Gammaherpesviruses, including Epstein-Barr virus (EBV), Kaposi sarcoma-associated herpesvirus (KSHV, or HHV-8), and murine gammaherpesvirus 68 (MHV68, γHV68, MuHV-4), are B cell-tropic pathogens that each encode at least 12 microRNAs (miRNAs). It is predicted these regulatory RNAs facilitate infection by suppressing host target genes involved in a wide range of key cellular pathways. However, the precise contribution miRNAs make to viral life cycle pathogenesis vivo unknown. MHV68 mice...

Recent intense investigations have uncovered important functions for a diverse array of novel noncoding RNA (ncRNA) species, including microRNAs (miRNAs) and long RNAs. Not surprisingly, viruses from multiple families evolved to encode their own regulatory RNAs; however, the specific in vivo these ncRNAs are largely unknown. The human gammaherpesviruses Epstein-Barr virus (EBV) Kaposi's sarcoma-associated herpesvirus (KSHV) highly ubiquitous pathogens that associated with development wide...

The gammaherpesviruses, including Epstein-Barr virus (EBV), Kaposi's sarcoma-associated herpesvirus (KSHV), and murine gammaherpesvirus 68 (MHV68, MuHV-4, γHV68), are etiologic agents of a wide range lymphomas non-hematological malignancies. These viruses possess large highly dense dsDNA genomes that feature >80 bidirectionally positioned open reading frames (ORFs). abundance overlapping transcripts extensive splicing throughout these have until now prohibited high throughput-based...

The Epstein Barr virus (EBV) contributes to the tumor phenotype through a limited set of primarily non-coding viral RNAs, including 31 mature miRNAs. Here we investigated impact EBV miRNAs on remodeling cell transcriptome. Strikingly, displayed exceptionally abundant expression in primary EBV-associated Burkitt’s Lymphomas (BLs) and Gastric Carcinomas (GCs). To investigate miRNA targeting, used high-resolution approach, CLASH GC BL models. Affinity constant calculations targeting efficacies...

Gammaherpesviruses, including the human pathogens Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV), establish lifelong latent infection in B cells are associated with a variety of tumors. In addition to protein coding genes, these viruses encode numerous microRNAs (miRNAs) within their genomes. While putative host targets EBV KSHV miRNAs have been previously identified, specific functions during vivo largely unknown. Murine gammaherpesvirus 68 (MHV68) is natural...

Gammaherpesviruses, including the human pathogens Epstein⁻Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) are oncogenic viruses that establish lifelong infections in hosts associated with development of lymphoproliferative diseases lymphomas. Recent studies have shown majority mammalian genome is transcribed gives rise to numerous long non-coding RNAs (lncRNAs). Likewise, large double-stranded DNA genomes herpesviruses undergo pervasive transcription, expression many as...

The oncogenic gammaherpesviruses, including human Epstein-Barr virus (EBV), Kaposi's sarcoma-associated herpesvirus (KSHV), and murine gammaherpesvirus 68 (MHV68, γHV68, MuHV-4), are associated with numerous malignancies, B cell lymphomas nasopharyngeal carcinoma. These viruses employ molecular strategies to colonize the host, expression of noncoding RNAs (ncRNAs). As first viral ncRNAs identified, EBV-encoded RNA 1 2 (EBER1 EBER2, respectively) have been investigated extensively for...

Gammaherpesviruses encode proteins with homology to the cellular purine metabolic enzyme formyl-glycinamide-phosphoribosyl-amidotransferase (FGARAT), but role of these viral FGARATs (vFGARATs) in pathogenesis a natural host has not been investigated. We report novel for ORF75A vFGARAT murine gammaherpesvirus 68 (MHV68) infectious virion production and colonization mice. MHV68 mutants premature stop codons orf75A exhibited log reduction acute replication lungs after intranasal infection,...

SUMMARY Accurate quantification of gene and transcript‐specific expression, with the underlying knowledge precise transcript isoforms, is crucial to understanding many biological processes. Analysis RNA sequencing data has benefited from development alignment‐free algorithms which enhance precision speed expression analysis. However, such require a reference transcriptome. Here we generate dataset (LsRTDv1) for lettuce (cv. Saladin), combining long‐ short‐read publicly available...

Gammaherpesviruses are widespread pathogens causing persistent infections linked to the development of numerous types lymphomas in humans. During latency, most viral protein-coding genes suppressed, facilitating evasion adaptive immune recognition protein antigens. In contrast, many noncoding RNA (ncRNA) molecules expressed infected cells and can regulate key cellular pathways while simultaneously evading recognition. To counteract this, express internal pattern receptors that intrinsically...

ABSTRACT Epstein Barr virus (EBV) contributes to the tumor phenotype through a limited set of primarily non-coding viral RNAs, including 31 mature miRNAs. Here we investigated impact EBV miRNAs on remodeling cell transcriptome. Strikingly, displayed exceptionally abundant expression in primary EBV-associated Burkitt’s Lymphomas (BLs) and Gastric Carcinomas (GCs). To investigate miRNA targeting, used high-resolution approach, CLASH GC BL models. Affinity constant calculations targeting...

Abstract The transcription of mammalian genomes has been shown to possess an intriguing complexity and numerous novel RNA molecules have identified in the last 10-15 years. Viruses with large DNA genomes, especially herpesviruses, are also generate many different species some them may function as long non-coding RNAs. Viral harbor several genes within close proximity each other can multigenic transcripts addition commonly observed antisense transcription. It is essential study biological...

Gammaherpesviruses are associated with multiple types of tumor development and understanding the pathogenesis these viruses has been subject various studies. Throughout lytic latent life cycle, utilize numerous virally encoded microRNAs (miRNAs) to regulate key mechanisms infected cell in their favor. Therefore, it is important understand miRNA mRNA target interactions for developing better therapeutics. In this study, strategy design a recombinant virus expressing short hairpin RNA (shRNA)...

The transcription of mammalian genomes exhibits an intriguing complexity and numerous novel RNA molecules have been identified. Viruses with large DNA genomes, especially herpesviruses, generate many different species, including long non-coding RNAs (lncRNAs). Dense viral can multigenic transcripts in addition to commonly observed antisense transcripts. It is essential study the biological roles these aside from protein-coding counterparts. Multiple open reading frame (ORF) 63-64 locus...

Yeni geliştirilen RNA dizileme teknolojileri ile yaklaşık son on yıldır, memeli genomlarının önceden çöp, ‘junk’ DNA olarak görülen kısımlarının aslında aktif RNA’ya dönüştükleri gözlemlenmektedir. Yapılan biyoinformatik analizler ve proteomik çalışmalar, bu ürünlerinin çok büyük bir kısmının proteine dönüşmediğini göstermektedir. Uzun kodlamayan adlandırılan sınıftaki genlerin, günümüzde, bilinen genlerden sayıca daha fazla olduğu ortaya çıkarılmıştır. Bu moleküllerinin nasıl üretildikleri...

Abstract Gammaherpesviruses are associated with multiple types of tumour development and understanding the pathogenesis these viruses has been subject many different studies. Throughout lytic latent life cycle, utilize numerous virally encoded miRNAs to regulate key mechanisms infected cell in their favour. Therefore it is important understand miRNA mRNA target interactions for developing better therapeutics. In this study, strategy design a short hairpin encoding RNA element targeting...