Due to the risk of pathogenic antibiotic-resistant bacteria and their antibiotic-resistance genes transfer from livestock feces soil cultivated crops, it is imperative find effective on-farm manure treatments minimize that hazardous potential. An introduced worldwide policy sustainable development, focus on ecological agricultural production, circular economy aimed at reducing use artificial fertilizers; therefore, such treatment methods should also maximize fertilization value animal...

Manure from food-producing animals, rich in antibiotic-resistant bacteria and antibiotic resistance genes (ARGs), poses significant environmental healthcare risks. Despite global efforts, most manure is not adequately processed before use on fields, escalating the spread of antimicrobial resistance. This study examined how different cattle treatments, including composting storage, affect its microbiome resistome. The changes occurring resistome treated samples were compared with those raw by...

The aim of this study was to analyze the resistance Staphylococcus aureus isolates from bovine mastitis in eastern part Poland a set 20 antibiotics and three alternative agents: lysostaphin, nisin polymyxin B. Eighty-six out 123 examined were susceptible all tested (70%). highest percentage observed case β-lactam antibiotics: amoxicillin (n=22, 17.9%), ampicillin (n=28, 22.8%), penicillin (n=29, 23.6%) streptomycin (n=13; 10.6%). Twenty-five penicillin-resistant strains found carry blaZ gene...

Comprehensive whole-genome sequencing was performed on two multi-drug-resistant

Abstract Antibiotic therapy of staphylococcal mastitis is characterized by significantly lower cure rates compared to infections caused other microorganisms. Thus, it necessary search for new, alternative, non-antibiotic agents that are effective in the eradication these bacteria. The aim our research was investigate antimicrobial, especially anti-staphylococcal potential a large collection (n=36) essential oils (EOs). Investigation antimicrobial activity tested determined using serial,...

Abstract MicroRNAs (miRNAs) are short, non-coding RNAs, 21–23 nucleotides in length which known to regulate biological processes that greatly impact immune system activity. The aim of the study was compare miRNA expression non-infected (H) mammary gland parenchyma samples with glands infected coagulase-positive staphylococci (CoPS) or coagulase-negative (CoNS) using next-generation sequencing. profile found change during mastitis, its depending on type pathogen. Comparing CoPS and H groups,...

The application of chicken waste to farmland could be detrimental public health. It may contribute the dissemination antibiotic-resistance genes (ARGs) and antibiotic-resistant bacteria (ARB) from feces their subsequent entry into food chain. present study analyzes metagenome resistome manure litter obtained a commercial farm in Poland. ARB were isolated, identified, screened for antibiogram fingerprints using standard microbiological molecular methods. physicochemical properties also...

Lysostaphin is an enzyme with bactericidal activity against Staphylococcus aureus and other staphylococcal species. In spite of many advantages promising results preliminary research, the still not widely used in medicine, veterinary or as a food preservative. One most important factors limiting application clinical technological practice high cost its production. this study we have determined optimal conditions for lysostaphin production 5-L batch bioreactor. The was based on heterologous,...

The aim of the study was to analyze acute phase protein and cathelicidin gene responses small ruminant lentivirus (SRLV) infection in goats. In uninfected goats, we found higher Cp lower Fbγ mRNA levels blood leucocytes (BL) than milk somatic cells (MSC), as well SAA, Hp, CRP AGP concentrations serum milk. SRLV-infected MAP28 expression MSC BL, Fb, serum. Higher SAA Hp expressions BL were observed a concentration serum, while milk, Cp, MAP34 observed. profiles studied genes differed between...

The study aim was to determine the expression of genes potentially related chronic mastitis at mRNA and protein levels, viz. chemokine C-C motif receptor 1 (CCR1), ligand 2 (CCL2), 5 (CXCL5), tumor necrosis factor α (TNFα), interleukin 1β (IL-1β), 6 (IL-6), 8 (IL-8), 18 (IL-18), in bovine mammary gland parenchyma. examines differences selected between cows with caused by coagulase-positive (CoPS) or coagulase-negative staphylococci (CoNS) those healthy udders (H). Samples were collected from...

<title>Abstract</title> The study describes the whole-genome sequencing of two antibiotic-resistant representative <italic>Escherichia coli</italic> strains, isolated from poultry manure in 2020. samples were obtained a commercial chicken meat production facility Poland. antibiotic resistance profile was characterized by co-resistance to β-lactam antibiotics, aminoglycosides, and fluoroquinolones. three identified plasmids (R-plasmids), pECmdr13.2, pECmdr13.3 pECmdr14.1, harbored various...

This study involved 45 Holstein and 60 Holstein-Charolaise steers, tailored with specific diets according to breed rearing systems. DNA genotyping was conducted for DGAT1, LEP, SCD1, SREBF1, TG genes investigate their impact on carcass conformation traits, beef quality sensory traits. The results showed associations between the genetic variants analyzed Specifically, DGAT1 found affect drip loss, meat brightness, color saturation. gene associated marbling color. LEP influenced trim fat pH...

The study describes the whole-genome sequencing of two antibiotic-resistant representative Escherichia coli strains, isolated from poultry manure in 2020. samples were obtained a commercial chicken meat production facility Poland. antibiotic resistance profile was characterized by co-resistance to β-lactam antibiotics, aminoglycosides, and fluoroquinolones. three identified plasmids (R-plasmids), pECmdr13.2, pECmdr13.3, pECmdr14.1, harbored various genes conferring tetracyclines (tetR[A])...

Mastitis is one of the most common diseases in dairy cattle. It significantly reduces milk quality and yield, thus incurring economic losses for farmers. This study investigates impact various bacterial pathogens on somatic cell count, composition, technological properties samples from 302 clinically healthy Polish Holstein-Friesian cows kept under intensive rearing conditions. Of 462 analyzed, 85.06% were contaminated with bacteria. The majority coagulase-negative staphylococci...

Abstract Background Mastitis is the most common disease in dairy cattle and costliest for farming industry, as it lowers milk yield quality. occurs a result of interactions between microorganisms individual genetic predispositions each animal. Thus, important to fully understand mechanisms underlying these interactions. Elucidating immune response can determine which background makes an animal highly resistant mastitis. We analyzed innate responses cows naturally infected with...

Abstract Manure from food-producing animals, rich in antibiotic-resistant bacteria and antibiotic resistance genes (ARGs), poses significant environmental healthcare risks. Despite global efforts, most manure is not adequately processed before use on fields, escalating the spread of antimicrobial resistance. This study examined how different cattle treatments, including composting storage, affect its microbiome resistome. The changes occurring resistome treated samples were compared with...

A collection of 206 Staphylococcus spp. isolates was investigated for their ability to produce compounds exhibiting antistaphylococcal activity. This group included aureus, epidermidis, xylosus strains recovered from bovine mastitis (n = 158) and human skin wounds soft tissues infections 48). Production substances with antimicrobial activity observed in six strains. Five them were mastitis, one isolated the infected wound. Three antimicrobials produced by different showed substantial loss...