A5080126091- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Vasculitis and related conditions
- Adipose Tissue and Metabolism
- Heme Oxygenase-1 and Carbon Monoxide
- Sulfur Compounds in Biology
- Regulation of Appetite and Obesity
- Adipokines, Inflammation, and Metabolic Diseases
- Nutrition and Health in Aging
- Atherosclerosis and Cardiovascular Diseases
- Biomarkers in Disease Mechanisms
- COVID-19 Clinical Research Studies
- Exercise and Physiological Responses
- Gastroesophageal reflux and treatments
- Childhood Cancer Survivors' Quality of Life
- Microscopic Colitis
- Extracellular vesicles in disease
- Inflammasome and immune disorders
- Asthma and respiratory diseases
- Gut microbiota and health
- Inflammatory Biomarkers in Disease Prognosis
- Alkaline Phosphatase Research Studies
- Gastrointestinal motility and disorders
- Mechanical Circulatory Support Devices
- Neuropeptides and Animal Physiology
- Diet and metabolism studies
Jagiellonian University
2015-2024
Weatherford College
2009
Uniwersytecki Szpital Dziecięcy
2008
Tumour-derived microvesicles (TMVs) are important players in tumour progression, modulating biological activity of immune cells e.g. lymphocytes, monocytes and macrophages. This phenomenon is particularly interesting the progression colon cancer, as macrophages this type relevant for recovery processes. In present study, role cancer cell-derived monocyte differentiation profile (polarization) was investigated. Monocyte-derived (MDM) were differentiated vitro presence TMVs obtained from...
Inflammatory bowel diseases (IBDs) are a heterogeneous group of disorders exhibited by two major phenotypic forms: Crohn‘s disease and ulcerative colitis. Although the aetiology IBD is unknown, several factors coming from adipose tissue skeletal muscles, such as cytokines, adipokines myokines, were suggested in pathogenesis colitis; however, it has not been extensively studied whether voluntary exercise can ameliorate that disorder. We explored effect moderate (i.e., wheel running) on...
Endogenous gaseous mediators, such as nitric oxide, hydrogen sulfide or carbon monoxide (CO) are known to exert anti-inflammatory and anti-oxidative activity due modulation of various molecular pahtways. Therefore, we aimed investigate if CO released from tricarbonyldichlororuthenium (II) dimer (CORM-2) prevents gastric mucosa against ischemia/reperfusion (I/R)-induced injury in male Wistar rats. Animals were pretreated i.g. With vehicle (DMSO saline, 1:10), CORM-2 (1, 5 10 mg/kg) zinc...
Metal-based carbon monoxide (CO)-releasing molecules have been shown to exert anti-inflammatory and anti-oxidative properties maintaining gastric mucosal integrity. We are interested in further development of metal-free CO-based therapeutics for oral administration. Thus, we examine the protective effect representative CO prodrug, BW-CO-111, rat models damage induced by necrotic ethanol or aspirin, a non-steroidal drug. Treatment effectiveness was assessed measuring microscopic/macroscopic...
The SARS-CoV-2 infection [coronavirus disease 2019 (COVID-19)] is associated with severe lymphopenia and impaired immune response, including expansion of myeloid cells regulatory functions, e.g., so-called low-density neutrophils, containing granulocytic myeloid-derived suppressor (LDNs/PMN-MDSCs). These have been described in both infections cancer are known for their immunosuppressive activity. In the case COVID-19, long-term complications frequently observed (long-COVID). this context, we...
Hydrogen sulfide (H2S) plays an important role in human physiology, exerting vasodilatory, neuromodulatory and anti-inflammatory effects. H2S has been implicated the mechanism of gastrointestinal integrity but whether this gaseous mediator can affect hemorrhagic lesions induced by stress little elucidated. We studied effect precursor L-cysteine, H2S-donor NaHS, synthesizing enzyme (CSE) activity inhibitor- D,L-propargylglycine (PAG) gastric production CSE/CBS/3-MST water immersion restraint...
The physiological gaseous molecule, carbon monoxide (CO) becomes a subject of extensive investigation due to its vasoactive activity throughout the body but role in gastroprotection has been little investigated. We determined mechanism CO released from donor tricarbonyldichlororuthenium (II) dimer (CORM-2) protection gastric mucosa against 75% ethanol-induced injury. Rats were pretreated with CORM-2 30 min prior ethanol or without 1) non-selective (indomethacin) selective cyclooxygenase...
Curcumin, a pleiotropic substance used for centuries in traditional medicine, exhibits antioxidant, anti-inflammatory and antiproliferative efficacy against various tumours, but the role of curcumin gastroprotection is little studied. We determined effect gastric haemorrhagic lesions induced by 75% ethanol alterations blood flow (GBF) rats with cyclooxygenase-1 (COX-1) COX-2 activity inhibited indomethacin, SC-560 or rofecoxib, NO-synthase activity, capsaicin denervation blockade TRPV1...
The objective of our study was to evaluate the T-helper (Th) and regulatory T (Treg) cell profile in ANCA-positive granulomatosis with polyangiitis (GPA) its relation disease activity. In a prospective study, we studied two groups GPA patients: (i) flare (active-GPA, BVAS>6, n = 19), (ii) sustained remission (≥ 1-year prior enrollment, inactive-GPA, BVAS 0, 18). 24 age-sex matched healthy subjects served as controls. Active-GPA patients were followed for 6 months reevaluated during (early...
Carbon monoxide (CO) produced by heme oxygenase (HO)-1 and HO-2 or released from the CO-donor, tricarbonyldichlororuthenium (II) dimer (CORM-2) causes vasodilation, with unknown efficacy against stress-induced gastric lesions. We studied whether pretreatment CORM-2 (0.1–10 mg/kg oral gavage (i.g.)), RuCl3 (1 i.g.), zinc protoporphyrin IX (ZnPP) (10 intraperitoneally (i.p.)), hemin (1–10 i.g.) combined NG-nitro-l-arginine (l-NNA, 20 i.p.), 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ, 10...
Alendronate is an inhibitor of osteoclast-mediated bone resorption, but its clinical utility limited due to gastrointestinal complications including bleeding erosions. We studied whether potent vasodilators hydrogen sulfide (H2S) and carbon monoxide (CO) can protect against alendronate-induced gastric lesions in rats exposed mild stress. Three series (A, B, C) Wistar received alendronate (150–700 mg/kg i.g., A) with or without NaHS (5 mg/kg), H2S donor CORM-2 mg/kg) releasing CO administered...
Abstract Human rhinoviruses (HRV) are frequent cause of asthma exacerbations, however the influence airway inflammation on severity viral infection is poorly understood. Here, we investigated how cytokine-induced remodeling epithelium modulates antiviral response. We analyzed gene expression response in vitro differentiated bronchial exposed to cytokines and next infected with HRV16. IL-13-induced mucous cell metaplasia (MCM) was associated impaired ciliogenesis induction genes, resulting...
Summary Neutrophil is a key cell in pathophysiology of granulomatosis with polyangiitis. Recently, neutrophil extracellular traps were described this disease. Mitochondrial DNA also released during formation. We measured circulating cell-free mitochondrial and genomic serum patients Subjects the disease (14 active 11 remission stage) 10 healthy controls enrolled. Quantitative real-time polymerase chain reaction (PCR) was used to measure 79 base pairs (bp) 230 bp mtDNA fragments. Alu repeats...
Approximately 20% of children and adolescents in Europe are overweight. Survivors pediatric acute lymphoblastic leukemia (ALL) at increased risk overweight obesity. The purpose this study was to assess leptin soluble receptor levels, as well polymorphisms selected genes survivors ALL, the influence chemo- radiotherapy on development context regulation. Eighty two patients (55% males), median age 13.2 years (m: 4.8 years; M: 26.2 years) were included study. ALL therapy conducted according...
In vasculitis disorders, inflammation affects blood vessels. Granulomatosis with polyangiitis (GPA) is a chronic systemic distinguished by the presence of anti-proteinase-3 autoantibodies (anti-PR3). this study we analyzed molecular signature human umbilical endothelial cells (HUVECs) in response to neutrophil-derived extracellular vesicles (EVs). EVs were obtained from anti-PR3-activated neutrophils, purified and characterized flow cytometry, nanoparticle tracking miRNA screening. HUVECs...
Inflammatory bowel diseases (IBD) are commonly considered as Crohn's disease and ulcerative colitis, but the possibility that alterations in gut microbiota oxidative stress may affect course of experimental colitis obese physically exercising mice treated with intestinal alkaline phosphatase (IAP) has been little elucidated. Mice fed a high-fat-diet (HFD) or normal diet (ND) for 14 weeks were randomly assigned to exercise on spinning wheels (SW) 7 IAP followed by intrarectal administration...
Intestinal alkaline phosphatase (IAP) is an enzyme that plays a protective role in the gut. This study investigated effect of IAP treatment on experimental colitis mice subjected to forced exercise high-fat diet. C57BL/6 with TNBS were fed diet and treadmill or without treatment. Disease activity, oxidative stress, inflammatory cytokines, gut microbiota assessed. Forced exacerbated obese mice, as evidenced by increased disease activity index (DAI), stress markers, proinflammatory adipokines...
Inflammatory bowel diseases are a heterogeneous group of disorders represented by two major phenotypic forms, Crohn’s disease and ulcerative colitis. Cross talk between adipokines myokines, as well changes in intestinal microcirculation, was proposed pathogenesis these disorders. C57BL/6 male mice were fed ad libitum for 12 weeks standard (SD) or high-fat diet (HFD). After the adaptation period, groups animals SD HFD subjected to 6 forced treadmill exercise experimental colitis induced both...