A5058346480- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Bacterial Genetics and Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- DNA and Nucleic Acid Chemistry
- HIV Research and Treatment
- Enzyme Structure and Function
- Protein Structure and Dynamics
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Hemoglobin structure and function
- Viral gastroenteritis research and epidemiology
- Protein purification and stability
- Polyomavirus and related diseases
- Herpesvirus Infections and Treatments
- Heme Oxygenase-1 and Carbon Monoxide
- SARS-CoV-2 and COVID-19 Research
- Transgenic Plants and Applications
- Porphyrin and Phthalocyanine Chemistry
- Electron Spin Resonance Studies
- Skin Protection and Aging
- Cytomegalovirus and herpesvirus research
- Virology and Viral Diseases
University of Montana
2015-2025
University of Colorado Denver
1993-2025
University of Colorado Anschutz Medical Campus
2015-2024
University of Iowa
1996-2023
University of California, San Diego
2023
Massachusetts Institute of Technology
2023
University of Puerto Rico-Mayaguez
2021
University of Washington
2009-2019
University of Colorado Health
1996-2008
The University of Texas System
2005
Reaction of horse myoglobin with H2O2 oxidizes the iron to ferryl (Fe(IV) = O) state and produces a protein radical that is rapidly dissipated by poorly understood mechanisms. As reported here, reaction results in covalent binding up 18% prosthetic heme group protein. The chromophore protein-bound very similar itself. High performance liquid chromatography tryptic digests indicates formation heme-bound peptides associated disappearance peptide sequence YLE-FISDAIIHVLHSK corresponding...
Methemoglobin and metmyoglobin catalyze the H2O2-dependent oxidation of styrene to oxide benzaldehyde. The formation requires molecular oxygen as well H2O2 but does not, shown by inhibitor studies, involve superoxide or hydroxyl radicals. Approximately 38, 67, 78% in derives from 18O2 reactions catalyzed, respectively, bovine hemoglobin, sperm whale myoglobin, equine heart whereas 70, 55, 35% can be derived [18O]H2O2. However, a larger fraction epoxide than suggested labeling data (perhaps...
Chloroperoxidase and HzOz oxidize styrene to oxide phenylacetaldehyde but not benzaldehyde.The epoxide oxygen is shown by studies with Hz'sOz derive quantitatively from the peroxide.The epoxidation of tram-[ l-'H]styrene chloroperoxidase proceeds without detectable loss stereochemistry, as does rat liver cytochrome P-450, although much more produced than P-450.Chloroperoxidase P-450 thus closely related oxygentransfer mechanisms.Horseradish peroxidase 2,4,6-trimethylphenol...
Abstract Biomotor-driven DNA packaging is a key step in the life cycle of many viruses. We previously developed single-molecule methods using optical tweezers to measure dynamics bacteriophage lambda motor. The system more complex than others examined via assays with respect substrate and ancillary proteins required. Because this, previous studies which efficiently detected events used crude E. coli cell extracts containing host factors terminase enzyme. However, use suboptimal for...
Terminase enzymes are common to complex double-stranded DNA viruses and function package viral into the capsid. We recently demonstrated that bacteriophage lambda terminase gpA gpNu1 proteins assemble a stable heterotrimer with molar ratio gpA1/gpNu1(2). This protomer possesses maturation packaging activities dependent on E. coli integration host factor protein (IHF). Here, we show further assembles homogeneous tetramer of protomers composition (gpA1/gpNu1(2))4. Electron microscopy shows...
Herpes simplex type 1 virus (HSV-1) and bacteriophage λ capsids undergo considerable structural changes during self-assembly DNA packaging. The initial steps of viral capsid require weak, non-covalent interactions between the subunits to ensure free energy minimization error-free assembly. In final stages packaging, however, internal genome pressure dramatically increases, requiring significant strength withstand high pressures tens atmospheres. Our data reveal that loosely formed structure...
Terminases are enzymes common to complex double-stranded DNA viruses and required for packaging of the viral genome into a preformed capsid. The overexpression bacteriophage lambda-terminase in Escherichia coli has been previously reported (Chow, S., Daub, E., Murialdo, H. (1987) Gene (Amst.) 60, 277-289), we present here purification scheme isolation milligram quantities protein which is homogenous ( > 97%) as determined by SDS-polyacrylamide gel electrophoresis. lambda-Terminase composed...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInteraction of Escherichia coli DNA polymerase I with azidoDNA and fluorescent probes: identification protein-DNA contactsCarlos Enrique Catalano, Dwayne J. Allen, Stephen BenkovicCite this: Biochemistry 1990, 29, 15, 3612–3621Publication Date (Print):April 17, 1990Publication History Published online1 May 2002Published inissue 17 April 1990https://pubs.acs.org/doi/10.1021/bi00467a004https://doi.org/10.1021/bi00467a004research-articleACS...
| Several diseases exhibit a high degree of heterogeneity and diverse reprogramming cellular pathways. To address this complexity, additional strategies technologies must be developed to define their scope variability with the goal improving current treatments. Nanomedicines derived from viruses are modular systems that can easily adapted for combinatorial approaches, including imaging, biomarker targeting, intracellular delivery therapeutics. Here, we describe "designer nanoparticle" system...
Abstract The response by vaccine developers to the COVID-19 pandemic has been extraordinary with effective vaccines authorized for emergency use in United States within 1 year of appearance first cases. However, emergence SARS-CoV-2 variants and obstacles global rollout new highlight need platforms that are amenable rapid tuning stable formulation facilitate logistics delivery worldwide. We developed a “designer nanoparticle” platform using phage-like particles (PLPs) derived from...
Terminase, the DNA packaging enzyme of bacteriophage λ, is a heteromultimer gpNu1 (21 kDa) and gpA (74 subunits, encoded by λ Nu1 A genes, respectively. Sequence comparisons indicate that both have match to P-loop motif ATPase centers, which glycine-rich segment followed lysine. By site-specific mutagenesis, we changed lysines putative P-loops (K35) (K497) arginine, alanine, or aspartic acid, studied mutant enzymes kinetic analysis photochemical cross-linking with 8-azido-ATP. Both subunits...