A5000860494- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Tuberculosis Research and Epidemiology
- Immune Response and Inflammation
- Mycobacterium research and diagnosis
- Immune cells in cancer
- Glycosylation and Glycoproteins Research
- Reproductive System and Pregnancy
- vaccines and immunoinformatics approaches
- Eosinophilic Esophagitis
- IL-33, ST2, and ILC Pathways
- Cytomegalovirus and herpesvirus research
- Diabetes and associated disorders
- Immunodeficiency and Autoimmune Disorders
- Insect Resistance and Genetics
- Pancreatitis Pathology and Treatment
- Infectious Diseases and Tuberculosis
- HIV Research and Treatment
- Complement system in diseases
- Gut microbiota and health
- Escherichia coli research studies
- Viral Infections and Outbreaks Research
- Clostridium difficile and Clostridium perfringens research
Université de Toulouse
2011-2025
Centre National de la Recherche Scientifique
2010-2025
Université Toulouse III - Paul Sabatier
2011-2025
Institut de Pharmacologie et de Biologie Structurale
2013-2025
Centre National pour la Recherche Scientifique et Technique (CNRST)
2010
Inserm
2001-2007
Centre de Gestion Scientifique
2007
Centre de Physiopathologie de Toulouse-Purpan
2002-2004
Institut Universitaire de France
2004
Centre Hospitalier Universitaire de Toulouse
2002-2003
Abstract Upon encounter of a CTL with target cell carrying foreign Ags, the TCR internalizes its ligand, peptide-MHC class I complex. However, it is unclear how this can happen mechanistically because MHC molecules are anchored to cell’s surface via transmembrane domain. By using antigenic peptides and lipids that were fluorescently labeled, we found CTLs promptly capture membranes together peptide as well various other proteins. This efficient specific process requires sustained signaling....
Tuberculosis (TB), caused by the airborne bacterial pathogen Mycobacterium tuberculosis, remains a major source of morbidity and mortality worldwide. So far, study host-pathogen interactions in TB has mostly focused on physiology virulence pathogen, as well various innate adaptive immune compartments host. Microbial organisms endogenous to our body, so-called microbiota, interact not only with invading pathogens, but also system. Yet, impact microbiota host defense against M. tuberculosis...
Abstract T cell tolerance to self Ags is in part established the thymus by induction of apoptosis or anergy potentially autoreactive thymocytes. Some autospecific cells nevertheless migrate peripheral lymphoid organs but are kept under control recently identified CD4+CD25+ regulatory subset. Because these inhibit autoimmunity more efficiently than useful non-self Ag-specific immune responses, they probably autospecific, posing important questions as how develop thymus. In this study we show...
Abstract Occasional EBV infection of human NK cells may lead to malignant diseases such as naso-pharyngeal lymphoma although do not express CD21, the primary receptor for EBV. Here we show that during early in patients, attacked EBV-infected autologous B cells. In vitro, activated by conjugation CD21+ B-EBV cell targets transiently acquired a weak phenotype synaptic transfer few molecules onto their own membrane. presence viral particles, these ectopic receptors allowed binding novel host....
Rationale: In addition to their well-known function as antibody-producing cells, B lymphocytes can markedly influence the course of infectious or noninfectious diseases via antibody-independent mechanisms. tuberculosis (TB), cells accumulate in lungs, yet functional contribution host response remains poorly understood.Objectives: To document role TB an unbiased manner.Methods: We generated transcriptome isolated from Mycobacterium (Mtb)-infected mice and validated identified key pathways...
Significance Tuberculosis (TB) is an immunopathology, mostly of the lung, due to overexuberant immune response bacterial pathogen Mycobacterium tuberculosis . Here, we demonstrate in vitro and vivo that dendritic cell (DC) immunoreceptor (DCIR), a C-type lectin receptor expressed by DCs, modulates immunity TB sustaining type I IFN signaling DCs. These findings were generalized beyond TB, model antigen-presentation assay unrelated M. , suggesting they may extend other pathologies, such as...
Tissue-resident innate lymphoid cells (ILCs) regulate tissue homeostasis, protect against pathogens at mucosal surfaces, and are key players the interface of adaptive immunity. How ILCs adapt their phenotype function to environmental cues within tissues remains be fully understood. Here, we show that Mycobacterium tuberculosis (Mtb) infection alters lung IL-18Rα+ ILC toward a protective interferon-γ-producing ILC1-like population. This differentiation is controlled by type 1 cytokines...
Infection with lymphocytic choriomeningitis virus induces the generation of CD8+ cytotoxic T lymphocytes (CTL). In H-2b mouse, this cellular immune response is directed against three viral structural epitopes (GP1, GP2, and NP) presented by major histocompatibility complex (MHC) class I H-2Db molecules. This study was undertaken to delineate which sequence each these optimal for MHC binding CTL recognition. The first step synthesize relevant peptides truncated at N or C terminus flanking...
Prior to delivery of a lethal hit, NK cells form an immunological synapse scan the target and engage their activatory inhibitory receptors. Using freshly isolated cells, IL-2-activated polyclonal bulk or NKL cell line, we report here that early during this recognition process, human actively capture membrane fragments. This novel function occurs via synapse, is controlled by Src kinase, ATP, Ca2+ PKC involves rearrangements actin cytoskeleton. Furthermore, process down-regulated bysignals...
Abstract Key events of T and B cell biology are regulated through direct interaction with APC or target cells. Trogocytosis is a process whereby CD4+ T, CD8+ cells capture their specific membrane-bound Ag the acquisition plasma membrane fragments from cellular targets. With aim investigating whether ability to trigger trogocytosis was selective property receptors, we set up an assay that allowed us test many different surface molecules trogocytosis. On basis analysis series on cells,...
Objective: Compared with cell-free viral infection, virological synapses increase HIV capture by target cells, infectivity and cytopathicity, are believed to be less sensitive antibody neutralization. We have evaluated the impact of antibodies against envelope glycoproteins (gp120 gp41) on cell-to-cell transmission. Methods: analyzed role trogocytosis in transmission inhibitory mechanisms antigp120 IgGb12 antigp41 4E10 2F5 using cocultures NL4-3 or BaL-infected MOLT/CCR5 cells primary CD4 T...
Abstract The lungs harbor multiple resident microbial communities, otherwise known as the microbiota. There is an emerging interest in deciphering whether pulmonary microbiota modulate local immunity, and this knowledge could shed light on mechanisms operating response to respiratory pathogens. In study, we investigate capacity of a Lactobacillus strain lung T cell compartment assess its prophylactic potential upon infection with Mycobacterium tuberculosis, etiological agent tuberculosis....
Abstract Using H-2Kd-restricted CTL clones, which are specific for a photoreactive derivative of the Plasmodium berghei circumsporozoite peptide PbCS252–260 (SYIPSAEKI) and permit assessment TCR-ligand interactions by TCR photoaffinity labeling, we have previously identified several variants binding efficiency Ag recognition deviated fivefold or more. Here report that functional response (cytotoxicity IFN-γ production) correlated with rate complex dissociation, but not avidity binding. While...
Abstract Exchange of plasma membrane fragments, including cell-surface proteins and lipids, in conjugates formed between lymphocytes their cellular partners is a field intense investigation. Apart from its natural occurrence during Ag recognition, the process transfer can be triggered experimental or therapeutic settings when targeted by Abs are conjugated to FcγR-expressing accessory cells. The direction capture (i.e., which two cells going donate accept fragments) have important functional...
The granuloma is an elaborated aggregate of immune cells found in non-infectious as well infectious diseases. It a hallmark tuberculosis (TB). Predominantly thought host-driven strategy to constrain the bacilli and prevent dissemination, recent discoveries indicate can also be modulated into efficient tool promote microbial pathogenesis. aim future studies will certainly focus on better characterization mechanisms driving modulation functions. Here, we provide unique perspectives from both...
ABSTRACT The polarization of the monocyte/macrophage compartment toward an anti‐inflammatory profile is considered detrimental in tuberculosis (TB), but factors controlling M2 this context are still poorly understood. Here, we found that B cells promote differentiation human monocytes M2‐like activation program through a process primarily dependent on IL‐6 and STAT3 signaling monocytes. This confers with immunomodulatory properties characterized by reduced ability to produce proinflammatory...
Abstract B, αβ T, and NK lymphocytes establish immunological synapses (IS) with their targets to enable recognition. Transfer of target cell-derived Ags together proximal molecules onto the effector cell appears also occur through synapses. Little is known about molecular basis this transfer, but it assumed result from Ag receptor internalization. Because human γδ T cells recognize soluble nonpeptidic phosphoantigens as well tumor such Daudi, unknown whether they IS with, extract from,...
Recognition by CD8+ cytotoxic T lymphocytes (CTLs) of antigenic peptides bound to major histocompatibility class (MHC) I molecules on target cells leads sustained calcium mobilization and CTL degranulation resulting in perforin-dependent killing. We report that β1 β3 integrin-mediated adhesion extracellular matrix proteins and/or surfaces dramatically promotes degranulation. CTLs, when adhered fibronectin but not suspension, efficiently degranulate upon exposure soluble MHC·peptide...
We have investigated the density of peptides required to elicit different biological responses in cytotoxic T lymphocytes (CTL), including trogocytosis (i.e., phenomenon whereby actively capture fragments plasma membrane from those cells with which they establish an immune synapse). used two separate mouse models CTL recognising defined presented by MHC class I molecules. In both systems, triggering cytotoxicity and components reached saturation low densities ligand. On other hand,...