Due to the rapid spread and increasing number of coronavirus disease 19 (COVID-19) cases caused by a new coronavirus, severe acute respiratory syndrome 2 (SARS-CoV-2), accurat...

Abstract Objectives Pseudomonas aeruginosa is known as a leading cause of nosocomial infections worldwide. Antimicrobial resistance and biofilm production, two main virulence factors P. , are responsible for the persistence prolonged infections. In this study, antimicrobial susceptibility pattern phenotypic genotypic characteristics were investigated. Results A total 80 clinical isolates obtained. Isolates showed to all antibiotics with rate from 12.5% (n = 10) against amikacin...

To evaluated the PCR for mecA gene compared with conventional oxacillin disk diffusion method methicillin-resistant Staphylococcus aureus (S. aureus) identification. A total of 292 S. strains were isolated from various clinical specimens obtained hospitalized patients. Susceptibility test to several antimicrobial agents was performed by agar according Clinical and Laboratory Standards Institute guidelines. The amplification carried out in all isolates. Among antibiotics used our study,...

In this study, we report the insertion sequence ISPpu21 in oprD porin gene of carbapenem-resistant Pseudomonas aeruginosa isolates from burn patients Tehran, Iran. Antibiotic susceptibility tests for P. were determined. Production metallo-β-lactamases (MBLs) and carbapenemase was evaluated β-lactamase-encoding aminoglycoside-modifying enzyme genes investigated by PCR sequencing methods. The mRNA transcription level mex efflux pump real-time PCR. outer membrane protein profile determined...

Stenotrophomonas maltophilia is an environmental Gram-negative bacterium that has rapidly emerged as important nosocomial pathogen in hospitalized patients. Treatment of S. infections difficult due to increasing resistance multiple antibacterial agents. The purpose this study was determine the phenotypic and genotypic characterization isolates recovered from patients referred several hospitals. A total 164 clinical were collected hospitals various regions Iran between 2016 2017. Antibiotic...

Abstract Background We evaluated the distribution of carbapenem and colistin resistance mechanisms clinical E. coli K. pneumoniae isolates from Iran. Methods 165 non-duplicate non-consecutive were collected hospitalized patients admitted to Iran's tertiary care hospitals September 2016 August 2018. The cultured different specimens, including wound, urine, blood, tracheal aspirates. Antibiotic susceptibility testing was performed by disc diffusion microdilution method according Clinical...

Acinetobacter baumannii is an important human pathogen with increasing notoriety in the recent years, as a causative organism of drug resistant nosocomial infections, particularly immunocompromised patients hospitalized burn centers.The aim this study was to determinate role efflux pump(s) ciprofloxacin resistance A. strains isolated from patients.Sixty-five were Motahari Burns and Reconstruction Center Tehran, Iran. Susceptibility test carried out by disk agar diffusion dilution methods,...

Background & objectives: Overexpression of efflux pumps is a cause acquired resistance to fluoroquinolones in Acinetobacter baumannii . The present study was done investigate the presence and overexpression AdeABC system analyze sequences AdeR-AdeS regulatory ciprofloxacin-resistant A. isolates. Methods: Susceptibility 50 clinical isolates ciprofloxacin, imipenem, ceftazidime, cefepime gentamicin antimicrobials evaluated by agar dilution method. Isolates were screened for evidence active...

Biofilms are a main pathogenicity feature of Pseudomonas aeruginosa and has significant role in antibiotic resistance persistent infections humans. We investigated the vitro activities ceftazidime enzyme cellulase, either alone or combination against biofilms P. aeruginosa.Both cellulase significantly decreased biofilm formation all strains dose-dependent manner. Combination at concentrations 1.25, 2.5, 5, 10 U/mL tested with 1/16× MIC led to reduction biomass. Cellulase showed detachment...

Reduced biocide susceptibility in Staphylococci is associated with various antiseptic resistance genes encoding efflux systems. Our aim was to determine the three disinfectant agents, including benzalkonium chloride (BAC), benzethonium (BZT), and chlorhexidine digluconate (CHDG) among clinical isolates of Staphylococcus aureus coagulase-negative (CoNS).The minimum inhibitory concentration (MIC) 60 methicillin-resistant S. (MRSA), 54 methicillin-sensitive (MSSA) 51 CoNS from a single hospital...

Acinetobacter baumannii is one of the most important pathogens capable colonization in burn patients, leading to drug-resistant wound infections. This study evaluated distribution AdeABC efflux system genes and their relationship ciprofloxacin resistance A. isolates collected from patients.A total 68 clinical strains were isolated patients hospitalized Motahari Burns Center Tehran, Iran. Ciprofloxacin susceptibility was tested by disk diffusion agar dilution methods. PCR amplification...

Objective: Carbapenems are effective agents to treat multidrug-resistant (MDR) strains of bacteria, including Pseudomonas aeruginosa. However, there is a potential threat emergence carbapenem-resistant P. aeruginosa (CRPA). The aim this study was determine antibiotic susceptibility patterns and metallo-beta-lactamase (MBL)-mediated resistance in clinical isolates. Materials Methods: Different specimens were subjected conventional culture-based identification Antimicrobial MBL production...

Abstract Objective The purpose of the present study was to investigate antimicrobial susceptibility pattern, biofilm production, and presence genes among S. maltophilia clinical isolates. A total 85 isolates were collected from patients referred several hospitals. Susceptibility antibiotics investigated by disc diffusion method according guidelines Clinical Laboratory Standards Institute (CLSI). By crystal violet staining method, capability formation examined. associated with production...

Purpose: Acinetobacter baumannii is the most prevalent nosocomial pathogen which have been emerged in past three decades worldwide. The aim of this study was to assess distribution AdeABC efflux pump genes, associated with tetracycline resistance isolates collected from burn infection and Ventilator Associated Pneumonia (VAP). Materials Methods: Ninety-eight A. were two different hospitals Tehran, Iran. Tetracycline susceptibility testing performed by disk diffusion agar dilution methods...

Colistin resistance rates are rising globally among multidrug-resistant Gram-negative bacilli, including Acinetobacter baumannii (A. baumannii). A new type of – heteroresistance has also been reported to colistin in clinical A. isolates. This study investigated the presence carbapenem-resistant Different specimens from hospitalised patients were for baumannii. The MICs imipenem, meropenem and determined by broth microdilution. PCR was performed detect OXA-type carbapenemase genes...

Acinetobacter baumannii is one of the most frequent nosocomial pathogen capable acquiring resistance to different antimicrobials. The aim this study was investigate activity tetracycline, doxycycline and minocycline, prevalence tet(A) tet(B) determinants, role efflux pump in tetracycline among A. clinical isolates. Susceptibility 98 isolates tetracyclines evaluated by disk diffusion method. presence active investigated determination minimum inhibitory concentration (MIC) using carbonyl...

Objectives: Biofilm formation is one of the important features Staphylococcus epidermidis, particularly in nosocomial infections.We aimed to investigate biofilm production by phenotypic methods and presence ica genes S epidermidis.Methods: A total 41 epidermidis isolates were recovered from different clinical specimens.Biofilm was evaluated microtiter plate, tube method Congo red agar method.The icaA icaD investigated PCR.Validity (sensitivity specificity), metrics for test performance...