A5040357793- Angiogenesis and VEGF in Cancer
- Cell Adhesion Molecules Research
- Platelet Disorders and Treatments
- Cancer-related molecular mechanisms research
- Axon Guidance and Neuronal Signaling
- Bioinformatics and Genomic Networks
- Gene expression and cancer classification
- Molecular Biology Techniques and Applications
- Glycosylation and Glycoproteins Research
- Chronic Lymphocytic Leukemia Research
- Hippo pathway signaling and YAP/TAZ
- Adipose Tissue and Metabolism
- Renal Diseases and Glomerulopathies
- HER2/EGFR in Cancer Research
- Protease and Inhibitor Mechanisms
- Congenital heart defects research
- Cancer, Hypoxia, and Metabolism
- Aquaculture disease management and microbiota
- Kruppel-like factors research
- Environmental Toxicology and Ecotoxicology
- Metabolomics and Mass Spectrometry Studies
- Complement system in diseases
- Protein Degradation and Inhibitors
- Single-cell and spatial transcriptomics
- Reproductive biology and impacts on aquatic species
University of Liverpool
2014-2022
University of Birmingham
2006-2016
NIHR Birmingham Biomedical Research Centre
2010-2013
Whiston Hospital
2012
Cancer Research UK
2008-2011
The Honourable Society of Lincoln's Inn
2008
John Radcliffe Hospital
2008
University of Oxford
2008
University of Auckland
1987
The platelet surface is poorly characterized due to the low abundance of many membrane proteins and lack specialist tools for their investigation. In this study we identified novel human mouse megakaryocyte using proteomics genomics approaches. Three separate methods were used enrich prior identification by liquid chromatography tandem mass spectrometry: lectin affinity chromatography, biotin/NeutrAvidin free flow electrophoresis. Many known, abundant transmembrane several each receptor...
This study aimed to further elucidate the function of Roundabout proteins in endothelium. We show that both Robo1 and Robo4 are present human umbilical vein endothelial cells (HUVECs) have knocked expression down using small interfering RNA (siRNA) technology. knockout were then studied a variety vitro assays. also performed yeast 2-hybrid analysis intracellular domain as bait identify interacting downstream signaling. Both siRNA knockdown transfection Robo4-green fluorescent protein...
The acquisition and analysis of datasets including multi-level omics physiology from non-model species, sampled field populations, is a formidable challenge, which so far has prevented the application systems biology approaches. If successful, these could contribute enormously to improving our understanding how populations living organisms adapt environmental stressors relating to, for example, pollution climate. Here we describe first network inference approach integrating transcriptional,...
Platelets have recently been shown to drive liver injury in murine models of viral hepatitis and promote regeneration through the release serotonin. Despite their emerging role inflammatory disease, little is known about mechanisms by which platelets bind hepatic vasculature. Therefore, we referenced public expression data determine profile potential adhesive receptors expressed endothelium. We then used a combination tissue-binding flow-based endothelial-binding adhesion assays show that...
Background Formation of blood vessels requires the concerted regulation an unknown number genes in a spatial-, time- and dosage-dependent manner. Determining genes, which drive vascular maturation is crucial for identification new therapeutic targets against pathological angiogenesis. Methology/Principal Findings We accessed global gene throughout chick chorio-allantoic membrane (CAM), highly vascularized tissue, using pan genomic microarrays. Seven percent analyzed showed significant change...
// Prisca Exertier 1,2 , Sophie Javerzat Baigang Wang 3,8 Mélanie Franco John Herbert 4 Natalia Platonova Marie Winandy 5 Nadège Pujol Olivier Nivelles 6 Sandra Ormenese 7 Virginie Godard Jürgen Becker 8 Roy Bicknell Raphael Pineau 9 Jörg Wilting 8* Andreas Bikfalvi 1,2* Martin Hagedorn 1 Univ. Bordeaux, LAMC, UMR 1029, F-33405 Talence, France 2 INSERM, 3 Ruhr-Universität Bochum, Medizinische Fakultät; Abt. f. Anatomie und Embryologie, D-44780 Germany...
NURF is a conserved higher eukaryotic ISWI-containing chromatin remodeling complex that catalyzes ATP-dependent nucleosome sliding. By sliding nucleosomes, able to alter dynamics control transcription and genome organization. Previous biochemical genetic analysis of the specificity-subunit Drosophila (Nurf301/Enhancer Bithorax (E(bx)) has defined as critical regulator homeotic, heat-shock steroid-responsive gene transcription. It been speculated controls pathway specific by co-operating with...
Platelets are essential for wound healing and inflammatory processes, but can also play a deleterious role by causing heart attack stroke. Normal platelet activation is dependent on tetraspanins, superfamily of glycoproteins that function as ‘organisers’ cell membranes recruiting other receptors signalling proteins into tetraspanin-enriched microdomains. However, our understanding how tetraspanin microdomains regulate platelets hindered the fact only four 33 mammalian tetraspanins have been...
Abstract Background In this study, differential gene expression analysis using complementary DNA (cDNA) libraries has been improved. Firstly by the introduction of an accurate method assigning Expressed Sequence Tags (ESTs) to genes and secondly, a novel likelihood ratio statistical scoring between two pools cDNA libraries. These methods were applied latest available cell line bulk tissue in two-step screen predict tumour endothelial markers. Initially, lines silico subtracted from...
Angiogenesis is essential for solid tumour growth, whilst the molecular profiles of blood vessels have been reported to be different between cancer types. Although presently available anti-angiogenic strategies are providing some promise treatment cancers it perhaps not surprisingly that, none agents work on all tumours. Thus, discovery novel targets, relevant individual types, required. Using Affymetrix microarray analysis laser-captured, CD31-positive we identified 63 genes that...
We aimed to characterize the expression and function of a novel transcript that bioinformatics analysis predicted be endothelial specific, called endothelial-specific molecule-2 (ECSM2).A full-length cDNA was isolated ECSM2 putative 205-amino acid transmembrane protein bears no homology any known protein. Quantitative polymerase chain reaction in vitro situ hybridization vivo confirmed exclusively endothelial, localization plasma membrane shown. Knockdown human umbilical vein cells using...
Lung cancer remains the leading cause of cancer-related death, largely owing to lack effective treatments. A tumour vascular targeting strategy presents an attractive alternative; however, molecular signature vasculature in lung is poorly explored. This work aimed identify novel targets cancer. Enzymatic digestion fresh tissue followed by endothelial capture with Ulex lectin-coated magnetic beads was used isolate endothelium from specimens patients. Endothelial isolates healthy and were...
In order to map the extracellular or membrane proteome associated with vasculature and stroma in an embryonic organism vivo, we developed a biotinylation technique for chicken embryo combined it mass spectrometry bioinformatic analysis. We also applied this procedure implanted tumors growing on chorioallantoic after induction of granulation tissue. Membrane matrix proteins were most abundant components identified. Relative quantitative analysis revealed differential protein expression...
A relatively large percentage of patients with chronic obstructive pulmonary disease (COPD) develop systemic co-morbidities that affect prognosis, among which muscle wasting is particularly debilitating. Despite significant research effort, the pathophysiology this important extrapulmonary manifestation still unclear. key question remains unanswered to what extent inflammatory mediators might play a role in pathology. Cigarette smoke (CS) main risk factor for developing COPD and therefore...
Fundamental questions remain about the application of omics in environmental risk assessments, such as consistency data across laboratories. The objective present study was to determine congruence transcript 6 independent Male fathead minnows were exposed a measured concentration 15.8 ng/L 17α-ethinylestradiol (EE2) for 96 h. Livers divided equally and sent participating laboratories transcriptomic analysis using same minnow microarray. Each laboratory free apply bioinformatics pipelines its...
Gliomas are a highly heterogeneous group of brain tumours that refractory to treatment, invasive and pro-angiogenic. Glioblastoma patients have an average survival time less than 15 months. Understanding the molecular basis different grades glioma, from well differentiated, low-grade high-grade tumours, is key step in defining new therapeutic targets. Here we use data-driven approach learn structure gene regulatory networks observational data resulting models formulate hypothesis on...
Abstract Background Modern functional genomic approaches may help to better understand the molecular events involved in tissue morphogenesis and identify signatures pathways. We have recently applied transcriptomic profiling evidence development of normal chicken chorioallantoic membrane (CAM) tumor engrafted on CAM. now extended our studies by performing a transcriptome analysis "wound model" CAM, which is another relevant model morphogenesis. Results To induce granulation (GT) formation,...
In recent years, decreases in fish populations have been attributed, part, to the effect of environmental chemicals on ovarian development. To understand underlying molecular events we developed a dynamic model ovary development linking gene transcription key physiological end points, such as gonadosomatic index (GSI), plasma levels estradiol (E2) and vitellogenin (VTG), largemouth bass (Micropterus salmoides). We were able identify specific clusters genes, which are affected at different...