The inner ear of the Danio rerio fish contains biominerals called otoliths that are composed calcium carbonate crystals and a protein organic matrix. It has been previously suggested Starmaker (Stm), which is an intrinsically disordered protein, acts as component controls size, shape, polymorph crystals. In this study, in vitro crystallization system was established to examine role Stm extensively phosphorylated (StmP) formation SEM X-ray diffraction analyses indicated dimensions calcite...

Otolin‐1 is a collagen‐like protein expressed in the inner ear of vertebrates. It provides an organic scaffold for otoliths fish and otoconia land In this study, expression purification procedure C1q‐like domain otolin‐1 from human zebrafish was developed. The structure stability proteins were investigated. results sedimentation velocity analytical ultracentrifugation small‐angle X‐ray scattering indicated that forms stable trimers solution presence calcium ions. also observed ions...

Starmaker (Stm) is an intrinsically disordered protein (IDP) involved in otolith biomineralization Danio rerio. Stm controls calcium carbonate crystal formation vivo and vitro. Phosphorylation of affects its properties. This study examined the effects ions phosphorylation on structure Stm. We have shown that CK2 kinase phosphorylates 25 or 26 residues Furthermore, we demonstrated Stm's affinity for binding dependent state. Phosphorylated (StmP) has estimated 30 ± 1 sites per molecule with a...

Otoliths are one of the biominerals whose formation is highly controlled by proteins. The first protein discovered to be involved in otolith biomineralization zebrafish was starmaker (Stm). Previously, Stm shown responsible for preferential aragonite, a polymorph calcium carbonate, otoliths. In this work, proteomic analysis adult otoliths performed. only phosphorylated found our studies. Besides previously studied proteins, we several dozens unknown proteins that reveal likely mechanism...

Otoliths and otoconia are calcium carbonate biomineral structures that form in the inner ear of fish humans, respectively. The formation these is tightly linked to an organic matrix framework with otolin-1, a short collagen-like protein from C1q family as one its major constituents.In this study, we examined activity recombinant otolin-1 originating Danio rerio Homo sapiens on bioinspired mineralization slow-diffusion method performed crystals characterization scanning electron microscopy,...

We present here a new assay that is based on the idea of molecular beacon. This makes it possible to investigate two proteins interacting with DNA at binding sites are close each other. The effectiveness test depends exclusive three fragments in presence proteins, and monitoring process upon observing quenching independent fluorescence donors. As model we used components heterodimeric ecdysteroid receptor ultraspiracle (Usp) ecdysone (EcR) from Drosophila melanogaster response element...

Ecdysteroids coordinate molting and metamorphosis in insects via a heterodimer of two nuclear receptors, the ecdysone receptor (EcR) ultraspiracle (Usp) protein. Here we show how DNA-recognition alpha-helix T box region EcR DNA-binding domain (EcRDBD) contribute to specific interaction with natural response element stabilization EcRDBD molecule. The data indicate remarkable mutational tolerance respect function EcRDBD. This is particularly manifested heterocomplexes formed between mutants...

The binding properties of low- and high-adhesive forms FimH adhesins from Salmonella enterica serovars Enteritidis Typhimurium ( S. Typhimurium) were studied using chimeric proteins containing an additional peptide that represents N-terminal extension the FimF protein. This modification, by taking advantage a donor strand exchange mechanism, closes hydrophobic groove in fimbrial domain adhesin. Such self-complemented (scFimH) did not form aggregates more stable (resistant to proteolytic...

Muscle fructose 1,6-bisphosphatase (FBP2), besides being a regulatory enzyme of glyconeogenesis also protects mitochondria against calcium stress and plays key role in regulation the cell cycle, promoting cardiomyocytes survival. However, cancer cells, FBP2 acts as an anti-oncogenic/anti-proliferative protein. Here, we show that physiological function depends both on its level expression well oligomerization state. Animal fructose-1,6-bisphosphatases are thought to tetramers. We present...

Human transthyretin (TTR) is a tetrameric protein transporting thyroid hormones and retinol. TTR neuroprotective factor sensor of oxidative stress which stability diminished due to mutations aging, leading amyloid deposition. Adverse environmental conditions, such as redox metal ion imbalances, induce destabilization the structure. We have previously shown that was disturbed by Ca2+ other factors, including DTT, led formation an intrinsic fluorophore(s) emitting blue light, termed deep...

The inner ear of the rainbow trout contains biominerals called otoliths that are composed calcium carbonate crystals and a protein organic matrix. matrix macromolecule-64 (OMM-64) is an intrinsically disordered associated with high-molecular-weight aggregates. In this study, through series bioinspired mineralization experiments, we have determined OMM-64 affects size shape CaCO3 stiffens crystals' surfaces. serves as template for precipitating inorganic phase, which causes greater...

Drosophila hormone receptor 38 (dHR38), an ortholog of the vertebrate NR4A subclass nuclear receptors, responds to ecdysteroids, which mediate developmental transitions during life cycle. However, this response is independent ecdysteroid receptor, and it does not involve binding ecdysteroids dHR38. It has been suggested that may indirectly activate dHR38, perhaps by recruiting specific proteins. There have recent reports pointing out decisive role N-terminal domains (NTDs) in protein-protein...

Juvenile hormone-binding protein (JHBP) isolated from Galleria mellonella haemolymph has been crystallized using the hanging-drop method in two polymorphic forms. The best diffracting crystals (2.7 A) are trigonal, space group P3(1)21 (or P3(2)21), with unit-cell parameters a = 110.4, c 93.9 A. X-ray diffraction data have collected for native synchrotron radiation and cryogenic conditions (100 K).

Abstract Ecdysteroids coordinate essential biological processes in Drosophila through a complex of two nuclear receptors, the ecdysteroid receptor (EcR) and ultraspiracle protein (Usp). Biochemical experiments have shown that, contrast to Usp, EcR molecule is characterized by high intramolecular plasticity. To investigate whether this plasticity sufficient form complexes with receptors other than we studied interaction DHR38 receptor. Previous vitro suggested that can Usp thus disrupt...

Dentin matrix protein 1 (DMP1) is an acidic, extracellular essential for biomineralization of calcium phosphate, in bone and dentin. It proteolytically processed into two fragments, 44K 56K. Recently, the presence DMP1 was noticed inner ear, specifically otoconia, which are carbonate biominerals involved sensing balance. In this study, solution structure activity otoconial 56K fragments toward were investigated. The results analytical ultracentrifugation, circular dichroism, gel filtration...

Fish otoliths are calcium carbonate biominerals found in the inner ear commonly used for tracking fish biochronologies and as a model system biomineralization. The process of otolith formation is biologically controlled by numerous biomacromolecules which not only affect crystal size, shape, mechanical properties, but also selection polymorph (e.g., aragonite, vaterite). proteinaceous control over occurs many other species corals, mollusks, echinoderms) exact mechanism protein interactions...

Mineralization in living organisms is highly regulated by the synergistic action of many macromolecules, where distinctive proteins play role direct modulators crystal growth. The number methods for vitro biomineralization studies presence limited due to influence environment on their functionality. We focus a counter-diffusion system calcium carbonate that based diffusion salt solutions through gel-matrix supplemented with protein interest. This article discusses background system, physical...

The nonstandard molecular beacon described in this article consists of 2 fragments, each built a short single-stranded oligonucleotide sequence and double-stranded sequence.One these hybridization probes, labeled with fluorescence donor (fluorescein), is solid phase immobilized.The second nonimmobilized probe quencher (dabcyl).Annealing both probes via sequences was possible only the presence specific protein molecule that recognized response element initially separated between immobilized...

The hemolymph juvenile hormone binding protein (JHBP) from Galleria mellonella contains two disulphide bridges/molecule and no free Cys residues. An alignment of primary structures other Lepidopteran JHBPs indicates that residues, equivalent to Cys10,17,151,195 in G. JHBP, maybe involved -S-S- bridge formation.