In the present study, applicability of four wide-spectrum light-emitting diodes (LEDs) emitting warm light (AP67, AP673L, G2, and NS1) was determined for micropropagation five popular ornamental plant species: Chrysanthemum × grandiflorum, Gerbera jamesonii, Heuchera hybrida, Ficus benjamina, Lamprocapnos spectabilis. Plantlets were grown in a growth room with 16-h photoperiod. The photosynthetic photon flux density set at 62–65 μmol m−2 s−1. composition media subculture timing adjusted to...

Abstract Despite the tremendous progress in breeding, novel and user-friendly techniques of plant improvement are desirable. The study aimed to analyze usefulness silver nanoparticles (AgNPs) breeding chrysanthemum: one top ornamental species. In vitro regeneration adventitious shoots from internodes chrysanthemum ‘Lilac Wonder’ was induced on modified Murashige Skoog (MS) medium supplemented with 0.6 mg L −1 6-benzylaminopurine (BAP), 2 indole-3-acetic acid (IAA) AgNPs at 0, 5, 10 20 ppm...

The rice sheath blight disease, caused by Rhizoctonia solani J.G. Kühn fungus, is a major disease of Oryza sativa L. occurring all over the world. Therefore, efforts need to be undertaken limit spread this pathogen, preferably using environmentally friendly methods. In present study, 57 fungal isolates were recovered surface sterilization technique from 120 samples collected paddy fields in Guilan province, Iran. Biological characterizations isolated taxa performed vitro, dual culture,...

In the past, members of Cactaceae family were mostly propagated by seeds or vegetatively cuttings and grafting. Seeds, however, do not guarantee genetic stability and, in case some cacti, are difficult to obtain, their germination rates low, they need be stored for a long time specific light temperature conditions. Traditional vegetative reproduction vivo, on other hand, may less efficient insofar as limited number plants can obtained. Major issues associated with traditional propagation...

The rapid urbanization and growing number of factories, human population, motor vehicles have led to a drastic increase in the concentration air pollutants. This smog is one most important disturbances city planning. Urban trees play vital role improvement quality. selection high-potential capture pollutants provides an attractive route for mitigation urban smog. current study explored purification potential four abundant trees, i.e., white mulberry (Morus alba L.), plane tree (Platanus...

Abstract This study aimed to analyze the effect of various mutagens on in vitro development, physiological activity, acclimatization efficiency, and genetic integrity Lamprocapnos spectabilis ‘Valentine’. Gold nanoparticles (AuNPs), microwaves, X-rays were used at different doses. The profiles primary secondary metabolites enzymatic activity produced plants studied. usefulness markers detection mutations species was compared. genome size L. estimated for first time. It found that addition...

Pears are among the most economically important fruits in world that grown all temperate zones. Pyrus communis L., ‘Pyrodwarf®(S)’ rootstock is one of gene sources used to improve fruit productivity, resistance, and tolerance biotic abiotic stresses. Traditional propagation P. L. time-consuming limited by a short growing season harsh winter conditions. Therefore, vitro suitable alternative. Murashige Skoog medium (MS) woody plant (WPM) supplemented with different concentrations...

The rice blast disease caused by <i>Pyricularia oryzae</i> Cavara is among the most severe plant diseases in world. utilization of natural antagonists, such as fungal endophytes, can be a good method to control spread this disease. In present study, 68 isolates were obtained from 160 samples collected Guilan province fields, Iran. Morphological and molecular characteristics used for identification these fungi. According results, <i>Alternaria citri</i> (Penz.) Mussat,...

The integration of nanoparticles (NPs) holds promising potential to bring substantial advancements plant cryopreservation, a crucial technique in biodiversity conservation. To date, little attention has been focused on using cryobiology research. This study aimed assess the effectiveness NPs enhancing efficiency cryopreservation. In-vitro-derived shoot tips bleeding heart ( Lamprocapnos spectabilis (L.) Fukuhara) ‘Gold Heart’ and ‘Valentine’ were used as material. encapsulation-vitrification...

Studying the role of nanoparticles in plant cryopreservation is essential for developing innovative methods to conserve genetic resources amid environmental challenges. This research investigated effects gold (AuNPs), silver (AgNPs), and zinc oxide (ZnONPs) on structural integrity, stability, metabolic activity cryopreserved materials with medicinal properties. Shoot tips from two bleeding heart (Lamprocapnos spectabilis (L). Fukuhara) cultivars, 'Gold Heart' 'Valentine', were using...

This study investigated the effects of pure iron oxide nanoparticles (Fe3O4 NPs), citrate-stabilized (Fe3O4CA and indole-3-acetic acid (IAA), applied at various time regimes, on germination, growth, ex vitro development chrysanthemum synthetic seeds. The genetic metabolic stability plants was also assessed. Nodal segments Chrysanthemum × morifolium /Ramat./ Hemsl. 'Richmond', with a single axillary bud, were encapsulated in 3% calcium alginate addition IAA (1 mg·L-1) and/or NPs (7.7 mg·L-1)....

ABSTRACT The in vitro propagation of chrysanthemum (Chrysanthemum × grandiflorum (Ramat.) Kitam.), one the world’s most important ornamentals, is a very well-studied topic and shows numerous strides each year. This mini-review condenses knowledge that has been published on biotechnology, especially culture wider plant science literature. In 2013 2014, were made molecular breeding, particularly anti-viral strategies, including through transgenics, our understanding flower genetics flowering...

Echinacea purpurea (L.) Moench is a plant species important for the phytopharmaceutical industry and in horticulture. Currently, there lack of standardized material with an increased content secondary metabolites purple coneflower. The following research meets expectations industry, as new selected lines coneflower were micropropagated by somatic embryogenesis. analyzed both terms main High Performance Liquid Chromatography (HPLC), well genetic stability within line distance between using...

Abstract The popularity of nanoparticles (NPs) is continuously increasing. To date, however, there has been little research on the application NPs in plant cryopreservation, i.e. storage tissues liquid nitrogen (LN). aim this study to analyze effect and evaluate usefulness gold (AuNPs) regard cryobiology studies. In vitro-derived shoot tips Lamprocapnos spectabilis ‘Valentine’ were cryopreserved with encapsulation-vitrification protocol. Gold (at 10–30 ppm concentration; 13 nm size) added...

Abstract There is little information on the in vitro tissue culture systems Lamprocapnos spectabilis (bleeding heart). The aim of this study was to analyze influence plant growth regulators (PGRs) development, quality, and physiological state vitro- grown bleeding heart “Gold Heart” “White Gold”. Single-node explants were inoculated modified MS medium (Murashige Skoog Physiol Plant 15:473–497, 1962), fortified with different auxins, which included indole-3-acetic acid (IAA),...

Novel and unique properties of nanomaterials, which are not apparent in larger-size forms the same material, encourage undertaking studies exploring multifaced effects nanomaterials on plants. The results such only scientifically relevant but, additionally, can be implemented to plant production and/or breeding. This study aimed verify applicability silver nanoparticles (AgNPs) as a mutagen chrysanthemum Chrysanthemum × grandiflorum (Ramat.) Kitam. 'Lilac Wonder' 'Richmond' leaf explants...

Studies on nanoparticles' effects plants are relevant for horticulture. This study aimed to test the influence of zinc oxide submicron particles (ZnO SMPs), nanoparticles NPs), and combined with silver (ZnO+1%Ag NPs) applied at 100 500 mg·L-1 regeneration biochemical activity adventitious shoots in Chrysanthemum × morifolium (Ramat.) Hemsl. 'UTP Burgundy Gold' Pinky Gold'. The original microwave solvothermal synthesis characteristics ZnO samples were described. Internodes cultured MS medium...

Tomato is one of the most popular vegetable crops. However, over time, species has suffered a strong genetic diversity reduction and domestication bottlenecking. This growing trend known as erosion. The human intervention on erosion intensification high severe implications future programmes management use S. lycopersicum biodiversity. wild tomato (especially accessions originating from Andes to Mesoamerica) harbour many valuable genes, which have been lost among cultivated ones. Therefore,...

Ex situ preservation of plant genetic resources is essential. Tomato one the most important vegetable crops on market. However, diversity clade limited and suffering from erosion phenomenon. Genebanks experience alleles loss regeneration small samples, drift, somaclonal variation in vitro cultures. Therefore, development more efficient ex protocols required. Storage accessions at low temperatures allows for reduction cell metabolic activity medium or even long-term preservation. Working...

Little is known about the stability of plant chimeras after exposure to liquid nitrogen. The aim this study was evaluate effect cryopreservation on cytogenetic and genetic stability, as well vegetative generative growth chrysanthemums that are a solid mutant ('Richmond') or periclinal ('Lady Orange' 'Lady Salmon'). following encapsulation-dehydration protocol shoot tip applied: 0.09 M sucrose concentration 10 µM abscisic acid during two-week preculture, followed by 4-day osmotic dehydration...