A5069550552- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Cytokine Signaling Pathways and Interactions
- Psychiatry, Mental Health, Neuroscience
- interferon and immune responses
- T-cell and B-cell Immunology
- Artificial Immune Systems Applications
- NF-κB Signaling Pathways
- Parasites and Host Interactions
- Protein Tyrosine Phosphatases
- Helicobacter pylori-related gastroenterology studies
- Glycosylation and Glycoproteins Research
- Cognitive Science and Education Research
- Galectins and Cancer Biology
- IL-33, ST2, and ILC Pathways
- Asthma and respiratory diseases
- Antifungal resistance and susceptibility
- Cell Image Analysis Techniques
- Mosquito-borne diseases and control
- Bacterial Infections and Vaccines
- Allergic Rhinitis and Sensitization
Urologische Klinik München
2024
Universitätsklinikum Erlangen
2015-2024
Friedrich-Alexander-Universität Erlangen-Nürnberg
2015-2024
Institute of Medical Microbiology and Hygiene
2006-2023
University of Salzburg
2001-2017
Johns Hopkins University Applied Physics Laboratory
2012-2013
Technical University of Munich
1999-2012
Paracelsus Medical University
2011-2012
Bombardier (Canada)
2011
TU Wien
2006-2010
IL-10 regulates inflammation by reducing cytokine and chemokine production from activated macrophages. We performed microarray experiments to identify possible effector molecules of investigate the global effect on transcriptional response induced in LPS-activated To exclude background effects endogenous IL-10, macrophages IL-10-deficient mice were used. up-regulated expression a small number genes (26 37 after 45 min 3 h, respectively), including newly identified previously documented...
Abstract The mycobacterial cord factor trehalose-6,6-dimycolate (TDM) and its synthetic analog trehalose-6,6-dibehenate (TDB) are potent adjuvants for Th1/Th17 vaccination that activate Syk-Card9 signaling in APCs. In this study, we have further investigated the molecular mechanism of innate immune activation by TDM TDB. Syk-coupling adapter protein FcRγ was essential macrophage Th17 adjuvanticity. FcRγ-associated C-type lectin receptor Mincle expressed macrophages upregulated Recombinant...
Novel vaccination strategies against Mycobacterium tuberculosis (MTB) are urgently needed. The use of recombinant MTB antigens as subunit vaccines is a promising approach, but requires adjuvants that activate antigen-presenting cells (APCs) for elicitation protective immunity. mycobacterial cord factor Trehalose-6,6-dimycolate (TDM) and its synthetic analogue Trehalose-6,6-dibehenate (TDB) effective in combination with vaccine candidates mice. However, it unknown which signaling pathways...
Abstract TLR are primary triggers of the innate immune system by recognizing various microorganisms through conserved pathogen-associated molecular patterns. TLR2 is receptor for a functional recognition bacterial lipopeptides (LP) and up-regulated during disorders such as chronic obstructive pulmonary disease sepsis. This unique in its ability to form heteromers with TLR1 or TLR6 mediate intracellular signaling. According fatty acid pattern well assembling polypeptide tail, LP can signal...
It is now emerging that for vaccines against a range of diseases including influenza, malaria and HIV, the induction humoral response insufficient substantial complementary cell-mediated immune necessary adequate protection. Furthermore, some such as tuberculosis, cellular seems to be sole effector mechanism required The development new adjuvants capable inducing highly complex responses with strong antigen-specific T-cell in addition antibodies therefore urgently needed.Herein, we describe...
Activation of the mitogen-activated protein kinase (MAPK) cascade after Toll-like receptor stimulation enables innate immune cells to rapidly activate cytokine gene expression. A balanced response signals infectious danger requires that cellular activation is transient. Here, we identify MAPK phosphatase dual specificity 1 (DUSP1) as an essential endogenous regulator inflammatory lipopolysaccharide (LPS). DUSP1-deficient (DUSP1−/−) bone marrow–derived macrophages showed selectively prolonged...
Abstract The cytokines IL-4 and IL-13 inhibit the production of NO from activated macrophages through an unresolved molecular mechanism. We show here that regulate depletion arginine, substrate inducible synthase (iNOS). Inhibition murine stimulated with LPS IFN-γ by or was dependent on Stat6, cell density in cultures, pretreatment for at least 6 h. IL-4/IL-13 did not interfere expression activity iNOS but up-regulated arginase I (the liver isoform arginase) a Stat6-dependent manner....
Abstract Arginase I expression in the liver must remain constant throughout life to eliminate excess nitrogen via urea cycle. In contrast, arginase macrophages is silent until signals from Th2 cytokines such as IL-4 and IL-13 are received mRNA then induced four five orders of magnitude. hypothesized play a regulatory potentially pathogenic role diseases asthma, parasitic, bacterial, worm infections by modulating NO levels promoting fibrosis. We show that Th2-inducible mouse controlled an...
Abstract Bronchial epithelial cells represent the first line of defense against invading airborne pathogens. They are important contributors to innate mucosal immunity and provide a variety antimicrobial effectors. However, surfaces prone contact with pathogenic, as well nonpathogenic microbes, therefore, immune recognition principles have be tightly controlled avoid uncontrolled permanent activation. TLRs been shown recognize conserved microbial patterns mediate inducible activation...
Abstract Emerging evidence suggests an important role for human epidermal keratinocytes in innate immune mechanisms against bacterial and viral skin infections. The proinflammatory effect of infections can be mimicked by double-stranded RNA (dsRNA). Herein, we demonstrate that express all known dsRNA sensing receptors at a constitutive inducible level, they use several downstream signaling pathways leading to broad pattern gene expression, not only response genes under the control NF-κB, but...
Suppressor of cytokine signaling (SOCS) proteins are inhibitors cytoplasmic Janus kinases (Jak) and signal transducer activator transcription (STAT) pathways. Previously the authors surprisingly observed that SOCS1 translocated into nucleus, which was because presence a nuclear localization sequence. This report now hypothesizes mediates specific functions within compartment it is instantly transported as shown by photoactivation live cell imaging in human HEK293 cells. The NFkB component...
ABSTRACT Classical twin studies and recent linkage analyses of African populations have revealed a potential involvement host genetic factors in susceptibility or resistance to Mycobacterium tuberculosis infection. In order identify the candidate genes involved test their causal implication, we capitalized on mouse model tuberculosis, since inbred strains also differ substantially Two susceptible two resistant were aerogenically infected with 1,000 CFU M. , regulation gene expression was...
NF-κB is a key transcriptional regulator of inflammatory responses, but also controls expression prosurvival genes, whose products protect tissues from damage and may thus act indirectly in an antiinflammatory fashion. The variable importance these two distinct NF-κB-controlled responses impacts the potential utility inhibition as treatment strategy for intractable conditions, such bowel disease. Here, we show murine models that IKKβ-dependent activation exacerbates acute inflammation,...
Elevated IL-10 has been implicated in reactivation tuberculosis (TB). Since macrophages rather than T cells were reported to be the major source of TB, we analyzed consequences a macrophage-specific overexpression transgenic mice (macIL-10-transgenic) after aerosol infection with Mycobacterium (Mtb). MacIL-10 more susceptible chronic Mtb nontransgenic littermates, exhibiting higher bacterial loads lung 12 wk and dying significantly earlier controls. The differentiation, recruitment,...
Successful vaccination against intracellular pathogens requires the generation of cellular immune responses. Trehalose-6,6-dibehenate (TDB), synthetic analog mycobacterial cord factor trehalose-6,6-dimycolate (TDM), is a potent adjuvant inducing strong Th1 and Th17 We previously identified C-type lectin Mincle as receptor for these glycolipids that triggers FcRγ-Syk-Card9 pathway APC activation adjuvanticity. Interestingly, in vivo data revealed effect was not solely Mincle-dependent but...