A5047335035- Plant Reproductive Biology
- Plant Molecular Biology Research
- Polysaccharides and Plant Cell Walls
- Photosynthetic Processes and Mechanisms
- Plant Taxonomy and Phylogenetics
- Stress Responses and Cortisol
- Grief, Bereavement, and Mental Health
- Molecular Biology Techniques and Applications
- Suicide and Self-Harm Studies
- Plant Stress Responses and Tolerance
- Tryptophan and brain disorders
- COVID-19 and Mental Health
Universidade do Porto
2016-2024
Rede de Química e Tecnologia
2020-2024
John Innes Centre
2024
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2023
American Academy of Forensic Sciences
2021
Hospital de São João
2020
The University of Adelaide
2020
In higher plants, the female germline is formed from megaspore mother cell (MMC), a single in premeiotic ovule. Previously, it was reported that mutants RNA-dependent DNA methylation (RdDM) pathway might be involved restricting to nucellus cell. We show DRM methyltransferase double mutant drm1drm2 also presents ectopic enlarged cells, consistent with supernumerary MMC-like cells. wild-type ovules, MMC differentiation requires SPOROCYTELESS/NOZZLE (SPL/NZZ), as demonstrated by spl/nzz failing...
The fasciclin-like arabinogalactan proteins organization into four groups is conserved and may be related to specific roles in developmental processes across angiosperms. Fasciclin-like (FLAs) are a subclass of (AGPs), which contain domains addition typical AGP domains. FLAs present all embryophytes, despite their low overall sequence similarity, regions that define the fasciclin functional domain (FAS) have been identified, suggesting cell adhesion property also conserved. Arabidopsis...
Quantitative real-time polymerase chain reaction (qPCR) is a widely used method to analyse the gene expression pattern in reproductive tissues along with detecting levels mutant backgrounds. This technique requires stable reference genes normalise level of target genes. Nonetheless, considerable number publications continue present qPCR results normalised single and, our knowledge, no comparative evaluation multiple has been carried out specific Arabidopsis thaliana. Herein, we assessed...
Cell size affects many processes, including exchange of nutrients and external signals, cell division tissue mechanics. Across eukaryotes, cells have evolved mechanisms that assess their own to inform processes such as cycle progression or gene expression. Here, we review recent progress in understanding plant regulation its implications, relating these findings work other eukaryotes. Highlights include use DNA contents reference point control the shoot meristems, a size-dependent fate...
Abstract Correct regulation of intercellular communication is a fundamental requirement for cell differentiation. In Arabidopsis thaliana , the female germline differentiates from single somatic ovule that becomes encased in β−1,3-glucan, water insoluble polysaccharide implicated limiting pathogen invasion, regulating trafficking roots, and promoting pollen development. Whether β−1,3-glucan facilitates isolation development has remained contentious, since limited evidence available to...
Inflammation has been implicated in core features of depression pathophysiology and treatment resistance. Therefore, new challenges the discovery inflammatory mediators have emerged. MicroRNAs (miRNAs) found aberrantly expressed several pathologies, increasing their potential as biomarkers therapeutical targets. In this study, aim was to assess changes biomarker inflammation-related miRNAs patients.Depression diagnosis performed according Diagnostic Statistical Manual Mental Disorders, Fifth...
Eukaryotic cells rely on the accuracy and efficiency of vesicular traffic. In plants, disturbances in trafficking are well studied quickly dividing root meristem or polar growing hairs pollen tubes. The development female gametophyte, a unique haploid reproductive structure located ovule, has received far less attention studies transport. Key molecules providing specificity vesicle formation its subsequent recognition fusion with acceptor membrane Rab proteins. Rabs anchored to membranes by...
Arabinogalactan proteins (AGPs) are hydroxyproline-rich glycoproteins containing a high proportion of carbohydrates, widely distributed in the plant kingdom and ubiquitously present land plants. AGPs have long been suggested to play important roles reproduction there is already evidence that specific essential for male female gametophyte development, pollen tube growth guidance, successful fertilization. However, functions many these yet be uncovered, mainly due difficulty study individual...