Biofilms are complex bacterial communities that resist the action of antibiotics and human immune system. Bacteria within biofilms cause numerous, almost impossible to eradicate, persistent infections. can form on many medical devices implants, so have an enormous impact medicine. Due lack effective anti-biofilm antibiotics, novel alternative compounds or strategies urgently required. This review describes some latest approaches in field biofilm treatment. New technologies target different...

Abstract Bacterial resistance to antibiotics is increasing at an alarming rate and many commonly used are no longer effective. Thus, there considerable interest in investigating novel antibacterial compounds, such as the plant-derived pentacyclic triterpenoids, including oleanolic acid (OA), ursolic (UA) their derivatives. These compounds can be isolated from medicinal crop plants antibacterial, antiviral, antiulcer anti-inflammatory effects well documented. OA UA active against bacterial...

Studies on new antibacterial therapeutics and strategies are currently being conducted in many microbiological, pharmaceutical biochemical laboratories. The activity of plant-derived compounds as well silver gold nanoparticles is the subject this minireview. application photodynamic therapy also discussed.

Selenium is an essential trace element for the proper functioning of human body. In recent years, great attention has been paid to selenium nanoparticles (SeNPs) due their potential medicinal applications. this study, herbal extracts were used in green synthesis SeNPs. The influence species, ratio reagents, and post-reaction heating on antibacterial antioxidant properties obtained SeNPs investigated. relationship between these physical parameters (e.g., size, shape) was also studied. It...

The antibacterial and antiparasitic activities of free oleanolic acid its glucosides glucuronides isolated from marigold (Calendula officinalis) were investigated. MIC the effect on bacterial growth estimated by A600 measurements. Oleanolic acid's influence survival ability to induce autolysis measured counting number cfu. Cell morphology presence endospores observed under electron light microscopy, respectively. inhibited survival, influenced cell enhanced Gram-positive bacteria suggesting...

The objective of this study was to characterize the effects silver nanoparticles on Pseudomonas aeruginosa. Their interactions with several conventional antibiotics and ability induce a stress response were examined. Interactions between (AgNPs) against free-living cells biofilm P. aeruginosa studied using chequerboard method time-kill assays. AgNPs determined by evaluation cellular levels DnaK HtpG chaperones SDS-PAGE Western blot analysis. Synergistic activity ampicillin, streptomycin,...

Background: Selenium nanoparticles (SeNPs) show high therapeutic potential. SeNPs obtained by green synthesis methods, using commonly available plants, are an attractive alternative to classical, chemical methods. The process uses environmentally friendly reagents, which offer eco-friendly advantage. Clarifying their mechanism of action is key safe use. Methods: study used extracts sage, hops, blackberry, raspberry, and lemon balm, without the use additional stabilizers, chemically with...

Aim: To investigate the in vitro activity of silver NPs (AgNPs) against pathogenic microalgae Prototheca genus. Materials & methods: The antialgal potential AgNPs species both clinical and environmental origin was assessed from minimum inhibitory (algistatic) algicidal concentrations. cytotoxicity bovine mammary epithelial cell line evaluated by means standard MTT assay.Results: showed a strong killing toward algae, as minimal concentration (MAC) values matched perfectly corresponding (MIC)...

Aim: Pseudomonas aeruginosa is one of the most clinically important opportunistic pathogen in humans. The aim project was to study effects HtpG on selected virulence factors responsible for pathogenesis and biofilm formation P. aeruginosa. Methodology: By characterizing a htpG null mutant aeruginosa, we have identified role production factors. Results: We showed that ΔhtpG affects many physiological processes containing: decreased activity LasA protease, reduction formation, motility,...

The bacterial chaperone high-temperature protein G (HtpG), a member of the Hsp90 family, is involved in protection cells against variety environmental stresses. ability HtpG to form complexes with other proteins, especially those fundamental functions, indicative its cellular role. An interaction between and DnaA, main initiator DNA replication, was studied both vivo, using two-hybrid system, vitro modified pull-down assay by chemical cross-linking. In this demonstrated only when htpG...

The application of chicken waste to farmland could be detrimental public health. It may contribute the dissemination antibiotic-resistance genes (ARGs) and antibiotic-resistant bacteria (ARB) from feces their subsequent entry into food chain. present study analyzes metagenome resistome manure litter obtained a commercial farm in Poland. ARB were isolated, identified, screened for antibiogram fingerprints using standard microbiological molecular methods. physicochemical properties also...

Oleanolic acid and ursolic are pentacyclic triterpenoids isolated from a variety of medicinal plants, which have antibacterial activity. Listeria monocytogenes is Gram-positive facultative pathogen, being the causative agent listeriosis. The present study was carried out to evaluate in vitro effect sub-inhibitory concentrations both triterpene acids on pathogenicity determinants L. monocytogenes: their hemolytic activity biofilm forming ability. inhibited listeriolysin O without influencing...

Abstract Listeria monocytogenes is Gram-positive bacterial pathogen, a causative agent of food poisoning and systemic disease – listeriosis. This species still susceptible to several conventionally used antibiotics but an increase in its resistance has been reported. For this reason the search for new, alternative therapies urgent task. Silver nanoparticles seem be promising antibacterial agent. Minimal inhibitory concentration silver was determined. Sublethal concentrations were study...

DnaJ chaperone, a member of the so called DnaK-DnaJ-GrpE chaperone machine plays an important role in cell physiology. The ability Escherichia coli ΔdnaJ mutant to form biofilm was studied. It shown that this is impaired development when exposed 42 degrees C for 2 h. impairment observed heat shock applied either at onset formation or h later. formed thinner and its structure changed as compared wild-type strain. This defect could be complemented by introduction gene on low-copy plasmid.

The frequency of spontaneous as well induced reversions auxotrophic mutations in Escherichia coli AB1157 and its ΔdnaK ΔdnaKdnaJ derivatives was estimated. obtained results demonstrate that both mutants tested are characterized by elevated compared to their parent. In contrast, the UV MMS, i.e. agents inducing SOS response, is reduced ΔdnaJ mutants, pointing possible defect these error prone repair. Due fact UmuC protein one main players executing repair, stability also studied. Reduced...