A5002551784- Viral-associated cancers and disorders
- Lymphoma Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Cytomegalovirus and herpesvirus research
- Prostate Cancer Treatment and Research
- Chronic Lymphocytic Leukemia Research
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Estrogen and related hormone effects
- RNA Research and Splicing
- Bioinformatics and Genomic Networks
- Circular RNAs in diseases
- Eosinophilic Disorders and Syndromes
- Machine Learning in Bioinformatics
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cancer-related Molecular Pathways
- Computational Drug Discovery Methods
- Ubiquitin and proteasome pathways
- Immune Cell Function and Interaction
- Sarcoma Diagnosis and Treatment
- Hormonal and reproductive studies
- Medical Imaging and Pathology Studies
- Histiocytic Disorders and Treatments
- Receptor Mechanisms and Signaling
Tulane University
2015-2024
Cancer Genetics (United States)
2021
Louisiana Cancer Research Center
2007-2015
Louisiana State University
1985-2013
MUSC Hollings Cancer Center
2013
Medical University of South Carolina
2013
Louisiana State University Health Sciences Center New Orleans
2009-2013
Shanghai East Hospital
2013
Beth Israel Deaconess Medical Center
2003
Harvard University
1988-2003
ABSTRACT The Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP1) is a functional homologue of the tumor necrosis factor receptor family and contributes substantially to oncogenic potential EBV through activation nuclear κB (NF-κB). MicroRNAs (miRNAs) are class small RNA molecules that involved in regulation cellular processes such as growth, development, apoptosis have recently been linked cancer phenotypes. Through miRNA microarray analysis, we demonstrate LMP1 dysregulates...
Abstract Constitutively active androgen receptor splice variants (AR-V) lacking the ligand-binding domain have been implicated in pathogenesis of castration-resistant prostate cancer and mediating resistance to newer drugs that target axis. AR-V regulates expression both canonical AR targets a unique set cancer-specific are enriched for cell-cycle functions. However, little is known about how controls gene expression. Here, we report two major AR-Vs, termed AR-V7 ARv567es, not only...
The cellular microRNA miR-155 has been shown to be involved in lymphocyte activation and is expressed Epstein-Barr virus (EBV)-infected cells displaying type III latency gene expression but not I expression. We show here that the elevated levels of due EBV epigenetic differences cell lines tested, we EBV-infected requires a conserved AP-1 element promoter. Gene analysis was carried out line transduced with an miR-155-expressing retrovirus. This identified both miR-155-suppressed -induced...
Oxidative stress is believed to be an important mediator of neurodegeneration. However, the transcriptional pathways induced in neurons by oxidative that activate protective gene responses have yet fully delineated. We report transcription factor Sp1 acetylated response neurons. Histone deacetylase (HDAC) inhibitors augment acetylation, DNA binding, and Sp1-dependent expression confer resistance stress-induced death vitro vivo . activation necessary for effects HDAC inhibitors. Together,...
Expression of the Epstein-Barr virus (EBV) BZLF1 gene in latently infected lymphocytes is sufficient to trigger viral lytic cycle. As shown accompanying report (E. Flemington and S.H. Speck, J. Virol. 64:1217-1226, 1990), promoter for (Zp) contains two distinct types elements (ZI ZII [an AP-1-like domain]) which are responsive phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), an inducer Although Zp can be activated with TPA EBV-negative Burkitt's lymphoma cell line (Ramos), its...
microRNA-155 is an oncogenic microRNA that has been shown to be critical for B-cell maturation and immunoglobulin production in response antigen. In line with its function activation, miR-155, primary transcript, integration cluster (BIC), induced by receptor (BCR) cross-linking. Using pharmacological inhibitors the human line, Ramos, we show activation of BIC miR-155 expression BCR signaling occurs through extracellular signaling-regulated kinase (ERK) c-Jun N-terminal (JNK) pathways but...
Regulation of gene expression in mammals through methylation cytosine residues at CpG dinucleotides is involved the development and progression tumors. Because many genes that are control cell proliferation regulated by members E2F family transcription factors because some DNA-binding sites methylated vivo , we have investigated whether can regulate functions. We show here elements derived from dihydrofolate reductase, E2F1, cdc2 promoters prevents binding all tested (E2F1 E2F5). In...
The E2F family of transcription factors plays a key role in regulating cell-cycle progression. Accordingly, is itself tightly controlled by series transcriptional and posttranscriptional events. Here we provide evidence that E2F1 protein levels are regulated the ubiquitin–proteasome-dependent degradation pathway. An analysis mutants identified conserved carboxyl-terminal region, which required for eliciting ubiquitination turnover. Fusion this sequence to heterologous protein, GAL4, resulted...
The high level of accuracy and sensitivity next generation sequencing for quantifying genetic material across organismal boundaries gives it tremendous potential pathogen discovery diagnosis in human disease. Despite this promise, substantial bacterial contamination is routinely found existing human-derived RNA-seq datasets that likely arises from environmental sources. This raises the need stringent analysis protocols studies investigating sequence-based microbial signatures clinical samples.
We observed that microRNAs (miRNAs) regulate differentiation in a variety of simpler systems also human multipotent stromal cells (hMSCs) from bone marrow. Differentiation hMSCs into osteoblasts and adipocytes was inhibited by using lentiviruses expressing shRNAs to decrease expression Dicer Drosha, two enzymes process early transcripts miRNA. Expression analysis miRNAs during hMSC identified 19 were up-regulated osteogenic 20 adipogenic differentiation, 11 which commonly both...
The product of the Epstein-Barr virus BZLF1 gene encodes a protein which is related to c-fos, it has been shown bind specifically consensus AP-1 site, and its expression in latently virus-infected lymphocytes sufficient trigger viral lytic cycle. We identified several elements within promoter (Zp) are responsive phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), an inducer These fall into two classes based on factors these sequences their resulting functional behavior. Four homologous...
Epstein-Barr virus (EBV) is associated with roughly 10% of gastric carcinomas worldwide (EBVaGC). Although previous investigations provide a strong link between EBV and carcinomas, these studies were performed using selected gene probes. Using cohort carcinoma RNA-seq data sets from The Cancer Genome Atlas (TCGA), we quantitative global assessment expression in assessed cellular pathway alterations. transcripts detected 17% samples but varied significantly coverage depth. In four the highest...
Our appreciation for the extent of Epstein Barr virus (EBV) transcriptome complexity continues to grow through findings EBV encoded microRNAs, new long non-coding RNAs as well more recent discovery over a hundred polyadenylated lytic transcripts. Here we report an additional layer identification repertoire latent and viral circular RNAs. Utilizing RNase R-sequencing with cell models representing latency types I, II, III, identified expressed from Cp promoter involving backsplicing W1 W2...
Using a simple viral genome enrichment approach, we report the de novo assembly of Akata and Mutu Epstein-Barr virus (EBV) genomes from single lane next-generation sequencing (NGS) reads. The are type I EBV strains approximately 171 kb in length. Evidence for heterogeneity was found but not strain. A comparative analysis with another four completely sequenced strains, B95-8/Raji, AG876, Mutu, GD1, demonstrated that strain is most closely related to GD1 exhibits greatest divergence II strain,...
Abstract Background The tumor microenvironment is a crucial determinant in progression. Interstitial extracellular matrix (ECM), such as type I collagen (Col-1), aberrantly enriched the and promotes Long intergenic non-coding RNAs (lincRNA) are new family of regulatory that modulate fundamental cellular processes via diverse mechanisms. Findings We investigated whether expression lincRNAs was regulated by promoting Col-1. In three-dimensional organotypic culture model using reconstituted...
L1 elements represent the only currently active, autonomous retrotransposon in human genome, and they make major contributions to genetic instability. The vast majority of 500 000 genome are defective, a relatively few can contribute retrotransposition process. However, there is no comprehensive approach identify specific loci that actively transcribed separate from excess L1-related sequences co-transcribed within genes. We have developed RNA-Seq procedures, as well 1200 bp 5΄ RACE product...
Annotation of herpesvirus genomes has traditionally been undertaken through the detection open reading frames and other genomic motifs, supplemented with sequencing individual cDNAs. Second generation high-density microarray studies have revealed vastly greater transcriptome complexity than is captured by existing annotation. The pervasive nature overlapping transcription throughout genomes, however, poses substantial problems in resolving transcript structures using these methods alone. We...
Sex is an important determinant of brain microvessels (MVs) function and susceptibility to cerebrovascular neurological diseases, but underlying mechanisms are unclear. Using high throughput RNA sequencing analysis, we examined differentially expressed (DE) genes in MVs from young, male, female rats. Bioinformatics analysis the 23,786 identified indicates that 298 (1.2%) were DE using False Discovery Rate criteria (FDR; p < 0.05), which 119 (40%) 179 (60%) abundantly male MVs,...
Thirteen and 10 sequences of the Alu family repeated DNA elements found within human thymidine kinase beta-tubulin genes, respectively, were compared. These genes have approximately five times expected density members. The consensus sequence that could be drawn from these 23 members would differ slightly others random but only at very heterogeneous positions. different do show pairwise percentage identities, with 15% (7 48 analyzed) them clearly representing a separate subfamily sequences....