A5078402907- Acute Lymphoblastic Leukemia research
- Biochemical and Molecular Research
- Legume Nitrogen Fixing Symbiosis
- Porphyrin Metabolism and Disorders
- Folate and B Vitamins Research
- Chronic Lymphocytic Leukemia Research
- Enzyme Structure and Function
- Proteins in Food Systems
- Protein Interaction Studies and Fluorescence Analysis
- Neonatal Health and Biochemistry
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2022-2025
Jagiellonian University
2022
L-Asparaginases, divided into three structural Classes, catalyze the hydrolysis of L-asparagine to L-aspartic acid and ammonia. The members Class 3, ReAIV ReAV, encoded in genome nitrogen fixing Rhizobium etli , have same fold, active site, quaternary structure, despite low sequence identity. In present work we examined biochemical consequences this difference. is almost twice as efficient ReAV asparagine at 37°C, with kinetic K M k cat parameters (measured optimal buffering agent) 1.5 mM,...
Rhizobium etli is a nitrogen-fixing bacterium that encodes two l-asparaginases. The structure of the inducible R. asparaginase ReAV has been recently determined to reveal protein with no similarity known enzymes l-asparaginase activity, but showing curious resemblance glutaminases and β-lactamases. uniqueness sequence 3D make enzyme an interesting candidate as potential replacement for immunogenic bacterial-type asparaginases are currently in use treatment acute lymphoblastic leukemia....
The genome of Rhizobium etli , a nitrogen-fixing bacterial symbiont legume plants, encodes two L-asparaginases, ReAIV and ReAV, that have no similarity to the well characterized enzymes class 1 (bacterial type) 2 (plant type). It has been hypothesized ReAV might belong same structural 3 despite their low level sequence identity. When crystal structure inducible thermolabile protein was solved, this hypothesis gained stronger footing because key residues are also present in constitutive...
ReAV, the inducible Class-3 L-asparaginase from nitrogen-fixing symbiotic bacterium Rhizobium etli , is an interesting candidate for optimizing its enzymatic potential antileukemic applications. Since it has no structural similarity to known enzymes with this activity, may offer completely new ways of approach. Also, as unrelated protein, would evade immunological response elicited by other asparaginases. The crystal structure ReAV revealed a uniquely assembled protein homodimer highly...
The homodimeric β-lactoglobulin belongs to the lipocalin family of proteins that transport a wide range hydrophobic molecules and can be modified by mutagenesis develop specificity for novel groups ligands. In this work, new lactoglobulin variants, FAF (I56F/L39A/M107F) FAW (I56F/L39A/M107W), were produced their interactions with tricyclic drug desipramine (DSM) studied using X-ray crystallography, calorimetry (ITC) circular dichroism (CD). ITC CD data showed micromolar affinity mutants DSM...
L-asparaginases are used in the treatment of acute lymphoblastic leukemia. The aim this work was to compare antiproliferative potential and proapoptotic properties novel from different structural classes, viz. EcAIII KpAIII (class 2), as well ReAIV ReAV 3). EcAII 1) enzyme served a reference. effects were tested using four human leukemia cell models: MOLT-4, RAJI, THP-1, HL-60. assay with MOLT-4 line indicated inhibitory all L-asparaginases. results THP-1 models showed similar effect...