A5043646878- Clostridium difficile and Clostridium perfringens research
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Viral gastroenteritis research and epidemiology
- Bacterial biofilms and quorum sensing
- Helicobacter pylori-related gastroenterology studies
- Enterobacteriaceae and Cronobacter Research
- SARS-CoV-2 and COVID-19 Research
- Microscopic Colitis
- Pharmaceutical and Antibiotic Environmental Impacts
- Herbal Medicine Research Studies
- Antibiotic Use and Resistance
- Diverticular Disease and Complications
- Gut microbiota and health
- SARS-CoV-2 detection and testing
- Antifungal resistance and susceptibility
Charles University
2020-2025
University Hospital in Motol
2021
Public transport represents a potential site for the transmission of resistant pathogens due to rapid movement large numbers people. This study aimed investigate bacterial contamination frequently touched surfaces in public system operating proximity biggest Czech hospital during coronavirus pandemic despite extensive cleaning and disinfection efforts. In June September 2020, samples from metro trains, ground stationary objects were collected, enriched cultured. The antimicrobial...
Clostridioides difficile is one of the most important human pathogens. The identification its possible sources for understanding C. infection (CDI) epidemiology. A total 16 water samples from wastewater and surface in South Moravia Czech Republic 82 fish gulls were collected between May July 2019. isolates cultured by direct plating after enrichment on chromogenic media. Susceptibility testing to eight antimicrobials was performed Etest. characterized ribotyping, multilocus sequence typing,...
Methicillin-resistant Staphylococcus aureus (MRSA) is a leading cause of health care-associated infections. Additionally, over the decades, spread community-associated (CA-MRSA) clones has become serious problem. The aim this study was to gain data on current epidemiology MRSA in Slovakia. Between January 2020 and March 2020, single-patient isolates (invasive and/or colonizing) were collected Slovakia from hospitalized inpatients (16 hospitals) or outpatients (77 cities). Isolates...
Background: In order to estimate the prevalence of plasmid borne colistin resistance and characterize in detail mcr-positive isolates, we carried out a sentinel testing survey on intestinal carriage plasmid-mediated colistin-resistant Enterobacteriaceae hospitalized patients. Methods: Between June 2018 September 2019, 1922 faecal samples from hospitalised patients were analysed by selective culture presence (3.5 mg/L), parallel direct detection mcr-1 mcr-8 genes qPCR. The isolates...
To gain data on the current molecular epidemiology and resistance of MRSA in Czech Republic.Between September 2017 January 2018, a total 441 single-patient isolates were collected from 11 hospitals analysed by spa typing, SCCmec antibiotic susceptibility testing, detection PVL toxin arcA gene.Of all isolates, 81.41% (n = 359) belonged to CC5-MRSA clone represented types t003 136), t586 92), t014 81), t002 20) other 30); majority CC5 348, 96.94%) carried type II. The occurrence was more...
To investigate the epidemiology of Clostridioides difficile infection (CDI) in Slovakian hospitals after emergence ribotype 176 (027-like) 2016.Between 2018 and 2019, European Centre for Disease Control Prevention CDI surveillance protocol v2.3 was applied to 14 hospitals, with additional data collected on recent antimicrobial use characterization C. isolates.The mean hospital incidence 4.1 cases per 10,000 patient bed-days. One hundred five (27.6%) in-hospital deaths were reported among 381...
We performed a retrospective analysis of MRSA isolates collected at the university equine clinic including clinical from 2008 to 2021 and screening environmental, personnel 2016.
The motility and genotype of the flagellin fliC fliD genes were investigated in 82 Clostridioides difficile isolates belonging to ribotypes (RTs): 027 (n = 41), 176 17), 023 8), 017 6) 046 10). reference C. difficile strains 630 M120 included as controls for assay.A Multiple Locus Variable-number Tandem Repeat Analysis (MLVA) was used exclude genetic relatedness same RT. variability fliC determined by PCR-restriction fragment length polymorphism (RFLP) analysis Sanger sequencing. assay carried...
The carriage of multidrug-resistant (MDR) pathogens in medical students has not been studied extensively, despite the fact that they are contact with patients and exposed to a hospital environment.To investigate intestinal nasal MDR among its association their lifestyle demographic data.In 2021, first- final-year were invited study. Two rectal swabs used for detection extended-spectrum β-lactamase (ESBL)-producing, colistin-, tigecycline- or carbapenem-resistant Gram-negative bacteria...
Abstract Background The rapid diagnostics tests for SARS‐CoV‐2 antigen vary in their sensitivities, and moreover, genomic mutations may further affect the performance of assays. We aimed to evaluate analytical an automated assay compare its sensitivity Delta‐ Omicron‐variant positive clinical samples. Material methods mariPOC test was evaluated on a population community‐dwelling subjects with mild respiratory symptoms or being asymptomatic investigated by RT‐qPCR Allplex™ assay. specificity...