A5036474832- Reproductive biology and impacts on aquatic species
- Carcinogens and Genotoxicity Assessment
- Aquaculture Nutrition and Growth
- Effects and risks of endocrine disrupting chemicals
- Fish Ecology and Management Studies
- Environmental Toxicology and Ecotoxicology
- Pharmaceutical and Antibiotic Environmental Impacts
- Toxic Organic Pollutants Impact
- Animal testing and alternatives
- Computational Drug Discovery Methods
- Animal Genetics and Reproduction
- Estrogen and related hormone effects
- Genetics, Bioinformatics, and Biomedical Research
- Water Quality and Pollution Assessment
- Reproductive Biology and Fertility
- Mercury impact and mitigation studies
- Marine Toxins and Detection Methods
- High-Velocity Impact and Material Behavior
- Smoking Behavior and Cessation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Pesticide Residue Analysis and Safety
- Gene Regulatory Network Analysis
- Mesoporous Materials and Catalysis
- Zeolite Catalysis and Synthesis
- Transportation Safety and Impact Analysis
Arizona State University
2017-2024
Environmental Protection Agency
2022
Obihiro University of Agriculture and Veterinary Medicine
2020-2021
Oregon Health & Science University
2004-2017
Faculdade Evangélica do Paraná
2014
University of Maryland, College Park
2009
University of Florida
2009
Walker (United States)
2004-2007
Tulane University
1997-2004
University Medical Center New Orleans
1999
A quantitative adverse outcome pathway (qAOP) consists of one or more biologically based, computational models describing key event relationships linking a molecular initiating (MIE) to an outcome. qAOP provides quantitative, dose-response, and time-course predictions that can support regulatory decision-making. Herein we describe several facets qAOPs, including (a) motivation for development, (b) technical considerations, (c) evaluation confidence, (d) potential applications. The used as...
Abstract Background Aquatic organisms are continuously exposed to complex mixtures of chemicals, many which can interfere with their endocrine system, resulting in impaired reproduction, development or survival, among others. In order analyze the effects and mechanisms action estrogen/anti-estrogen mixtures, we male fathead minnows ( Pimephales promelas ) for 48 hours via water 2, 5, 10, 50 ng 17α-ethinylestradiol (EE 2 )/L, 100 ZM 189,154/L (a potent antiestrogen known block activity...
A working group at the National Institute for Mathematical and Biological Synthesis (NIMBioS) explored feasibility of integrating 2 complementary approaches relevant to ecological risk assessment. Adverse outcome pathway (AOP) models provide "bottom-up" mechanisms predict specific toxicological effects that could affect an individual's ability grow, reproduce, and/or survive from a molecular initiating event. Dynamic energy budget (DEB) offer "top-down" approach reverse engineers stressor on...
An adverse outcome pathway (AOP) is a sequence of key events from molecular-level initiating event and an ensuing cascade steps to with population-level significance. To implement predictive strategy for ecotoxicology, the multiscale nature AOP requires computational models link salient processes (e.g., in chemical uptake, toxicokinetics, toxicodynamics, population dynamics). A case study domoic acid was used demonstrate strategies enable generic recommendations developing effort move toward...
We reanalyze the acute toxicity data on cancer chemotherapeutic agents compiled by Freireich et al. (1) and Schein al . (2) to derive coefficients of allometric equation for scaling toxic doses across species (toxic dose = a ‐[body weight] b ). In doing so, we extend analysis Travis White ( Risk Analysis , 1988, 8, 119‐125)by addressing uncertainties inherent in including hamster data, previously not used. Through Monte Carlo sampling, specifically account measurement errors when deriving...
Abstract Toxicant deliveries (by machine smoking) are compiled and associated cancer risks calculated for 13 carcinogens from 26 brands of conventional cigarettes categorized as “regular” (R), “light” (Lt), or “ultralight” (ULt), a reference cigarette. Eight “potentially reduced exposure product” (PREP) also considered. Because agency-to-agency differences exist in the slope factor (CSF) values adopted some carcinogens, two CSF sets were used calculations: set I [U.S. Environmental...
Endocrine disrupting chemicals (EDCs) are known to contaminate aquatic environments and alter the growth reproduction of organisms. The objective this study was evaluate sensitivity utility fathead minnow (Pimephales promelas) early life-stages as a model measure effects estrogenic antiestrogenic EDCs on physiological gene expression endpoints relative reproduction. Embryos (<24-h postfertilization, hpf) were exposed potent estrogen (17α-ethinyl estradiol, EE(2) , 2, 10, 50 ng L(-1)); weak...
Trenbolone, an anabolic androgen, and flutamide, antiandrogen, are prototypical model compounds for agonism antagonism of the androgen receptor. We hypothesized that 48 h exposures female fathead minnows (Pimephales promelas) to environmentally relevant concentrations these chemicals would alter genes regulated by receptor a mixture two block effects. Gene expression in ovaries was analyzed using minnow-specific 22 000-gene microarray. Flutamide altered about twice number as trenbolone, most...
Estrogenic chemicals in the aquatic environment have been shown to cause a variety of reproductive anomalies fish including full sex reversal, intersex, and altered population ratios. Two estrogens found environment, 17α-ethinylestradiol (EE2) 17β-estradiol (E2), measured wastewater treatment effluents adverse effects fish. To further our understanding how estrogen exposure affects endpoints male fathead minnow (FHM, Pimephales promelas), physiologically based computational model was...
Abstract Background Endocrine disrupting chemicals (e.g., estrogens, androgens and their mimics) are known to affect reproduction in fish. 17α-ethynylestradiol is a synthetic estrogen used birth control pills. 17β-trenbolone relatively stable metabolite of trenbolone acetate, androgen as growth promoter livestock. Both have been found the aquatic environment fish reproduction. In this study, we developed physiologically-based computational model for female fathead minnows (FHM, Pimephales...
Endocrine-disrupting chemicals can affect reproduction and development in humans wildlife. We developed a computational model of the hypothalamic-pituitary-gonadal (HPG) axis female fathead minnows to predict dose-response time-course (DRTC) behaviors for endocrine effects aromatase inhibitor, fadrozole (FAD). The describes adaptive responses stress involving regulated secretion generic gonadotropin (LH/FSH) from hypothalamic-pituitary complex. For development, we used plasma 17β-estradiol...
Abstract Recently, researchers have begun looking at changes in gene expression the fathead minnow ( Pimephales promelas ) after contaminant exposure as a way to develop biomarkers of and effects. However, bulk this research has been conducted on adults, with few studies focusing early life stages. Expression selected genes important growth, development, reproduction teleosts was quantified by quantitative polymerase chain reaction during different developmental time periods (from 0 28 d...
A computational model of oocyte growth dynamics (i.e., recruitment, growth, and spawning) in a batch-spawning fish, fathead minnow (FHM, Pimephales promelas), has been developed. The provides quantitative link between biochemical processes FHMs through the absorption vitellogenin (a lipoprotein precursor egg yolk proteins) into oocytes, which contributes significantly to fish. simulates number volume oocytes different batches within FHM ovary. Model-predicted clutch sizes spawning intervals...
Abstract Linking organismal‐level processes to underlying suborganismal mechanisms at the molecular, cellular and organ level constitutes a major challenge for predictive ecological risk assessments. This can be addressed with simple bioenergetic models in family of dynamic energy budget (DEB), which consist small number state equations quantifying universal processes, such as feeding, maintenance, development, reproduction growth. Motivated by need process‐based evaluate impact endocrine...
Bioaccumulation models are used to describe chemical uptake and clearances by organisms. Averaged input parameter values traditionally yield point estimates of model outputs. Hence, the uncertainty variability predictions ignored. Probabilistic modeling approaches, such as Monte Carlo simulation Bayesian method, have been recommended U.S. Environmental Protection Agency provide a quantitative description degree and/or in risk ecological hazards human health effects. In this study, analysis...
Fish spawning is often used as an integrated measure of reproductive toxicity, and indicator aquatic ecosystem health in the context forecasting potential population-level effects considered important for ecological risk assessment. Consequently, there a need flexible, widely-applicable, biologically-based models that can predict changes fecundity response to chemical exposures, based on readily measured biochemical endpoints, such plasma vitellogenin (VTG) concentrations, input parameters....