A5015267500- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Viral-associated cancers and disorders
- Bacteriophages and microbial interactions
- Dermatology and Skin Diseases
- Multiple Sclerosis Research Studies
- Immune Cell Function and Interaction
- RNA and protein synthesis mechanisms
- Parvovirus B19 Infection Studies
- Animal Virus Infections Studies
- Extracellular vesicles in disease
- Viral Infections and Vectors
- Exercise and Physiological Responses
- Bacterial Genetics and Biotechnology
- Hepatitis Viruses Studies and Epidemiology
- Protein Structure and Dynamics
- Asphalt Pavement Performance Evaluation
- Infrastructure Maintenance and Monitoring
- HIV Research and Treatment
- Polymer Nanocomposites and Properties
- Cancer-related molecular mechanisms research
- Vector-borne infectious diseases
University of Colorado Denver
2015-2024
University of Colorado Anschutz Medical Campus
2019-2024
Color (United States)
2019
University of Washington
2010-2011
Instituto Tecnológico de Ciudad Madero
2006
Many viruses express noncoding RNAs (ncRNAs). The gammaherpesviruses (γHVs), including Epstein-Barr virus, Kaposi's sarcoma-associated herpesvirus, and murine γHV68, each contain multiple ncRNA genes, microRNAs (miRNAs). While these ncRNAs can regulate host viral processes in vitro, the genetic contribution of to infection pathogenesis remains largely unknown. To study functional γHV infection, we have used a small-animal model pathogenesis. γHV68 encodes eight small hybrid that both...
The gammaherpesviruses, including Epstein-Barr virus (EBV), Kaposi's sarcoma-associated herpesvirus (KSHV), and murine gammaherpesvirus 68 (MHV68, MuHV-4, γHV68), are etiologic agents of a wide range lymphomas non-hematological malignancies. These viruses possess large highly dense dsDNA genomes that feature >80 bidirectionally positioned open reading frames (ORFs). abundance overlapping transcripts extensive splicing throughout these have until now prohibited high throughput-based...
This paper shows the state of art Mexican asphalt, AC-20 grade, modified by linear styrene−butadiene triblock copolymers (SBS), which include variations in their chemical structures, specifically, total styrene and 1,2-butadiene vinyl contents. The main objective this work was studying influence variation content between 9 to 41% (wt/wt), also 10 45% (wt/wt). Both variables were changed structure for SBS copolymer order see effect on thermal rheological properties when they are used as...
Varicella zoster virus (VZV) reactivates from ganglionic sensory neurons to produce herpes (shingles) in a unilateral dermatomal distribution, typically the thoracic region. Reactivation not only heightens risk of stroke and other neurological complications but also increases susceptibility co-infections with various viral bacterial pathogens at sites distant original infection. The mechanism by which VZV results remote initial foci remains unclear. Small extracellular vesicles (sEVs) are...
Investigating the dynamics of virus-host interactions in vivo remains an important challenge, often limited by ability to directly identify virally infected cells. Here, we utilize a beta-lactamase activated fluorescent substrate primary targets murine gammaherpesvirus 68 (MHV68) infection peritoneal cavity. By optimizing and detection conditions, were able achieve multiparameter characterization cells ensuing host response. MHV68 leads pronounced increase immune cells, with CD8+ T...
Gammaherpesviruses are common viruses associated with lifelong infection and increased disease risk. Reactivation from latency aids the virus in maintaining throughout life of host is responsible for a wide array outcomes. Previously, we demonstrated that virus-encoded cyclin (v-cyclin) murine gammaherpesvirus 68 (γHV68) essential optimal reactivation normal mice but not lacking tumor suppressor p18INK4c (p18). Whether p18 plays cell-intrinsic or -extrinsic role constraining remains unclear....
Abstract The gammaherpesviruses (γHVs) establish a lifelong infection in their hosts, with the cellular outcome of intimately regulated by target cell type. Murine gammaherpesvirus 68 (MHV68), small animal model γHV infection, infects macrophages vivo, resulting range outcomes, from lytic replication to latent infection. Here, we have further investigated nature MHV68 macrophage using reductionist and primary vivo studies. While readily infected J774 line, viral gene expression were...
Virus infection of adrenal glands can disrupt secretion mineralocorticoids, glucocorticoids, and sex hormones from the cortex catecholamines medulla, leading to a constellation symptoms such as fatigue, dizziness, weight loss, nausea, muscle joint pain. Specifically, varicella zoster virus (VZV) produce bilateral hemorrhage insufficiency during primary or following reactivation. However, mechanisms by which VZV affects are not well-characterized. Herein, we determined if human cortical cells...
Gammaherpesviruses are a prime example of how viruses can alter the host transcriptional landscape to establish infection. Despite major insights into these modify RNA polymerase II-dependent generation messenger RNAs, influence activity III remains much less clear.
Gammaherpesviruses establish life-long infections within their host and have been shown to be the causative agents of devastating malignancies. Chronic infection is mediated through cycles transcriptionally quiescent stages latency with periods reactivation into detectable lytic productive infection. The mechanisms that regulate from remain poorly understood. Previously, we defined a critical role for viral cyclin in promoting latency. Disruption had no impact on frequency cells containing...
Abstract Simian varicella virus (SVV) produces peripheral inflammatory responses during (primary infection) and zoster (reactivation) in rhesus macaques (RM). However, it is unclear if measures are accurate proxies for central nervous system (CNS) responses. Thus, we analyzed cytokine Aβ42/Aβ40 changes paired serum cerebrospinal fluid (CSF) the course of infection. During zoster, every RM had variable CSF levels compared to pre-inoculation levels. Overall, infection appears affect CNS...
Abstract Gammaherpesviruses establish life-long infections within their host and have been shown to be the causative agents of devastating malignancies. Chronic infection is mediated through cycles transcriptionally quiescent stages latency with periods reactivation into more active lytic productive infection. The mechanisms that regulate from remain poorly understood. Previously, we defined a critical role for viral cyclin in promoting latency. Disruption had no impact on frequency cells...
ABSTRACT RNA polymerase III (pol III) transcribes multiple non-coding (nc) RNAs that are essential for cellular function. Pol III-dependent transcription is also engaged during certain viral infections, including the gammaherpesviruses (γHVs), where pol ncRNAs promote pathogenesis. Additionally, several host upregulated γHV infection and play integral roles in pathogenesis by facilitating establishment gene expression. Here, we sought to investigate how promoters transcripts regulated...
Abstract Investigating the dynamics of virus-host interactions in vivo remains an important challenge, often limited by ability to directly identify virally-infected cells. Here, we combine detection a beta-lactamase activated fluorescent substrate with full spectrum flow cytometry primary targets murine gammaherpesvirus 68 (MHV68) infection peritoneal cavity. By optimizing and conditions, were able achieve multiparameter characterization infected cells ensuing host response. MHV68 leads...
ABSTRACT Gammaherpesvirus (γHV) non-coding RNAs (ncRNAs) are integral modulators of viral infection. The γHVs engage RNA polymerase III (pol III)-dependent transcription both host and ncRNAs, which contribute to establishment, gene expression, pathogenesis. Viral such as the EBV-encoded (EBERs), reportedly interact with multiple RNA-binding proteins (RBPs) inflammatory responses implicated in development malignancies. Here, we examined RBP interactions pol III-transcribed tRNA-miRNA encoded...