The resistance genes encoding aminoglycoside-modifying enzymes (AMEs) are now widely prevalent in different populations of Staphylococcus aureus. study aimed to determine the frequency AMEs-encoding clinical isolates S. A total 105 aureus were obtained from samples; and then identified by conventional biochemical tests. antibiotic patterns characterized agar disk diffusion method. distribution AMEs femA was determined multiplex PCR. aminoglycoside rates kanamycin, tobramycin, gentamicin,...

Infections caused by extended-spectrum β-lactamases (ESBLs) producing bacteria, including Klebsiella pneumoniae have increasingly subjected to therapeutic limitations and patients with these infections are at high risk for treatment failure, long hospital stays, health care costs, mortality. The aim of this study was screen the prevalence blaTEM,blaCTX-M blaSHV ESBL genes in K. strains isolated from nosocomial urinary tract (UTIs). During March 2016 December 2017, one hundred isolates were...

Objective: Carbapenems are effective agents to treat multidrug-resistant (MDR) strains of bacteria, including Pseudomonas aeruginosa. However, there is a potential threat emergence carbapenem-resistant P. aeruginosa (CRPA). The aim this study was determine antibiotic susceptibility patterns and metallo-beta-lactamase (MBL)-mediated resistance in clinical isolates. Materials Methods: Different specimens were subjected conventional culture-based identification Antimicrobial MBL production...

ABSTRACT Here, we described the efficacy of colistin sub-minimum inhibitory concentrations (sub-MICs) on biofilm-forming activity, host epithelial cell adherence, and invasion capacity Acinetobacter baumannii strains collected from children admitted to Children’s Medical Center Hospital. Biofilm formation potency A. clinical isolates was measured using a 96-well microtiter plate assay. Distribution biofilm-related genes, including bap , abaI ompA csuE bla PER-1 detected by PCR. The mRNA...

<b>Objective:</b> To investigate the frequency of integrase genes <i>intI1, intI2</i> and <i>intI3</i> <i>Escherichia coli</i> strains, their association with resistance to routinely used antibiotics.<br> <b>Methods:</b> A total 120 strains were collected from patients urinary tract infection in Ahvaz, Southwest Iran. Antibiotic susceptibility testing was performed. The presence intI2</i>, determined by polymerase chain reaction.<br> <b>Results:</b> disclosed highest rate ampicillin (91.7%)...

Alpha-amylase is one of the most widely used enzymes in starch industry. However, industrial application soluble alpha-amylase hampered by changes pH and temperature (adverse effects on enzyme stability) activity loss, leading to higher costs. Immobilization an efficient strategy reduce losing subsequently reduces costs this regard. Alpha-amylases are immobilized adsorption, entrapment, covalent attachment, cross-linking. A barrier immobilization large size its substrate, namely amylose...

Lysostaphin is a glycylglycine endopeptidase, secreted by Staphylococcus simulans, capable of specifically hydrolyzing pentaglycine crosslinks present in the peptidoglycan aureus cell wall. In this paper, we describe cloning and expression lysostaphin enzyme gene Bacillus subtilis WB600 host using pWB980 system. Plasmid pACK1 S. simulans was extracted alkaline lysis method. isolated PCR cloned into pTZ57R/T-Vector, then transformed Escherichia coli DH5α. The amplified fragment uncloned...

Abstract Background β-Lactam antibiotics have been broadly used for the treatment of Acinetobacter baumannii infections, resulting in development β-lactam inactivating β-lactamases. Here, we described antibiotic resistance rate, prevalence β-lactamase-encoding genes, and clonal relationships A. strains isolated from children referred to Children’s Medical Center Tehran, Iran, during 2019–2020. Methods A total 60 non-replicate isolates were recovered clinical specimens pediatric patients....

Great attention has been focused on the discovery of anti-angiogenic natural and synthetic compounds to be finally used as or at least a part treatment tumors. The marine ecosystems provide diversity in chemicals with potential being exploited medicines diseases. Several studies have investigated Ophiuroids source anti-tumor anti-metastatic organisms. Here, we described inhibitory effects an ethanolic crude extract brittle star (Ophiocoma erinaceus) angiogenesis expression level TGF-β, VEGF,...

Burkholderia mallei is the leading cause of glanders, a highly transmittable and an OIE-notifiable disease equidae. Despite importance B. mallei, little known about serodiagnosis glanders. The present study aimed to develop immunoblotting assay based on whole-cell proteome enable accurate glanders.Three farm horses were subcutaneously immunized with crude suspension (106 cfu/ml) heat-inactivated formulated incomplete Freund's adjuvant (IFA) achieve hyperimmune sera panel. immunization was...

Background . In this study, attempts were made to evaluate the frequency of high‐level gentamicin‐resistant (HLGR) and vancomycin‐resistant enterococci (VRE) prevalence antibiotic resistance profile enterococcal species isolated from pediatric patients referred Children’s Medical Center Hospital, Tehran, over five years. Materials Methods A total 404 isolates different between March 2016 2021 included in cross‐sectional study. Antimicrobial susceptibility testing was performed using standard...

Pompe disease, a rare autosomal recessive disorder, is caused by mutations in the acid α-glucosidase gene. disease congenital metabolic disorder that affects all organs, particularly striated muscle and nerve cells. Diagnosis typically confirmed through enzyme assays reveal reduced activity. Enzyme replacement therapy utilizing human an available treatment option. Timely diagnosis early stages of significantly impact effectiveness enhancing patient condition. Here, we present case with...

Phenotypic Investigation of Biofilm Formation and the Prevalence icaA icaD Genes in Staphylococcus epidermidis Isolates

Introduction: Three percent of people worldwide are infected with Hepatitis C virus (HCV). A few studies have been performed to evaluate the biochemical markers disease. In current study, were evaluated in HCV patients and control group.&#x0D; Methods: Two sex- age-matched healthy individuals (n = 100) positive included (mean age 20-75, 26.0% females 74.0% males). Biochemical markers, including liver enzymes (ALT, AST ALP), lipid profiles (cholesterol, LDL, HDL) triglyceride (TG)...

Abstract Background: Lysostaphin is a glycylglycine endopeptidase, secreted by Staphylococcus simulans , capable of specifically hydrolyzing pentaglycine crosslinks present in the peptidoglycan aureus cell wall. In this paper, we describe cloning and expression lysostaphin enzyme gene Bacillus subtilis WB600 host using pWB980 system. Results: Plasmid pACK1 S. was extracted alkaline lysis method. isolated PCR cloned into pTZ57R/T-Vector, then transformed Escherichia coli DH5α. The amplified...

Simultaneous Detection of Bovine Rotavirus (BRV) and Viral Diarrhea (BVD) virus in Diarrheic Stool Samples: A Comparative Study Molecular Serological approaches

Glanders is a serious zoonotic disease caused by Burkholderia mallei. Prevention, control, and treatment strategies of glanders are prerequisites for microbial source tracking. The present study was aimed to analyze the genomic pattern B. mallei Iranian field isolates pulsed-field gel electrophoresis (PFGE) typing.B. were aerobically cultured in nutrient broth/agar supplemented with glycerol 4% 48 h at 37°C. API 20NE identification system used biochemical characterization. Genomic DNA...