A5031481144- Enzyme Structure and Function
- Crystallography and molecular interactions
- Protein Structure and Dynamics
- Biochemical and Molecular Research
- Crystal structures of chemical compounds
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Synthesis and Characterization of Heterocyclic Compounds
- Acute Lymphoblastic Leukemia research
- Enzyme Production and Characterization
- DNA and Nucleic Acid Chemistry
- HIV/AIDS drug development and treatment
- Carbohydrate Chemistry and Synthesis
- Legume Nitrogen Fixing Symbiosis
- RNA and protein synthesis mechanisms
- HIV Research and Treatment
- Folate and B Vitamins Research
- Botanical Research and Chemistry
- Computational Drug Discovery Methods
- Cancer, Hypoxia, and Metabolism
- Structural and Chemical Analysis of Organic and Inorganic Compounds
- Biochemical and Structural Characterization
- Photosynthetic Processes and Mechanisms
- Synthesis and Reactions of Organic Compounds
- Analytical Chemistry and Chromatography
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2016-2025
Adam Mickiewicz University in Poznań
2016-2025
Poznań University of Technology
2019
Miros (Norway)
2019
University of Rochester
2019
National Cancer Institute
1991-2017
University of Virginia
2017
Polish Academy of Sciences
1996-2015
Koszalin University of Technology
2010-2015
Argonne National Laboratory
2014
The rational design of drugs that can inhibit the action viral proteases depends on obtaining accurate structures these enzymes. crystal structure chemically synthesized HIV-1 protease has been determined at 2.8 angstrom resolution (R factor 0.184) with use a model based Rous sarcoma virus structure. In this enzymatically active protein, cysteines were replaced by alpha-amino-n-butyric acid, nongenetically coded amino acid. This structure, in which all 99 acids located, differs several...
The plant circadian clock is proposed to be a network of several interconnected feedback loops, and loss any component leads changes in oscillator speed. We previously reported that Arabidopsis thaliana EARLY FLOWERING4 (ELF4) required sustain this the elf4 mutant arrhythmic. This phenotype shared with both elf3 lux. Here, we show overexpression either ELF3 or LUX ARRHYTHMO (LUX) complements phenotype. Furthermore, ELF4 causes form foci nucleus. used expression data direct mathematical...
The crystal structure of Escherichia coli asparaginase II (EC 3.5.1.1), a drug (Elspar) used for the treatment acute lymphoblastic leukemia, has been determined at 2.3 A resolution by using data from single heavy atom derivative in combination with molecular replacement. atomic model was refined to an R factor 0.143. This enzyme, active as homotetramer 222 symmetry, belongs class alpha/beta proteins. Each subunit two domains unique topological features. On basis present structural evidence...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructure at 2.5-.ANG. resolution of chemically synthesized Human Immunodeficiency Virus Type 1 protease complexed with a hydroxyethylene-based inhibitorMariusz Jaskolski, Alfredo G. Tomasselli, Tomi K. Sawyer, Douglas Staples, Robert L. Heinrikson, Jens Schneider, Stephen B. H. Kent, and Alexander WlodawerCite this: Biochemistry 1991, 30, 6, 1600–1609Publication Date (Print):February 12, 1991Publication History Published online1 May 2002Published...
Molecular tapes mediated via strong O–H⋯N hydrogen bonds and weak C–I⋯O interactions are present in complexes of 4-nitrobenzoic acid·4-iodopyridine 3,5-dinitrobenzoic acid·4-iodopyridine. Crystal structure 4-nitrobenzamide·4-iodobenzamide has amide dimer tape iodo⋯nitro interaction orthogonal directions. There is good structural insulation the bonding halogen domains these crystal structures. However, less polarizable bromine chlorine atoms show cross-pairing that predictable. These...
The human immunodeficiency virus (HIV-1) encodes a protease that is essential for viral replication and member of the aspartic family. recently determined three-dimensional structure related from Rous sarcoma has been used to model smaller HIV-1 dimer. active site analyzed by comparison protease, rhizopuspepsin, complexed with peptide inhibitor. predicted interact seven residues protein substrate. This information can be design inhibitors possible antiviral drugs.
Cysteine proteases (CPs) are responsible for many biochemical processes occurring in living organisms and they have been implicated the development progression of several diseases that involve abnormal protein turnover. The activity CPs is regulated among others by their specific inhibitors: cystatins. main aim this review to discuss structure-activity relationships cysteine cystatins, as well some synthetic inhibitors structurally based on binding fragments
Enzymes capable of converting L-asparagine to L-aspartate can be classified as bacterial-type or plant-type L-asparaginases. Bacterial-type L-asparaginases are further divided into subtypes I and II, defined by their intra-/extra-cellular localization, substrate affinity, oligomeric form. Plant-type evolutionarily structurally distinct from the enzymes. They function potassium-dependent -independent Ntn-hydrolases, similar well characterized aspartylglucosaminidases with (alphabeta)2...
Cystatin C and the prion protein have been shown to form dimers via three-dimensional domain swapping, this process has also hypothesized be involved in amyloidogenesis. Production of oligomers other amyloidogenic proteins reported precede fibril formation, suggesting as intermediates fibrillogenesis. A variant cystatin C, with a Leu68-->Gln substitution, is highly amyloidogenic, carriers mutation suffer from massive cerebral amyloidosis leading brain hemorrhage death early adulthood. This...
Human cystatin C (HCC) is a family 2 inhibitor of papain-like (C1) and legumain-related (C13) cysteine proteases. In pathophysiological processes, the nature which not understood, HCC codeposited in amyloid plaques Alzheimer's disease or Down's syndrome. The amyloidogenic properties are greatly increased naturally occurring L68Q variant, resulting fatal cerebral angiopathy early adult life. all crystal structures studied to date, protein has been found form 3D domain-swapped dimers, created...
Whereas the vast majority of more than 85 000 crystal structures macromolecules currently deposited in Protein Data Bank are high quality, some suffer from a variety imperfections. Although this fact has been pointed out past, it is still worth periodic updates so that metadata obtained by global analysis available structures, as well utilization individual for tasks such drug design, should be based on only most reliable data. Here, selected abnormal have analysed Bayesian reasoning...
L-Asparaginases, divided into three structural Classes, catalyze the hydrolysis of L-asparagine to L-aspartic acid and ammonia. The members Class 3, ReAIV ReAV, encoded in genome nitrogen fixing Rhizobium etli , have same fold, active site, quaternary structure, despite low sequence identity. In present work we examined biochemical consequences this difference. is almost twice as efficient ReAV asparagine at 37°C, with kinetic K M k cat parameters (measured optimal buffering agent) 1.5 mM,...
Asparaginases catalyze the hydrolysis of asparagine to aspartic acid and ammonia. Enzymes with asparaginase activity play an important role both in metabolism all living organisms as well pharmacology. The main goal this paper is attempt a classification known enzymes activity, based on their amino sequences. Some possible phylogenetic consequences are also discussed using dendrograms structural information derived from crystallographic studies.
Amyloidogenic proteins like cystatin C and prion have been shown to form dimers by exchange of subdomains the monomeric proteins. This process, called "three-dimensional domain swapping," has also suggested play a part in generation amyloid fibrils. One variant C, L68Q is highly amyloidogenic, persons carrying corresponding gene suffer from massive cerebral amyloidosis leading brain hemorrhage death early adult life. The present work describes production two variants wild type with disulfide...