A5088340387- Reproductive biology and impacts on aquatic species
- Environmental Toxicology and Ecotoxicology
- Viral-associated cancers and disorders
- Cytomegalovirus and herpesvirus research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cancer-related molecular mechanisms research
- Aquaculture Nutrition and Growth
- Fish Ecology and Management Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Nanoparticles: synthesis and applications
- Immune Cell Function and Interaction
- Sperm and Testicular Function
- Sex and Gender in Healthcare
- Herpesvirus Infections and Treatments
- Effects and risks of endocrine disrupting chemicals
- Gene expression and cancer classification
- Potato Plant Research
- Physiological and biochemical adaptations
- Electrochemical Analysis and Applications
- Pesticide Exposure and Toxicity
- Arsenic contamination and mitigation
- Marine Biology and Environmental Chemistry
- Estrogen and related hormone effects
- Retinoids in leukemia and cellular processes
- Molecular Biology Techniques and Applications
University of Florida Health
2019-2024
University of Florida
2010-2024
University of New Brunswick
2013-2020
The objective of this study was to evaluate the distribution silver nanoparticles (NPs) in pregnant mice and their developing embryos. Silver NPs (average diameter 50 nm) were intravenously injected into CD-1 on gestation days (GDs) 7, 8, 9 at dose levels 0, 35, or 66 μg Ag/mouse. Mice euthanised GD10, tissue samples collected analysed for content. Compared with control animals citrate buffer vehicle, content significantly increased (p < 0.05) nearly all tissues from NP-treated mice....
Abstract Kaposi's sarcoma-associated herpesvirus is the etiologic agent of sarcoma and two B-cell malignancies. Recent advancements in sequencing technologies have led to high resolution transcriptomes for several human herpesviruses that densely encode genes on both strands. However, KSHV progress remained limited due overall low percentage transcripts, even during lytic replication. To address this challenge, we developed a target enrichment method increase KSHV-specific reads short-...
The gammaherpesviruses, including Epstein-Barr virus (EBV), Kaposi's sarcoma-associated herpesvirus (KSHV), and murine gammaherpesvirus 68 (MHV68, MuHV-4, γHV68), are etiologic agents of a wide range lymphomas non-hematological malignancies. These viruses possess large highly dense dsDNA genomes that feature >80 bidirectionally positioned open reading frames (ORFs). abundance overlapping transcripts extensive splicing throughout these have until now prohibited high throughput-based...
Gammaherpesviruses, including the human pathogens Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV), establish lifelong latent infection in B cells are associated with a variety of tumors. In addition to protein coding genes, these viruses encode numerous microRNAs (miRNAs) within their genomes. While putative host targets EBV KSHV miRNAs have been previously identified, specific functions during vivo largely unknown. Murine gammaherpesvirus 68 (MHV68) is natural...
In freshwater fish, aluminum is a well-recognized gill toxicant, although responses are influenced by pH. Aluminum nanomaterials being used in diverse applications that likely to lead environmental release and exposure. However, it unclear if the effects of nanoparticulate similar those other forms or require special consideration. To examine acute toxicological exposure nanoparticle (Al-NP)s, adult female zebrafish were exposed either Al-NPs chloride for up 48 hours moderately hard fresh...
Background Queen conch (Strombus gigas) reproduction is inhibited in nearshore areas of the Florida Keys, relative to offshore environment where conchs reproduce successfully. Nearshore reproductive failure possibly a result exposure environmental factors, including heavy metals, which are likely accumulate close shore. Metals such as Cu and Zn detrimental many mollusks. Methodology/Principal Findings Histology shows gonadal atrophy compared reproductively healthy conchs. In order determine...
Fundamental questions remain about the application of omics in environmental risk assessments, such as consistency data across laboratories. The objective present study was to determine congruence transcript 6 independent Male fathead minnows were exposed a measured concentration 15.8 ng/L 17α-ethinylestradiol (EE2) for 96 h. Livers divided equally and sent participating laboratories transcriptomic analysis using same minnow microarray. Each laboratory free apply bioinformatics pipelines its...
Abstract Endocrine-disrupting chemicals are exogenous substances that can impact the reproduction of fish, potentially by altering circulating concentrations 17β-estradiol (E2), testosterone (T), and 11-ketotestosterone (11-KT). Common methods to measure steroids in plasma samples include radioimmunoassays (RIAs) enzyme-linked immunosorbant assays (ELISAs). The present study examines variability E2, T, 11-KT across 8 laboratories measuring reference pulp mill effluent-exposed white sucker...
Gammaherpesviruses, including the human pathogens Epstein⁻Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) are oncogenic viruses that establish lifelong infections in hosts associated with development of lymphoproliferative diseases lymphomas. Recent studies have shown majority mammalian genome is transcribed gives rise to numerous long non-coding RNAs (lncRNAs). Likewise, large double-stranded DNA genomes herpesviruses undergo pervasive transcription, expression many as...
The human gammaherpesviruses Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus are etiologic agents of numerous B cell lymphomas. A hallmark gammaherpesvirus infection is their ability to establish lifelong latency in cells. However, the specific mechanisms that mediate chronic cells
Fipronil is a phenylpyrazole pesticide that used in both residential and agricultural applications. detected run-off water systems are near areas which the has been applied. The acts to antagonize gamma aminobutyric acid receptors, leading over-excitation central nervous system. relatively high toxicity fish, but mechanisms underlying not well understood embryonic stages. Zebrafish embryos were exposed single concentration of fipronil for 48 h at ∼3-4 h-post-fertilization. Following 7-day...