A5030811915- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- DNA Repair Mechanisms
- Protein Structure and Dynamics
- Skin and Cellular Biology Research
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Enzyme Structure and Function
- Heat shock proteins research
- Mitochondrial Function and Pathology
- RNA modifications and cancer
- Neurobiology and Insect Physiology Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- RNA regulation and disease
- Microbial Metabolites in Food Biotechnology
- Signaling Pathways in Disease
- Microtubule and mitosis dynamics
- Epigenetics and DNA Methylation
- Dermatological and Skeletal Disorders
- Enzyme Production and Characterization
- Genetic Neurodegenerative Diseases
- Hair Growth and Disorders
- S100 Proteins and Annexins
- Biochemical and Structural Characterization
- Genomics and Chromatin Dynamics
Mossakowski Medical Research Institute, Polish Academy of Sciences
2021-2024
Polish Academy of Sciences
2015-2024
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2012-2022
International Institute of Molecular and Cell Biology
2007-2013
Max Planck Institute of Molecular Cell Biology and Genetics
2007-2009
Max Planck Society
2007
Lund University
2007
Beth Israel Deaconess Medical Center
2007
Harvard University
2007
Animal replication-dependent histone pre-mRNAs are processed at the 3′ end by endonucleolytic cleavage that is not followed polyadenylation. The reaction catalyzed CPSF73 and depends on U7 snRNP its integral component, Lsm11. A critical role also played 220-kDa protein FLASH, which interacts with Here we demonstrate N-terminal regions of these two proteins form a platform tightly unique combination polyadenylation factors: symplekin, CstF64, all CPSF subunits, including endonuclease CPSF73....
R.DpnI consists of N-terminal catalytic and C-terminal winged helix domains that are separately specific for the Gm6ATC sequences in Dam-methylated DNA. Here we present a crystal structure with oligoduplexes bound to identify domain residues involved interactions substrate methyl groups. We show these groups target sequence positioned very close each other. further presence two requires deviation from B-DNA conformation avoid steric conflict. The methylation compatible DNA is complementary...
Vimentin intermediate filaments are a significant component of the cytoskeleton in cells mesenchymal origin. In vivo , assemble and disassemble thus participate dynamic processes cell. Post‐translational modifications (PTMs) such as protein phosphorylation regulate multiphasic association vimentin from soluble complexes to insoluble reverse processes. The thiol side chain single cysteine at position 328 (Cys328) is direct target oxidative inside cells. Here, we used atomic force microscopy,...
Superoxide dismutase 1 (Sod1) is a major superoxide-scavenging enzyme in the eukaryotic cell, and localized cytosol intermembrane space of mitochondria. Sod1 requires its specific chaperone Ccs1 disulfide bond formation order to be retained space. Our study identified pool that present reduced state mitochondria lack Ccs1. We created yeast mutants with mutations highly conserved amino acid residues corresponding human cause amyotrophic lateral sclerosis, found some mutant proteins were...
Restriction endonuclease MvaI recognizes the sequence CC/WGG (W stands for A or T, ‘/’ designates cleavage site) and generates products with single nucleotide 5′-overhangs. The enzyme has been noted its tolerance towards DNA modifications. Here, we report a biochemical characterization crystal structures of in an apo-form complex target at 1.5 Å resolution. Our results show that is monomer pseudosymmetric asymmetrically. consists two lobes. catalytic lobe anchors active site residues Glu36,...
Mutations that impair expression or function of the components phagocyte NADPH oxidase complex cause chronic granulomatous disease (CGD), which is associated with life-threatening infections and dysregulated inflammation. In five CGD patients from four consanguineous families two different ethnic backgrounds, we found similar genomic homozygous deletions 1,380 bp comprising exon 5 NCF2, could be traced to Alu-mediated recombination events. cDNA sequencing showed in-frame phase zero 5,...
RACK1 is a member of the WD repeat family proteins and involved in multiple fundamental cellular processes. An intriguing feature its ability to interact with at least 80 different protein partners. Thus, structural features enabling such interactomic flexibility are great interest. Several previous studies crystal structures orthologs described detailed architecture confirmed predictions that adopts seven-bladed β-propeller fold. However, this did not explain bind We performed...
Peptidoglycan, a gigadalton polymer, functions as the scaffold for bacterial cell walls and provides integrity. Peptidoglycan is remodelled by large diverse group of peptidoglycan hydrolases, which control growth division. Over years, many studies have focused on these enzymes, but knowledge their action within mesh from molecular basis scarce. Here, we provide structural insights into interaction between short fragments entire sacculus with two evolutionarily related peptidases M23 family,...
Nuclear receptors (NRs) are a family of ligand-dependent transcription factors activated by lipophilic compounds. NRs share common structure comprising three domains: variable N-terminal domain (NTD), highly conserved globular DNA-binding and ligand-binding domain. There numerous papers describing the molecular details latter two domains. However, very little is known about structure-function relationship NTD, especially as an intrinsically disordered fragment that may influence properties...
Nucleobindin-2 (Nucb2) is a protein that has been suggested to play roles in variety of biological processes. Nucb2 contains two Ca2+/Mg2+-binding EF-hand domains separated by an acidic amino acid residue-rich region and leucine zipper. All these are located within the C-terminal half protein. At N-terminal half, also possesses putative Zn2+-binding motif. In our recent studies, we observed underwent Ca2+-dependent compaction formed mosaic-like structure consisting intertwined disordered...
Lipid anchors are common post-translational modifications for proteins engaged in signaling and vesicular transport eukaryotic cells. Rab geranylgeranylated at their C-termini, a modification which is important stable binding to lipid bilayers. The escort protein (REP) an accessory of the geranylgeranyl transferase (RGT) complex it obligatory prenylation. While REP-Rab interactions have been studied by biochemical, structural, genetic methods animals yeast, data on plant RGT still limited....
Two major lipophilic hormones, 20-hydroxyecdysone (20E) and juvenile hormone (JH), govern insect development growth. While the mode of action 20E is well understood, some understanding JH-dependent signalling has been attained only in past few years, crosstalk two hormonal pathways remains unknown. proteins, calponin-like Chd64 immunophilin FKBP39 have recently found to play pivotal roles formation dynamic, multiprotein complex that cross-links these pathways. However, molecular mechanism...
Mastitis is an inflammatory disease of the mammary gland commonly brought about by bacterial pathogens that gain physical access to glandular epithelium through teat canal. In bovines, common mastitis-causing agents are environmental or pathogenic species, including staphylococci, streptococci, enterococci, and Gram-negative bacteria such as Escherichia coli. Current therapeutic strategies for bovine mastitis typically involve administration antibiotic formulations within infected udder,...
Abstract CK2 is a member of the CMGC group eukaryotic protein kinases and cancer drug target. It can be efficiently inhibited by halogenated benzotriazoles benzimidazoles. Depending on scaffold, substitution pattern, pH, these compounds are either neutral or anionic. Their binding poses dictated hydrophobic effect (desolvation) tug war between salt bridge/hydrogen bond (to K68) halogen bonding E114 V116 backbone oxygens). Here, we test idea that might controllable pH for ligands with...