A5044171138- Reproductive biology and impacts on aquatic species
- Effects and risks of endocrine disrupting chemicals
- Environmental Toxicology and Ecotoxicology
- Pharmaceutical and Antibiotic Environmental Impacts
- Toxic Organic Pollutants Impact
- Fish Ecology and Management Studies
- Aquaculture Nutrition and Growth
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Computational Drug Discovery Methods
- Pesticide and Herbicide Environmental Studies
- Animal testing and alternatives
- Animal Genetics and Reproduction
- Estrogen and related hormone effects
- Physiological and biochemical adaptations
- Metabolomics and Mass Spectrometry Studies
- Vehicle Routing Optimization Methods
- Per- and polyfluoroalkyl substances research
- Molecular Biology Techniques and Applications
- Aquaculture disease management and microbiota
- Hormonal and reproductive studies
- Health Systems, Economic Evaluations, Quality of Life
- Water Quality and Resources Studies
- Transportation and Mobility Innovations
- CRISPR and Genetic Engineering
- Advanced Manufacturing and Logistics Optimization
Environmental Protection Agency
2016-2025
NOAA Great Lakes Environmental Research Laboratory
2023
Mid-Continent University
2019
Research Triangle Park Foundation
2016
University of Ottawa
2015
University of Houston
2014
Mississippi State University
2014
University of Minnesota
2014
University of Saskatchewan
2014
VA Office of Research and Development
2013
An adverse outcome pathway (AOP) is a conceptual framework that organizes existing knowledge concerning biologically plausible, and empirically supported, links between molecular-level perturbation of biological system an at level organization regulatory relevance. Systematic information into AOP frameworks has potential to improve decision-making through greater integration more meaningful use mechanistic data. However, for the scientific community collectively develop useful knowledgebase...
Surface water from 38 streams nationwide was assessed using 14 target-organic methods (719 compounds). Designed-bioactive anthropogenic contaminants (biocides, pharmaceuticals) comprised 57% of 406 organics detected at least once. The 10 most-frequently anthropogenic-organics included eight pesticides (desulfinylfipronil, AMPA, chlorpyrifos, dieldrin, metolachlor, atrazine, CIAT, glyphosate) and two pharmaceuticals (caffeine, metformin) with detection frequencies ranging 66–84% all sites....
The U.S. Environmental Protection Agency (EPA) is faced with the challenge of efficiently and credibly evaluating chemical safety often limited or no available toxicity data. expanding number chemicals found in commerce environment, coupled time resource requirements for traditional testing exposure characterization, continue to underscore need new approaches. In 2005, EPA charted a course address this by embracing computational toxicology (CompTox) investing technologies capabilities push...
Organization of existing and emerging toxicological knowledge into adverse outcome pathway (AOP) descriptions can facilitate greater application mechanistic data, including those derived through high-throughput in vitro, high content omics imaging, biomarker approaches, risk-based decision making. The previously ad hoc process AOP development is being formalized internationally harmonized guidance principles. goal this article was to outline the information desired for formal description...
Systematic consideration of scientific support is a critical element in developing and, ultimately, using adverse outcome pathways (AOPs) for various regulatory applications. Though weight evidence (WoE) analysis has been proposed as basis assessment the maturity and level confidence an AOP, methodologies tools are still being formalized. The Organization Economic Co-operation Development (OECD) Users' Handbook Supplement to Guidance Document Developing Assessing AOPs (OECD 2014a; hereafter...
Based on the results of a Horizon Scanning exercise sponsored by Society Environmental Toxicology and Chemistry that focused advancing adverse outcome pathway (AOP) framework, development guidance related to AOP network was identified as critical need. This not only included questions focusing directly networks, but also topics such mixture toxicity assessment implementation feedback loops within framework. A set two articles has been developed begin exploring these concepts. In present...
A quantitative adverse outcome pathway (qAOP) consists of one or more biologically based, computational models describing key event relationships linking a molecular initiating (MIE) to an outcome. qAOP provides quantitative, dose-response, and time-course predictions that can support regulatory decision-making. Herein we describe several facets qAOPs, including (a) motivation for development, (b) technical considerations, (c) evaluation confidence, (d) potential applications. The used as...
Total concentrations of several known xenobiotic estrogen receptor (ER) agonists and natural synthetic were measured in water by use a combination instrumental bioanalytical approaches. Samples from 3 municipal wastewater treatment plants (WWTPs) south central Michigan (upstream effluent); 4 point source locations on the Trenton Channel Detroit River, MI; 5 Lake Mead, NV analyzed. Organic compounds extracted L samples using solid-phase extraction disks separated into three fractions based...
Abstract Relative potency (REP) estimates are widely used to characterize and compare the of a wide variety samples analyzed using in vitro bioassays. generally calculated as simple ratio: EC50 well-characterized standard divided by sample. Such valid only when dose-response curves for sample parallel exhibit same maximum achievable response (efficacy). These conditions often either violated or cannot be demonstrated. As result, there is need calculate present REPs manner that addresses...
Polychlorinated naphthalenes (PCNs) are ubiquitous environmental pollutants that structurally similar to other polychlorinated diaromatic hydrocarbons (PCDHs), such as dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), and biphenyls (PCBs). Despite being ubiquitous, much less is known about the fate, transport, biological effects of individual PCN congeners than PCDHs. The purpose current study was utilize an in vitro assay (H4IIE-luc) determine potencies relative...
Masan Bay, located on the southeast coast of Korea, is a long and narrow inlet semi-closed bay that receives industrial municipal wastewaters from cities Changwon. In this study, sediments collected 28 locations Bay were analyzed for total organic carbon (TOC), nonylphenol (NP), octylphenol (OP), bisphenol A (BPA), organochlorine pesticides (HCB, HCHs, CHLs, DDTs), individual polychlorinated biphenyl (PCB) congeners, 16 polycyclic aromatic hydrocabons (PAHs) to characterize their spatial...
Abstract The dioxinlike and estrogenic relative potencies (REPs) of 16 priority polycyclic aromatic hydrocarbons (PAHs), seven methylated PAHs, two hydroxylated PAHs were examined using three in vitro cell bioassays. An ethoxyresorufin‐ O ‐deethylase assay with PLHC‐1 fish hepatoma cells luciferase H4IIE‐luc recombinant rat used to evaluate potency. MVLN, human breast carcinoma cells, was Seven the tested induced significant responses. Excluding outliers large ranges uncertainty, REPs for...
Abstract A challenge in the field of ecotoxicology is linkage alterations at molecular and biochemical levels organization to adverse outcomes individuals populations. In present study, a predictive relationship between plasma vitellogenin (VTG) concentration fecundity female fathead minnows (Pimephales promelas) was derived from 21-d laboratory toxicity tests with five chemicals (17β-trenbolone, 17α-trenbolone, prochloraz, fenarimol, fadrozole) that inhibit VTG production through different...
The elementary shortest-path problem with resource constraints (ESPPRC) is a widely used modeling tool in formulating vehicle-routing and crew-scheduling applications. ESPPRC often occurs as subproblem of an enclosing problem, where it to generate implicitly the set all feasible routes or schedules, column-generation formulation time windows (VRPTW). As NP-hard strong sense, classical solution approaches are based on corresponding nonelementary (SPPRC), which can be solved using...
Many chemicals that adversely affect reproduction and/or development do so through multiple pathways within the reproductive tract and hypothalamic-pituitary-gonadal axis. Notable in this regard are fungicides, such as prochloraz or fenarimol, which mammals have potential to impact endocrine function inhibition of CYP enzymes involved steroid metabolism, well antagonism androgen receptor(s). The objective our studies was assess effects fenarimol on a model small fish species, fathead minnow...
In the 20th century, predicting ecological risk from use of certain chemicals relied on testing programs that directly measured adverse outcomes (death, disease, reproductive failure, or developmental dysfunction) using in vivo toxicity tests.Extrapolation these tests-from one species to another controlled laboratory tests uncontrolled real-world environments-was based largely conservative assumptions arbitrary uncertainty factors.The result?Costly, time-consuming, unfocused, and contentious...
Conservation of a molecular target across species can be used as line-of-evidence to predict the likelihood chemical susceptibility. The web-based Sequence Alignment Predict Across Species Susceptibility (SeqAPASS; https://seqapass.epa.gov/seqapass/) application was developed simplify, streamline, and quantitatively assess protein sequence/structural similarity taxonomic groups means relative intrinsic intent tool is allow for evaluation any potential while remaining amenable variable...
Toxicological responses to stressors are more complex than the simple one-biological-perturbation one-adverse-outcome model portrayed by individual adverse outcome pathways (AOPs). Consequently, AOP framework was designed facilitate de facto development of networks that can aid in understanding and prediction pleiotropic interactive effects common environmentally realistic, exposure scenarios. The present study introduces nascent concepts related qualitative analysis networks. First, graph...