Abstract Background Nuclear DNA content in plants is commonly estimated using flow cytometry (FCM). Plant material suitable for FCM measurement should contain the majority of its cells arrested G 0 /G 1 phase cell cycle. Usually young, rapidly growing leaves are used analysis. However, some cases seeds would be more convenient because they can easily transported and analyzed without delays additional costs required to raise seedlings. Using particularly species that leaf cytosol compounds...

The presence of some secondary metabolites in the cell cytosol can cause a stoichiometric error flow cytometric estimation nuclear DNA content. There is no fully reliable method to completely eliminate effect these compounds on nuclei fluorescence, and therefore using plant organs/parts free staining inhibitors recommended. Eleven species Rosaceae with high concentrations propidium-iodide-staining were studied check possibility seeds instead leaves for genome size estimation. Despite...

Abstract This study aimed to analyze the effect of various mutagens on in vitro development, physiological activity, acclimatization efficiency, and genetic integrity Lamprocapnos spectabilis ‘Valentine’. Gold nanoparticles (AuNPs), microwaves, X-rays were used at different doses. The profiles primary secondary metabolites enzymatic activity produced plants studied. usefulness markers detection mutations species was compared. genome size L. estimated for first time. It found that addition...

Abstract One promising area in understanding the responses of plants to ongoing global climate change is adaptative effect polyploidy. This work examines whether there a coupling between distribution cytotypes and their biogeographical niche, how different niches will affect potential range. The study uses range techniques including flow cytometry, gradient niche analysis, as well modelling. In addition, climatic, edaphic habitat data was used analyse environmental patterns ranges first...

Classical mutation breeding using physical factors is a common method for ornamental crops. The aim of our study was to examine the utility ovaries excised from irradiated inflorescences Chrysanthemum × morifolium (Ramat.) as explants purposes. We studied in vitro regeneration capacity two chrysanthemum cultivars: ‘Profesor Jerzy’ and ‘Karolina’ preceded by irradiation with high-energy photons (total dose 5, 10 15 Gy) electrons Gy). Growth inflorescence parameters greenhouse acclimatized...

The deception strategies of orchids remain poorly understood, especially in regard to the chemical compounds emitted from their flowers and interaction with various taxonomic groups pollinators. We investigated phylogenetic relationships compared variation floral between food-deceptive Dactylorhiza taxa (D. incarnata var. D. ochroleuca, fuchsii majalis) populations north-eastern Poland. propose a model evolution based on signals this genus. A Bayesian approach polymorphic plastid DNA (trnL,...

Chrysanthemum (Chrysanthemum × grandiflorum /Ramat./Kitam.) is the second most popular ornamental plant in global flower industry, and there still a demand for novelty, which forces breeders to search new sources of variation. The aim present study was evaluation phenotypic as well genetic variation chrysanthemum plants regenerated from ovaries vitro. In first vegetative season evaluated glasshouse, nine variants (16.36%) 46 (83.6%) true-totype were observed. included variegated,...

The Malva genus contains species that reveal therapeutic properties and are mostly important in medicine the functional food industry. Its breeding, cultivation, utilization based on proper germplasm/plant identification, which is difficult using morphological features. For this reason, we applied flow cytometry inter simple sequence repeat polymerase chain reaction (ISSR-PCR) for fast accurate identification. Genome size estimation by was proposed as first-choice method quick accession...

Background The process of apophytism or spreading native species to human-made habitats is one the main elements in creation plant cover anthropogenic areas. Lately, an increase localities with valuable flora has been observed. Apophytes are also members family Orchidaceae, especially from genus Epipactis . aim study was (i) determine and compare phenotypic variation E. helleborine (L.) Crantz plants natural habitats, (ii) genome size growing habitats. results reported this may indicate that...

Gene flow in plant populations is heavily affected by species sexual systems. In order to study the effect of systems on genetic structure, we examined plastid and nuclear DNA 12 dioecious (males females) 18 trioecious (males, females hermaphrodites) Salix myrsinifolia—a boreal shrub with slow range expansion. Populations were located along latitudinal gradients across submarginal marginal parts range. Individuals each sex morph all hexaploid. We identified 10 chloroplast haplotypes scored...

Orchidaceae is one of the largest and most widespread plant families with many species threatened extinction. However, only about 1.5% orchids’ genome sizes have been known so far. The aim this study was to estimate size 15 infraspecific taxon endangered protected orchids growing wild in Poland assess their variability develop additional criterion useful orchid identification characterization. Flow cytometric estimation revealed that investigated possessed intermediate, large, very large...

In the current study, an extensive worldwide herbarium search, including over 1500 sheets of Scolochloa festucacea (Willd.) Lk., was performed to find specimens S. marchica Düvel, Ristow & H. Scholz. The latter is a recently described new species, whose primary distribution found be limited Brandenburg region in Germany and Wolin Island Poland. As result query, not identified any regions other than those previously known. This indicates that it German-Polish endemic with range. Taking...

Several species within the genus Lotus are important forage crops, and many endangered or rare. Despite high genetic diversity of genus, identification is problematic because limited number reliable morphological markers. In search a quick, inexpensive, steady method for identification, genome size cell cycle/endoreduplication intensity 14 accessions belonging to eight were estimated by flow cytometry. ISSR-PCR was also applied find sensitive molecular markers estimation. Genome estimation...

Polyploid specimens are usually characterized by greater exuberance: they reach larger sizes and/or have a number of some organs.

Abstract Flow cytometry estimation of 2C nuclear DNA content the examined Nasturtium species resulted in taxonomic identification N. × sterile eight new localities, microphyllum four localities and officinale one locality western Poland. Scanning electron microscopy proved a few micromorphological traits seeds fruits (size shape cells on fruit septum surface, their anticlinal walls; secondary sculpture outer periclinal walls siliqua valve internal surface) to be importance.

In Poland, isolated serpentine rocks are exclusive habitats of some <em>Asplenium</em> species, reaching here their north or northeastern border range. One them was <em>Asplenium onopteris</em>, a diploid European species native to Mediterranean and Atlantic areas. Since the nineteenth century, Polish out-of-range sites <em>A. onopteris</em> have been quoted in literature without critical verification. Thus, verify occurrence this we analyzed nuclear DNA...

Flow cytometry measurement confirmed the genetic stability of Primula veris L. (cowslip) plants obtained during micropropagation process. The mean 2C DNA content was 0.97 pg/2C and P. can be classified in group very small genomes. Comparative morphological analysis cowslip regenerated vitro derived from seeds carried out three subsequent years under common garden conditions, which limited effects environmental factors. origin specimens significantly influenced most traits examined. Plants...