A5066900416- Clostridium difficile and Clostridium perfringens research
- Microscopic Colitis
- Viral gastroenteritis research and epidemiology
- Gut microbiota and health
- Nosocomial Infections in ICU
- Antimicrobial Resistance in Staphylococcus
- Helicobacter pylori-related gastroenterology studies
- Respiratory and Cough-Related Research
- Probiotics and Fermented Foods
- Inhalation and Respiratory Drug Delivery
- Herbal Medicine Research Studies
- Antibiotic Use and Resistance
- Gastrointestinal motility and disorders
- Bacterial Identification and Susceptibility Testing
- Photodynamic Therapy Research Studies
- Bacteriophages and microbial interactions
- Medical and Biological Ozone Research
- Hydrogen's biological and therapeutic effects
- Pharmacological Effects of Natural Compounds
- Pneumonia and Respiratory Infections
- Enterobacteriaceae and Cronobacter Research
- Infant Health and Development
- Healthcare cost, quality, practices
- Infant Nutrition and Health
- Pediatric health and respiratory diseases
Medical University of Warsaw
2013-2024
University of Warsaw
2010
As part of the European Clostridium difficile infections (CDI) surveillance Network (ECDIS-Net), which aims to build capacity for CDI in Europe, we constructed a new network hospital-based laboratories Poland. We performed survey 13 randomly selected hospital-laboratories different sites country determine their annual incidence rates from 2011 2013. Information on C. laboratory diagnostic testing and indications was also collected. Moreover, 2012 2013 respectively, participating sent all...
We aimed to describe the clinical presentation, treatment, outcome and report on factors associated with mortality over a 90-day period in Clostridioides difficile infection (CDI). Descriptive, univariate, multivariate regression analyses were performed data collected retrospective case-control study conducted nine hospitals from seven European countries. A total of 624 patients included, which 415 deceased (cases) 209 still alive 90 days after CDI diagnosis (controls). The most common...
The prevalence of Clostridium difficile infection (CDI) in pediatric patients with inflammatory bowel disease (IBD) is still not sufficiently recognized. We assessed the CDI and recurrences outpatients IBD. In addition, influence IBD therapy on antimicrobial susceptibility potentially causative C. strains was assessed. This a prospective, single-center, observational study. All specimens were obtained between January 2005 2007 from outpatient service screened for its toxins. isolates...
Clostridium difficile infection (CDI) is a major cause of nosocomial diarrhea. CDI known to develop after antibiotic administration, but anti-tuberculosis agents have rarely been implicated. We documented an outbreak caused by highly rifampicin-resistant C. strain polymerase chain reaction (PCR) ribotype 046 in patients with active tuberculosis.
The aim of this study was to investigate the effects that prebiotic and candidates for prebiotics on Clostridium difficile strains adhere various human epithelial cell lines compare adhesive properties specific C. strains. We also sought examine effect different concentrations fructooligosaccharides mannose formation biofilms by influence cellobiose, fructooligosaccharides, inulin, mannose, raffinose adherence strains, including motile 630, non-motile M120, 10 clinical ribotype 027...
Isolates (79 in total) of Clostridium difficile obtained over a 2 year period from 785 patients suspected having C. difficile-associated diarrhoea (CDAD) and being hospitalized the University Hospital Warsaw were characterized by toxigenicity profile PCR ribotyping. Furthermore, their susceptibility to clindamycin erythromycin was determined. Among 79 isolates, 35 classified as (A+)B+, 1 (A+)(B+)CDT+, 36 (A-)B+ 7 (A-)B-. A total 21 different ribotypes detected. Two main (A+)B+ strains...
Abstract This study aims to investigate the antimicrobial and antibiofilm activity of berberine chloride (BBR) vancomycin (VAN) as well synergistic combinations BBR with VAN against Clostridioides difficile strains. The effect different concentrations on strain motility was also assessed. Twelve C. strains (two reference 630, ATCC 9689, one control M120, 9 clinical belonging PCR-ribotype (RT027)) were collected investigated for their susceptibility in planktonic biofilm forms. Both minimum...
Aim: The objective of this study was to determine the effect standard and candidate prebiotics on adhesion biofilm formation Bacteroides sp. in monoculture co-culture with Clostridioides difficile. Materials & methods: seven three human cell lines determined. fructooligosaccharides (FOS) C. difficile tested by co-incubation assay. Results: Inulin, mannose raffinose presented best anti-adhesion properties against Combination FOS decreased Conclusion: shows potential role synbiotics decreasing...
Bacterial adhesion is the first stage of colonisation and biofilm formation by Clostridioides difficile. Cell wall proteins (Cwp) 84 66 play crucial roles in pathophysiology C. difficile may affect bacterial adhesion. Sialylated human milk oligosaccharides (HMOs) have potential to inhibit vitro. The aim this study was investigate how 3'-sialyllactose (SL) 6'-SL formation. Also, influence these substances on cwp84 cwp66 genes expression assessed. An assay performed using three colon cells...
Understanding the potential antimicrobial properties of natural compounds and their impacts on Clostridioides difficile virulence factors may aid in developing alternative strategies for preventing treating C. infections (CDI). In this study, we investigated bactericidal effects ginger oil (GO), peppermint (PO), curcumin (CU), cinnamon aldehyde (CI), trans-cinnamaldehyde (TCI) adhesion biofilm disruption difficile. We used three reference five clinical strains different ribotypes. The...
This study analysed 330 Clostridium difficile strains isolated from patients with C. infection who were hospitalized in two university hospitals (H1 and H2) Warsaw, Poland, over the period 2004-2006. Strains investigated for presence of tcdA (A), tcdB (B) binary toxin (CDT) genes, antimicrobial susceptibility was determined against nine agents. Among isolates, 150 (45.4 %) classified as A(+)B(+)CDT(-), 18 (5.5 A(+)B(+)CDT(+), 144 (43.6 A(-)B(+)CDT(-) A(-)B(-)CDT(-). The predominant PCR...
ABSTRACT Clinically relevant Clostridium difficile strains usually produce toxins A and B. Some C. can an additional binary toxin. We report clonality among five carrying all toxin genes from Polish patients with -associated diarrhea. In another strain, possible recombination between is documented.
Amplified fragment length polymorphism genotypes, antibiotic resistance profiles, and toxin profiles of Clostridium difficile strains from Warsaw were determined. The isolates segregate in six major coinciding with profiles. Most the A-negative B-positive CDT-negative possess ermB, several resistant to fluoroquinolones. Resistograms types C. are epidemicity determinants.
There is an ongoing search for alternative treatments Clostridioides difficile infections. The aim of the study was to investigate antibacterial and antibiotic activity bee products against C. strains with different polymerase chain reaction ribotypes (RTs). minimum inhibitory concentration (MICs) Manuka honey 550+, goldenrod honey, pine bread were determined by broth dilution method. adhesion HT-29, HT-29 MTX, CCD 841 CoN cell lines assessed. Biofilm cultured in titration plates visualized...
The motility and genotype of the flagellin fliC fliD genes were investigated in 82 Clostridioides difficile isolates belonging to ribotypes (RTs): 027 (n = 41), 176 17), 023 8), 017 6) 046 10). reference C. difficile strains 630 M120 included as controls for assay.A Multiple Locus Variable-number Tandem Repeat Analysis (MLVA) was used exclude genetic relatedness same RT. variability fliC determined by PCR-restriction fragment length polymorphism (RFLP) analysis Sanger sequencing. assay carried...
is a predominant nosocomial pathogen within the healthcare setting able to produce biofilms. Sub-minimum inhibitory concentrations (sub-MICs) of antibiotics trigger mechanisms affecting bacterial virulence, including increased adhesion and biofilm formation. The aim this study was investigate how sub-MICs metronidazole affect formation
Diarrhea is a common problem in nursing homes. A survey among facilities Poland was used to characterize diarrhea outbreaks, the burden caused for residents and caregivers employed measures. Survey results confirmed that homes most cases affects groups of residents. The related high or very 27% 40% caregivers. In 80% pro synbiotics are part measures manage diarrhea. Administration these kinds products has been suggested management diarrhea, especially by