GD) 17 18 (0 h) euthanized after 2, 4, 6, 24 h given a second dose at 48 initial dose. Dams simultaneously...
Abstract BACKGROUND Prostate cancer is the most commonly diagnosed nonskin in men. The etiology of prostate unknown, although both animal and epidemiologic data suggest that early life exposures to various toxicants, may impact DNA methylation status during development, playing an important role. METHODS We have developed a xenograft model characterize growth differentiation human fetal implants (gestational age 12–24 weeks) can provide new on potential role stressors cancer. expression key...
Summary The tumor suppressor protein p53 ( TP 53) has many functions in cell cycle regulation, apoptosis, and DNA damage repair is also involved spermatogenesis the mouse. To evaluate role of rat, we characterized testis biology adult males a novel knockout rat SD ‐ Tp53 tm1sage ). rats exhibited variable levels testicular atrophy, including significantly decreased weights, atrophic seminiferous tubules, tubule diameter, elevated spermatocyte TUNEL labeling rates, indicating dysfunction...
Humans are universally exposed to low levels of phthalate esters (phthalates), which used plasticize polyvinyl chloride. Phthalates exert adverse effects on the development seminiferous cords in fetal testis through unknown toxicity pathways. To investigate hypothesis that phthalates alter cord by disrupting retinoic acid (RA) signaling testis, gestational day 15 rat testes were for 1–3 days 10−6 M all-trans (ATRA) alone or combination with 10−6–10−4 mono-(2-ethylhexyl) (MEHP) ex vivo...
Exposure to excess retinoic acid (RA) disrupts the development of mammalian testicular seminiferous cord.However, molecular events surrounding RA-driven loss cord structure have not previously been examined.To investigate mechanisms associated with this adverse developmental effect, fetal rat testes were isolated on gestational day 15, after testis determination and initiation development, cultured in media containing all-trans RA (ATRA; 10 28 26 M) or vehicle for 3 days.ATRA exposure...
Phthalic acid esters (phthalates) are a class of industrial chemicals that cause developmental and reproductive toxicity, but there significant gaps in knowledge phthalate toxicity mechanisms. There is evidence phthalates disrupt retinoic signaling the fetal testis, potentially disrupting control spatial temporal patterns testis development. Our goal was to determine how would interact with during mouse We hypothesized mono-(2-ethylhexyl) (MEHP) exacerbate adverse effect all-trans (ATRA) on...
Phthalic acid esters (phthalates) are male reproductive toxicants, which exert their most potent toxicity during fetal development. In the rat, exposure to phthalates reduces testosterone biosynthesis, alters development of seminiferous cords and other tissues, induces formation abnormal multinucleated germ cells (MNGs). Identification MNGs is a time-intensive process, it requires specialized training identify in histological sections. As result, not routinely quantified phthalate...
Abstract Phthalic acid esters (phthalates) are male reproductive toxicants, which exert their most potent toxicity during a critical window of sensitivity in fetal development. In the rat, exposure to phthalates reduces testosterone biosynthesis, alters development seminiferous cords and other tissues, induces formation abnormal multinucleated germ cells (MNGs). Identification MNGs is time-intensive process, it requires specialized training identify histological sections. As result, not...