A5027062675- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- DNA and Nucleic Acid Chemistry
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- HIV Research and Treatment
- Viral gastroenteritis research and epidemiology
- Advanced biosensing and bioanalysis techniques
- Enzyme Structure and Function
- DNA Repair Mechanisms
- Microbial infections and disease research
- Molecular Biology Techniques and Applications
- Insect Resistance and Genetics
- Gene Regulatory Network Analysis
- RNA modifications and cancer
- Glycosylation and Glycoproteins Research
- Chromosomal and Genetic Variations
- Polyomavirus and related diseases
- Cytomegalovirus and herpesvirus research
- Protein Structure and Dynamics
- Biochemical and Molecular Research
- Evolution and Genetic Dynamics
University of Iowa
2014-2024
University of California, San Diego
2023
University of Montana
2023
University of Colorado Denver
2023
University of Colorado Health
1996-2006
Iowa City Public Library
1999
University of Oregon
1983
Stanford University
1972-1974
University of Washington
1969
University of Illinois Urbana-Champaign
1965
ABSTRACT Plaque-forming, galactose-transducing lambda strains have been isolated from lysogens in which bacterial genes removed between the galactose operon and prophage by deletion mutation.—A second class has starting with a lysogenic strain carries of to right part prophage. This was lysogenized phage yield lysogen galactose-transducing, plaque-forming phages were obtained. These found be genetically unstable, due duplication chromosome. The genetic instability these partial diploid is...
Terminase enzymes are common to complex double-stranded DNA viruses and function package viral into the capsid. We recently demonstrated that bacteriophage lambda terminase gpA gpNu1 proteins assemble a stable heterotrimer with molar ratio gpA1/gpNu1(2). This protomer possesses maturation packaging activities dependent on E. coli integration host factor protein (IHF). Here, we show further assembles homogeneous tetramer of protomers composition (gpA1/gpNu1(2))4. Electron microscopy shows...
INTRODUCTION The head of bacteriophage λ is assembled when the products two separate synthetic pathways come together. One, DNA replication pathway, produces a linear polymer chromosomes called concatemer. In other proteins assemble to produce empty capsid shell known as prohead (Georgopoulos et al., this volume). and proheads interact, chromosome from concatemer inserted into cavity separated unencapsidated remainder by endonucleolytic cutting. Knowledge assembly was presented Yarmolinsky...
A key step in the assembly of many viruses is packaging DNA into preformed procapsids by an ATP-powered molecular motor. To shed light on motor mechanism we used single-molecule optical tweezers measurements to study effect mutations large terminase subunit bacteriophage lambda dynamics. mutation, K84A, putative ATPase domain driving translocation was found decrease velocity approximately 40% but did not change force dependence or processivity substantially. These findings support hypothesis...
Terminase, the DNA packaging enzyme of bacteriophage λ, is a heteromultimer gpNu1 (21 kDa) and gpA (74 subunits, encoded by λ Nu1 A genes, respectively. Sequence comparisons indicate that both have match to P-loop motif ATPase centers, which glycine-rich segment followed lysine. By site-specific mutagenesis, we changed lysines putative P-loops (K35) (K497) arginine, alanine, or aspartic acid, studied mutant enzymes kinetic analysis photochemical cross-linking with 8-azido-ATP. Both subunits...