Stenotrophomonas maltophilia is an environmental Gram-negative bacterium that has rapidly emerged as important nosocomial pathogen in hospitalized patients. Treatment of S. infections difficult due to increasing resistance multiple antibacterial agents. The purpose this study was determine the phenotypic and genotypic characterization isolates recovered from patients referred several hospitals. A total 164 clinical were collected hospitals various regions Iran between 2016 2017. Antibiotic...

: Acinetobacter baumannii (A. baumannii) is an important opportunistic pathogen responsible for nosocomial infections worldwide at recent decades. Biofilm formation by A. leads to antibiotic resistance and survives on abiotic biotic surfaces. In the present study we aimed assess ability of biofilm in clinical environmental isolates phenotypic methods detect presence genes involving development; bap, ompA, csuE, abaI, blaPER-1by PCR method. Totally 120 baumanniin isolates, 98 clinical, 22...

Background and Objectives: Colorectal cancer is one of the most types cancer. Researchers have shown that lactic acid bacteria antitumor activity. The cell wall Lactococcus lactis, as bacterial cytoplasmic extract nisin can affect proliferation cells. Since cyclin D1 plays an important role in progression cycle, its regulation also be a therapeutic approach. We investigated antiproliferative effect wall, on SW480 line expression level gene treated Materials Methods: lines were with different...

Abstract Background This study was aimed to characterize the genetic diversity and expression of three putative resistance-nodulation-cell division (RND)-type efflux systems their contribution multidrug in clinical isolates Acinetobacter baumannii . Methods Antimicrobial susceptibility testing 95 A. determined by Kirby-Bauer disk diffusion for 18 antibiotics minimum inhibitory concentration (MIC) colistin broth microdilution method. Moreover, MIC five classes assessed using E-test strips...

Abstract Objective The purpose of the present study was to investigate antimicrobial susceptibility pattern, biofilm production, and presence genes among S. maltophilia clinical isolates. A total 85 isolates were collected from patients referred several hospitals. Susceptibility antibiotics investigated by disc diffusion method according guidelines Clinical Laboratory Standards Institute (CLSI). By crystal violet staining method, capability formation examined. associated with production...

Acinetobacter baumannii is a cosmopolitan bacterium that frequently reported from hospitalized patients, especially those patients who admitted in the intensive care unit. Recently, multiplex real-time PCR has been introduced for rapid detection of resistance genes clinical isolates bacteria. The current study aimed to develop and evaluate detect common among A. baumannii. Multiplex based on melting curve analysis showed different Tm corresponding amplified fragment consisted 83.5 °C, 93.3...

Staphylococcus aureus can cause several infections. Its capability to form biofilm has been reported be a vital property involved in the bacteria's pathogenesis. Various genes contributing formation have not yet completely clarified. This study was designed evaluate factors influencing adherence and S. isolated from paediatric patients. One hundred ninety-seven isolates were obtained pediatric patients confirmed with phenotypic molecular examinations. Antimicrobial susceptibility testing...

Shigella continues to be important causes of acute pediatric diarrhea worldwide. produces numerous virulence factors involved in colonization and invasion into epithelial cells which eventually result the disease. The present study was conducted evaluate prevalence genes investigate antibiotic resistance profiles among isolates obtained from patients Iran.A total 141 were collected between March 2017 September 2018 stool children under 14 who suspected have shigellosis. identified using...

ABSTRACT Here, we described the efficacy of colistin sub-minimum inhibitory concentrations (sub-MICs) on biofilm-forming activity, host epithelial cell adherence, and invasion capacity Acinetobacter baumannii strains collected from children admitted to Children’s Medical Center Hospital. Biofilm formation potency A. clinical isolates was measured using a 96-well microtiter plate assay. Distribution biofilm-related genes, including bap , abaI ompA csuE bla PER-1 detected by PCR. The mRNA...

Background & Objective: Acinetobacter baumannii is a Gram-negative coccobacillus and one of the most opportunistic pathogens responsible for serious infections in hospitalized patients. Materials Methods: During 12 months study, 221 clinical isolates 22 environmental were collected. In vitro susceptibility to 13 antimicrobial agents: amikacin; cefepime; ceftazidime; ciprofloxacin; meropenem; piperacillin/tazobactam; sulfamethoxazole/ trimethoprim; imipenem; tigecycline; colistin; gentamycin;...

Abstract Background Community-acquired urinary tract infection (CA-UTI) could be caused by endogenous or exogenous routes. To show this relationship, we investigated molecular fingerprints and genotypes of paired Enterococcus faecalis isolated from the urine symptomatic patients their fecal samples. Results Out studied patients, 63 pairs E. isolates were obtained simultaneously feces All strains sensitive to vancomycin, linezolid, nitrofurantoin, daptomycin (MIC value: ≤ 4 µg/ml), while...

Aims The present study was conducted to investigate the mechanism of carbapenem resistance and molecular epidemiology carbapenem-resistant Acinetobacter baumannii (CRAB) isolates collected from two nearby hospitals in Tehran, Iran. Methods Results A total 180 CRAB were studied. Antimicrobial susceptibility testing performed using disk diffusion Epsilometer tests. detection OXA-23, -24 -58 implemented for all polymerase chain reaction. Subsequently, harbouring OXA-24 investigated presence...

Shigellosis is a self-limiting disease that antibiotic therapy could decrease its complications and duration. However, sublethal levels of antibiotics, may lead to alteration in state, besides role the emergence resistant variants. To understand this link, we investigated diversity Shigella serogroups children with diarrhea, S. flexneri serotypes, cytotoxic potential, resistance patterns transcriptional expression main virulence genes response sub-inhibitory concentrations azithromycin...

Cystic fibrosis (CF) is a genetic disease with high rate of morbidity and mortality. Children CF commonly suffer from recurrent persistent pulmonary tract infections caused by diverse bacterial pathogens. This study aimed to investigate the prevalence, antimicrobial susceptibility, biofilm formation isolates in pediatric patients CF. The population this cross-sectional included 8,908 children suspected have clinical manifestations March 2015 August 2017 who were referred Tehran Pediatric...

Diagnosis of active Helicobacter pylori infection in intellectually disabled (ID) children is problematic because they are unable to cooperate with performance invasive tests. In this study, the non-invasive methods measuring serum IgG antibody concentrations and performing stool antigen tests were used screen for H. ID children. Eighty-seven intellectual disabilities studied. The amount against was measured by ELISA method. Stool samples examined using an amplified IDEIA HpStAR kit. To...