A5019301306- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Multiple Sclerosis Research Studies
- Immune Response and Inflammation
- Neuroinflammation and Neurodegeneration Mechanisms
- Diabetes and associated disorders
- Cytokine Signaling Pathways and Interactions
- Mast cells and histamine
- Autoimmune and Inflammatory Disorders Research
- Peripheral Neuropathies and Disorders
- Monoclonal and Polyclonal Antibodies Research
- Infectious Diseases and Tuberculosis
- IL-33, ST2, and ILC Pathways
- Systemic Lupus Erythematosus Research
- Blood groups and transfusion
- Single-cell and spatial transcriptomics
- HIV Research and Treatment
- Reproductive System and Pregnancy
- Gut microbiota and health
- Polyomavirus and related diseases
- Atherosclerosis and Cardiovascular Diseases
- Immunodeficiency and Autoimmune Disorders
- Glycosylation and Glycoproteins Research
- interferon and immune responses
Inserm
2010-2024
Université de Lille
2016-2024
Centre Hospitalier Universitaire de Lille
2022-2024
Lille Neurosciences & Cognition
2022-2024
Université Toulouse III - Paul Sabatier
2007-2017
Centre National de la Recherche Scientifique
2011-2017
Centre de Physiopathologie de Toulouse-Purpan
2006-2017
Lille Inflammation Research International Center
2016-2017
Université de Toulouse
2002-2016
Medical University of Vienna
2013
Abstract Although deficiencies in the NKT cell population have been observed multiple sclerosis and mouse strains susceptible to experimental autoimmune encephalomyelitis (EAE), little is known about function of these cells CNS autoimmunity. In this work we report that TCR Vα14-Jα281 transgenic nonobese diabetic mice, which are enriched CD1d-restricted cells, protected from EAE. The protection associated with a striking inhibition Ag-specific IFN-γ production spleen, implying modulation...
We studied the immunological basis for very potent encephalitogenicity of myelin/oligodendrocyte glycoprotein (MOG), a minor component myelin in CNS that is widely used to induce experimental autoimmune encephalomyelitis (EAE). For this purpose, we generated mutant mouse lacking functional mog gene. This MOG-deficient presents no clinical or histological abnormalities, permitting us directly assess role MOG as target autoantigen EAE. In contrast WT mice, which developed severe EAE following...
Abstract CD8 T cells are emerging as important players in multiple sclerosis (MS) pathogenesis, although their direct contribution to tissue damage is still debated. To assess whether autoreactive can contribute the pronounced loss of oligodendrocytes observed MS plaques, we generated mice which model Ag influenza hemagglutinin selectively expressed oligodendrocytes. Transfer preactivated hemagglutinin-specific led inflammatory lesions optic nerve, spinal cord, and brain. These lesions,...
The tyrosine kinase 2 variant rs34536443 has been established as a genetic risk factor for multiple sclerosis in variety of populations. However, the functional effect this on disease pathogenesis remains unclear. This study replicated association with cohort 1366 French patients and 1802 controls. Furthermore, we assessed consequences polymorphism human T lymphocytes by comparing reactivity cytokine profile isolated from individuals expressing protective TYK2GC genotype disease-associated...
The long serum t1/2 of IgGs is ensured by their interaction with the neonatal Fc receptor (FcRn), which salvages IgG from intracellular degradation. glycosylation thought not to influence FcRn binding and longevity in vivo. In this article, we demonstrate that hypersialylation asparagine 297 (N297) enhances persistence. This polarized achieved using a novel mutation, glutamate residue deletion at position 294 (Del) endows an up 9-fold increase lifespan. strongest impact was observed when Del...
Thymus-derived regulatory T cells (Tregs) expressing CD4, CD25, and the transcription factor Foxp3 play major roles in preventing autoimmunity. The Treg population is enriched high-avidity self-reactive cell receptors, thymic epithelial self-antigens (Ag) have been implicated their induction and/or selection. However, selection events leading to lineage commitment remain unclear. We followed development of self-Ag-specific Tregs double-transgenic mice coexpressing a neo-self-Ag,...
To determine the pathophysiologic features of progressive multifocal leukoencephalopathy (PML) associated with immune reconstitution inflammatory syndrome (PML-IRIS) in HIV-infected patients.In a cross-sectional study, we retrospectively analyzed 11 patients firm diagnosis PML-IRIS. Brain biopsies were collected from 5 and their histopathologic compared to those 4 classic PML.PML-IRIS developed soon after initiation antiretroviral therapy late-presenting patients. The lesions biopsied...
T cells differentiate into functionally distinct effector subsets in response to pathogen encounter. Cells of the innate immune system direct this process; CD1d-restricted invariant natural killer (iNKT) cells, for example, can either promote or inhibit Th(1) and Th(2) responses. Recently, a new subset CD4(+) helper called Th(17), was identified that is implicated mucosal immunity autoimmune disorders. To investigate influence iNKT on differentiation naïve we used an adoptive transfer model...
Abstract Invariant NKT cells are CD1d-restricted T specific for glycolipid Ags. Their activation or transgenic enrichment abrogates the development of experimental autoimmune encephalomyelitis (EAE). Herein, we demonstrate that in NKT-enriched mice protection from EAE is associated with infiltration CNS and local expression CD1d. This indicates acquires potential presentation when exposed to inflammatory stress, permitting triggering cells. To address importance CD1d-mediated Ag...
Abstract An increase in IL-17–producing CD8+ T (Tc17) cells has been reported the peripheral blood of children with recent onset type 1 diabetes (T1D), but their contribution to disease pathogenesis is still unknown. To directly study pathogenic potential β cell-specific Tc17 cells, we used an experimental model T1D based on expression neo-self Ag hemagglutinin (HA) pancreas. When transferred alone, HA-specific homed pancreatic lymph nodes without causing any infiltration or tissue...
Abstract Pertussis toxin (PTx) is a bacterial used to enhance the severity of experimental autoimmune diseases such as encephalomyelitis. It known promote permeabilization blood-brain barrier, maturation APC, activation autoreactive lymphocytes and alteration lymphocyte migration. In this study, we show that i.v. injection PTx in mice induces decrease number splenic CD4+CD25+ regulatory T cells (Treg cells). Furthermore, not only depletion dominant CD4+CD25+Foxp3+ subpopulation Treg cells,...
Abstract Multiple sclerosis (MS) is a demyelinating inflammatory disease of the CNS. Though originally believed to be CD4-mediated, additional immune effector mechanisms, including myelin-specific CD8+ T cells, are now proposed participate in pathophysiology MS. To study immunologic and encephalitogenic behavior HLA-A*0201-binding myelin-derived epitopes vivo, we used humanized HLA-A*0201-transgenic mouse model. Eight peptides derived from myelin oligodendrocyte glycoprotein (MOG), an...
Abstract Invariant NKT (iNKT) cells have been implicated in the regulation of autoimmune diseases. In several models type 1 diabetes, increasing number iNKT prevents development disease. Because CD8 T play a crucial role pathogenesis we investigated influence on diabetogenic cells. present study, diabetes was induced by transfer specific for influenza virus hemagglutinin into recipient mice expressing Ag specifically their β pancreatic contrast to previous reports, high frequency promoted...
The key role of B cells in the pathophysiology multiple sclerosis (MS) is supported by presence oligoclonal bands cerebrospinal fluid, association meningeal ectopic cell follicles with demyelination, axonal loss and reduction astrocytes, as well high efficacy lymphocyte depletion controlling inflammatory parameters MS. Here, we use a spontaneous model experimental autoimmune encephalomyelitis (EAE) to study clonality response targeting myelin oligodendrocyte glycoprotein (MOG). In...
Polyspecific T cells recognizing multiple distinct self-antigens have been identified in sclerosis and other organ-specific autoimmune diseases, but their pathophysiological relevance remains undetermined. Using a mouse model of sclerosis, we show that encephalomyelitis induction is strictly dependent on reactivation pathogenic by peptide (35-55) derived from myelin oligodendrocyte glycoprotein (MOG). This disease-inducing response wanes after onset. Strikingly, the progression disease...