A5054796837- Circadian rhythm and melatonin
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Tryptophan and brain disorders
- SARS-CoV-2 and COVID-19 Research
- Hepatitis C virus research
- HIV Research and Treatment
- Angiogenesis and VEGF in Cancer
- Dietary Effects on Health
- Cancer, Hypoxia, and Metabolism
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- COVID-19 Clinical Research Studies
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Analytical Chemistry and Chromatography
- Immune Cell Function and Interaction
- Respiratory viral infections research
- CAR-T cell therapy research
- Congenital Diaphragmatic Hernia Studies
- Genetics, Aging, and Longevity in Model Organisms
- Mosquito-borne diseases and control
- Virus-based gene therapy research
- Epigenetics and DNA Methylation
- Gut microbiota and health
University College London
2023-2025
Hainan Medical College Hospital
2025
University of Oxford
2017-2024
The First Affiliated Hospital, Sun Yat-sen University
2022
Sun Yat-sen University
2022
Chinese Academy of Medical Sciences & Peking Union Medical College
2021
University of Birmingham
2011-2020
Zhejiang University
2018
University of Southampton
2018
Abstract NP 105–113 -B*07:02-specific CD8 + T cell responses are considered among the most dominant in SARS-CoV-2-infected individuals. We found strong association of this response with mild disease. Analysis clones and single-cell sequencing were performed concurrently, functional avidity antiviral efficacy assessed using an vitro SARS-CoV-2 infection system, correlated receptor usage, transcriptome signature disease severity (acute n = 77, convalescent 52). demonstrated a beneficial cells...
Highlights•Hypoxia downregulates SARS-CoV-2 receptors ACE2 and TMPRSS2 inhibits viral entry•Hypoxic signaling replication particle genesis via HIF-1α•HIF prolyl hydroxylase inhibitors are a potential therapeutic option for COVID-19SummaryCOVID-19, caused by the novel coronavirus SARS-CoV-2, is global health issue with more than 2 million fatalities to date. Viral shaped cellular microenvironment, one important factor consider oxygen tension, in which hypoxia inducible (HIF) regulates...
Abstract The circadian clock regulates immune responses to microbes and affects pathogen replication, but the underlying molecular mechanisms are not well understood. Here we demonstrate that components BMAL1 REV-ERBα influence several steps in hepatitis C virus (HCV) life cycle, including particle entry into hepatocytes RNA genome replication. Genetic knock out of Bmal1 over-expression or activation REV-ERB with synthetic agonists inhibits replication HCV related flaviruses dengue Zika via...
Despite an unprecedented global research effort on SARS-CoV-2, early replication events remain poorly understood. Given the clinical importance of emergent viral variants with increased transmission, there is urgent need to understand stages and transcription. We used single-molecule fluorescence in situ hybridisation (smFISH) quantify positive sense RNA genomes 95% detection efficiency, while simultaneously visualising negative genomes, subgenomic RNAs, proteins. Our absolute quantification...
Engineering T cells to express chimeric antigen receptors (CARs) specific for antigens on hematological cancers has yielded remarkable clinical responses, but with solid tumors, benefit been more limited. This may reflect lack of suitable target antigens, immune evasion mechanisms in malignant cells, and/or cell infiltration into tumors. An alternative approach, circumvent these problems, is targeting the tumor vasculature rather than directly. CLEC14A a glycoprotein selectively...
The coronavirus disease 2019 pandemic, caused by severe acute respiratory syndrome 2 (SARS-CoV-2) coronavirus, is a global health issue with unprecedented challenges for public health. SARS-CoV-2 primarily infects cells of the tract via spike glycoprotein binding to angiotensin-converting enzyme (ACE2). Circadian rhythms coordinate an organism's response its environment and can regulate host susceptibility virus infection. We demonstrate that silencing circadian regulator Bmal1 or treating...
BackgroundTraditional classification systems of metabolic-associated fatty liver disease (MAFLD) do not account for the high rate extrahepatic complications. To create a new MAFLD using metabolic parameters to identify risks complications more accurately.MethodsThe retrospective study included patients from First Affiliated Hospital Sun Yat-sen University model development, and was validated respectively Chinese cohort UK Biobank database. Cluster analysis with k-means cluster built age,...
Abstract Chronic hepatitis B virus (HBV) infection is a major cause of liver disease and cancer worldwide for which there are no curative therapies. The challenge in curing eradicating or silencing the covalent closed circular DNA (cccDNA) form viral genome. circadian factors BMAL1/CLOCK REV-ERB master regulators transcriptome yet their role HBV replication unknown. We establish cycling cell-model demonstrate that directly regulates NTCP-dependent delta particle entry. Importantly, we show...
•Primate hepadnaviridae encode conserved hypoxia response elements.•Hypoxia inducible factors bind HBV DNA and activate the basal core promoter.•Pharmacological stabilization of low oxygen increases transcription particle genesis.•Knockdown studies show a role for both HIF-1α HIF-2α in regulating transcription. Background & AimsHypoxia (HIFs) are hallmark inflammation key regulators hepatic immunity metabolism, yet their replication is poorly defined. replicates hepatocytes within liver,...
Pathogen-specific CD8 + T cell responses restricted by the nonpolymorphic nonclassical class Ib molecule human leukocyte antigen E (HLA-E) are rarely reported in viral infections. The natural HLA-E ligand is a signal peptide derived from classical Ia HLA molecules that interact with NKG2/CD94 receptors to regulate killer functions, but pathogen-derived peptides can also be presented HLA-E. Here, we describe five severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) elicited...
Abstract Human immunodeficiency virus 1 (HIV-1) is a life-threatening pathogen that still lacks curative therapy or vaccine. Despite the reduction in AIDS-related deaths achieved by current antiretroviral therapies, drawbacks including drug resistance and failure to eradicate infection highlight need identify new pathways target infection. Circadian rhythms are endogenous 24-h oscillations which regulate physiological processes immune responses infection, there an emerging role for circadian...
The gut microbiota in the hepatitis B virus related acute-on-chronic liver failure (HBV-ACLF) is poorly defined. We aim to uncover characteristics of HBV-ACLF and other HBV associated pathologies. analyzed microbiome patients with or pathologies healthy individuals by 16S rRNA sequencing metagenomic fecal samples. 212 HBV-ACLF, 252 chronic (CHB), 162 HBV-associated cirrhosis (HBV-LC) 877 were recruited for study. CHB HBV-LC are grouped as HBV-Other. discovered striking differences diversity...
Chronic liver disease and cancer are global health challenges. The role of the circadian clock as a regulator physiology is well established in rodents, however, identity epigenetic regulation rhythmically expressed genes human less studied. Here we unravel rhythmic transcriptome epigenome hepatocytes using male chimeric mice. We identify large number protein coding mice, which includes key transcription factors, chromatin modifiers, critical enzymes. show that hepatitis C virus (HCV)...
Abstract Viral replication is defined by the cellular microenvironment and one key factor local oxygen tension, where hypoxia inducible factors (HIFs) regulate response to oxygen. Human immunodeficiency virus (HIV) infected cells within secondary lymphoid tissues exist in a low-oxygen or hypoxic environment vivo. However, majority of studies on HIV latency are performed under laboratory conditions HIFs inactive. We show role for HIF-2α restricting transcription via direct binding viral...
Understanding the host pathways that define susceptibility to Severe-acute-respiratory-syndrome-coronavirus-2 (SARS-CoV-2) infection and disease are essential for design of new therapies. Oxygen levels in microenvironment transcriptional landscape, however influence hypoxia on virus replication animal models is not well understood. In this study, we identify a role hypoxic inducible factor (HIF) signalling axis inhibit SARS-CoV-2 infection, epithelial damage respiratory symptoms Syrian...
The extract of medicinal plants is increasingly popular around the whole world due to its attractive therapeutic effects. However, bioavailability bioactive compounds was barely satisfactory easily deactivated and untargeted properties. use nanotechnology develop novel carrier delivery techniques for extracts has been proven have significant potential provides an amazing improvement in effect. Calcium carbonate nanoparticles (CaCO3 NPs), as representative biodegradable materials, are well...
Abstract Infusing virus-specific T cells is effective treatment for rare Epstein-Barr virus (EBV)–associated posttransplant lymphomas, and more limited success has been reported using this approach to treat a far common EBV-associated malignancy, nasopharyngeal carcinoma (NPC). However, current approaches EBV-transformed lymphoblastoid cell lines reactivate EBV-specific infusion take 2 3 months of in vitro culture favor outgrowth targeting viral antigens expressed within EBV+ but not NPC....
Background and Aims Therapeutic strategies against HBV focus, among others, on the activation of immune system to enable infected host eliminate HBV. Hypoxia‐inducible factor 1 alpha (HIF1α) stabilization has been associated with impaired responses. pathogenesis triggers chronic hepatitis‐related scaring, leading inter alia modulation liver oxygenation transient activation, both factors playing a role in HIF1α stabilization. Approach Results We addressed whether interferes immune‐mediated...
Supercritical anti-solvent fluidized bed (SAS-FB) coating technology has the advantages of reducing particle size, preventing high surface energy aggregation, improving dissolution performance and bioavailability insoluble drugs. The poor solubility Biopharmaceutics Classification System (BCS) class IV drugs poses challenges in achieving optimal bioavailability. Numerous anti-cancer including paclitaxel (PTX) belong to BCS IV, hindering their therapeutic efficacy. To address this concern,...