To elucidate the role of protein conformation at air/water interface, we measured interfacial dilatational rheology bovine serum albumin (BSA) and β-casein adsorbed over long time periods using a modified dynamic pendant-drop tensiometer. Companion long-time surface pressure ellipsometry measurements are also reported. BSA has well-characterized structural isomers (conformers) whose transitions depend on solution pH. It is, therefore, possible to establish connection between structure...

Catharanthus roseus produces low levels of two dimeric terpenoid indole alkaloids, vinblastine and vincristine, which are widely used in cancer chemotherapy. The dimerization reaction leading to α-3′,4′-anhydrovinblastine is a key regulatory step for the production anticancer alkaloids planta has potential application industrial semisynthetic derivatives also as drugs. In this work, we report cloning, characterization, subcellular localization an enzyme with anhydrovinblastine synthase...

Some of the most important changes that occur in plants during sexual reproduction involve transition from a sporophytic to gametophytic type development. In this paper, these were evaluated for Arabidopsis thaliana. The results obtained clearly show differences pattern distribution specific arabinogalactan protein (AGP) sugar epitopes, anther and ovule AGPs are hydroxyproline-rich glycoproteins massively glycosylated ubiquitous plants. molecular mechanism action is still unknown, mainly due...

Arabinogalactan proteins (AGPs) are structurally complex plasma membrane and cell wall proteoglycans that implicated in diverse developmental processes, including plant sexual reproduction. Male gametogenesis (pollen grain development) is fundamental to The role of two abundant, pollen-specific AGPs, AGP6, AGP11, have been investigated here. pollen specificity these suggested they integral biogenesis their strong sequence homology indicated a potential for overlapping function. Indeed,...

Arabinogalactan proteins (AGPs) are cell wall proteoglycans that have been shown to be important for pollen development. An Arabidopsis double null mutant two pollen-specific AGPs (agp6 agp11) showed reduced tube growth and compromised response germination cues in vivo. A microarray experiment was performed on agp6 agp11 tubes search genetic interactions the context of growth. yeast two-hybrid AGP6 AGP11 also designed.The lack specific induced a meaningful shift gene expression profile. In...

The fasciclin-like arabinogalactan proteins organization into four groups is conserved and may be related to specific roles in developmental processes across angiosperms. Fasciclin-like (FLAs) are a subclass of (AGPs), which contain domains addition typical AGP domains. FLAs present all embryophytes, despite their low overall sequence similarity, regions that define the fasciclin functional domain (FAS) have been identified, suggesting cell adhesion property also conserved. Arabidopsis...

Surface or disjoining forces between protein layers adsorbed at the air/water interface were measured in single, isolated films. Two proteins, β-casein and bovine serum albumin (BSA), investigated under varying conditions of pH, ionic strength, degree aging interface. Force−distance curves determined with a modified thin-film balance interferometer, using both equilibrium dynamic methods. Dynamic surface tension ellipsometry data for BSA adsorption are also reported. Charged molecules do not...

Abstract Background and Aims Morphogenesis occurs through accurate interaction between essential players to generate highly specialized plant organs. Fruit structure function are triggered by a neat transcriptional control involving distinct regulator genes encoding transcription factors (TFs) or signalling proteins, such as the C2H2/C2HC zinc-finger NO TRANSMITTING TRACT (NTT) MADS-box protein SEEDSTICK (STK), which important in setting reproductive competence, feasibly affecting cell wall...

Some AGP molecules or their sugar moieties are probably related to the guidance of pollen tube into embryo sac, in final part its pathway, when arriving at ovules. The specific labelling synergid cells and filiform apparatus, which responsible for attraction, also micropyle micropylar nucellus, constitutes entryway quite indicative this role. We discuss posibility that AGPs sperm involved double fertilization process.

The Brazilian city of Três Lagoas, Mato Grosso do Sul State, has experienced an urban outbreak visceral leishmaniasis since 2000. In 2002, due to the increase in number cases, 46 families with cases were studied verify prevalence asymptomatic infection household contacts. Indirect immunofluorescence and ELISA showed a 36.4% positive rate. There no symptomatic disease among these was statistically significant difference gender or age. Median age 21 years, 10-19-year bracket most heavily...

Abstract Key message GPI anchor addition is important for JAGGER localization and in vivo function. Loss of correct JAGGER, negatively affects its In flowering plants, successful double fertilization requires the delivery two sperm cells to female gametophyte inside ovule. The a single pair achieved by entrance pollen tube into one gametophyte. To prevent polyspermy, Arabidopsis ovules avoid attraction multiple tubes ovule–polytubey block. jagger mutants, significant number attract more than...

Abstract A kinetic framework is developed to describe enzyme activity and stability in two‐phase liquid–liquid systems. In particular, the model applied enzymatic production of benzaldehyde from mandelonitrile by Prunus amygdalus hydroxynitrile lyase (pa‐Hnl) adsorbed at diisopropyl ether (DIPE)/aqueous buffer interface (pH = 5.5). We quantitatively our previously obtained experimental results (Hickel et al., 1999; 2001), we successfully account for aqueous‐phase concentration dependence...

Arabinogalactan proteins (AGPs) are hydroxyproline-rich glycoproteins containing a high proportion of carbohydrates, widely distributed in the plant kingdom and ubiquitously present land plants. AGPs have long been suggested to play important roles reproduction there is already evidence that specific essential for male female gametophyte development, pollen tube growth guidance, successful fertilization. However, functions many these yet be uncovered, mainly due difficulty study individual...

Following studies on the transcriptome of pollen tubes an agp6 agp11 Arabidopsis double null mutant, together with knowledge that arabinogalactan protein (AGP) 6 is important for male gametogenesis and germination, we sought to know whether AGP6 could be present in vegetative cell wall or generative both. To end, plants were transformed gene sequence fused Sirius fluorescent protein. Fluorescent blue light emission detected only. This result supports model by which other AGPs are probably...

ABSTRACT In flowering plants, successful double fertilization requires the correct delivery of two sperm cells to female gametophyte inside ovule. The a single pair is achieved by entrance pollen tube into one gametophyte. To prevent polyspermy, Arabidopsis ovules avoid attraction multiples tubes ovule – polytubey block. jagger mutants, significant number attract more than an due impairment in synergid degeneration. JAGGER encodes putative arabinogalactan protein (AGP) which predicted be...