A5041514975- Asthma and respiratory diseases
- Respiratory and Cough-Related Research
- Systemic Lupus Erythematosus Research
- Neonatal Respiratory Health Research
- IL-33, ST2, and ILC Pathways
- T-cell and B-cell Immunology
- Respiratory viral infections research
- Inhalation and Respiratory Drug Delivery
- Vasculitis and related conditions
- Urticaria and Related Conditions
- Eosinophilic Disorders and Syndromes
- Atherosclerosis and Cardiovascular Diseases
- Pediatric health and respiratory diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Drug-Induced Adverse Reactions
- MicroRNA in disease regulation
- Syphilis Diagnosis and Treatment
- Reproductive tract infections research
- Platelet Disorders and Treatments
- SARS-CoV-2 and COVID-19 Research
- Mast cells and histamine
- Inflammasome and immune disorders
- Monoclonal and Polyclonal Antibodies Research
- Heparin-Induced Thrombocytopenia and Thrombosis
- Antiplatelet Therapy and Cardiovascular Diseases
Jagiellonian University
2016-2025
Stanford University
2020
University of Wisconsin–Madison
2007
BackgroundHuman rhinoviruses (HRVs) are the most prevalent human pathogens, and consist of 101 serotypes that classified into groups A B according to sequence variations. HRV infections cause a wide spectrum clinical outcomes ranging from asymptomatic infection severe lower respiratory symptoms. Defining role specific strains in various illnesses has been difficult because traditional serology, which requires viral culture neutralization tests using serotype-specific antisera, is insensitive...
We used an in vitro model of differentiated tracheobronchial epithelium to analyze the susceptibility different cell types infection with rhinoviruses (RVs). Primary cells from control subjects were cultured air-liquid interface form epithelia. Suprabasal and basal fractions separated after trypsin digestion, suspensions infected serotypes RV16 RV1A. These analyzed for expression viral capsid protein VP2 (flow cytometry), replication (real-time PCR), cytokeratin-14, intercellular adhesion...
Objective. Churg–Strauss syndrome (CSS) is a rare systemic vasculitis associated with eosinophilia and asthma. We assessed the local immune response in airways of CSS patients different activity disease.
Abstract Rhinoviruses and allergens, such as house dust mite are major agents responsible for asthma exacerbations. The influence of pre-existing airway inflammation on the infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is largely unknown. We analyse mechanisms response to viral in experimental vivo rhinovirus healthy controls patients asthma, vitro experiments mite, SARS-CoV-2 human primary epithelium. Here, we show that leads an excessive RIG-I inflammasome...
Impaired function of regulatory T-cells (Treg) leads to a failure in immune tolerance and triggers autoimmunity. We analyzed whether the deficiency Treg systemic lupus erythematosus (SLE) is accompanied by an increase effector T-cell responses. studied frequencies IL-17A (Th17) IFNg (Th1) producing CD4(+) flow cytometric detection intracellular cytokines PMA/ionomycin stimulated blood lymphocytes from seven patients with active SLE, eight SLE remission, 11 healthy controls. Circulating were...
Cold viruses have generally been considered fairly innocuous until the appearance of severe acute respiratory coronavirus 2 (SARS-CoV-2) in 2019, which caused disease 2019 (COVID-19) global pandemic. Two previous foreshadowed that a could potentially devastating consequences 2002 [severe (SARS-CoV)] and 2012 [Middle East syndrome (MERS-CoV)]. The question arises is why these are so different from relatively harmless cold viruses. On basis an analysis current literature using bioinformatic...
15-oxo-eicosatetraenoic acid (15-oxo-ETE), is a product of arachidonic (AA) metabolism in the 15-lipoxygenase-1 (15-LOX-1) pathway. 15-oxo-ETE was overproduced nasal polyps patients with nonsteroidal anti-inflammatory drug-exacerbated respiratory disease (N-ERD). In this study we investigated systemic biosynthesis and leukotriene E4 (LTE4) assessed their diagnostic value to identify N-ERD. The included 64 N-ERD, 59 asthmatics who tolerated aspirin well (ATA), 51 healthy controls. A thorough...
Human rhinoviruses (RVs) are a major cause of exacerbations in asthma and other chronic airway diseases. A characteristic feature asthmatic epithelium is goblet cell metaplasia mucus hypersecretion. Bronchial also an important source lipid mediators, including pro- antiinflammatory eicosanoids. By using air-liquid interface cultures from patients with nonasthmatic control subjects, we compared RV16 replication-induced changes mRNA expression candidate genes eicosanoid production the or...
Abstract Rhinovirus (RV) infections are associated with asthma exacerbations. MicroRNA‐146a and microRNA‐146b (miR‐146a/b) anti‐inflammatory miRNAs that suppress signaling through the nuclear factor kappa B (NF‐κB) pathway inhibit pro‐inflammatory chemokine production in primary human bronchial epithelial cells (HBECs). In current study, we aimed to explore whether miR‐146a/b could regulate cellular responses RVs HBECs airways during RV‐induced exacerbation. We demonstrated expression of...
Hypoxic conditions induce the expression of hypoxia-inducible factors (HIFs) that allow cells to adapt changing and alter a number genes including cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is low abundance mRNA in airway epithelial even during normoxic conditions, but hypoxia its decreases further. In current studies, we examined kinetics hypoxia-induced changes protein levels two human cell lines, Calu-3 16HBE14o-, normal primary bronchial cells. Our goal was examine...
The objective of our study was to evaluate the T-helper (Th) and regulatory T (Treg) cell profile in ANCA-positive granulomatosis with polyangiitis (GPA) its relation disease activity. In a prospective study, we studied two groups GPA patients: (i) flare (active-GPA, BVAS>6, n = 19), (ii) sustained remission (≥ 1-year prior enrollment, inactive-GPA, BVAS 0, 18). 24 age-sex matched healthy subjects served as controls. Active-GPA patients were followed for 6 months reevaluated during (early...
Background Asthma is associated with a prothrombotic state. Plasma factor VIIa–antithrombin complex (FVIIa-AT) concentrations indirectly reflect the interaction of tissue (TF) FVII. Since TF key initiator coagulation in vivo, we hypothesized that FVIIa-AT are higher asthma. Methods In 159 clinically stable adult asthma patients and 62 controls, determined plasma analyzed their relation to circulating inflammatory markers together total potential for fibrinolysis (clot lysis time, CLT)...
ABSTRACT Background Asthma exacerbations are frequently triggered by human rhinoviruses (RVs). Among other pro‐inflammatory responses, RV infection of airway epithelium promotes the activation inflammasome pathway, role which in asthma and disease progression is still poorly understood. Methods Bronchial brushing or biopsy specimens were collected from patients control subjects. Functional experiments performed cultured bronchial epithelial cells (HBECs) using RV‐A16, poly(I:C), siRNA...
Idiopathic inflammatory myopathies (IIM) are characterized by chronic inflammation, endothelial dysfunction, and muscle tissue mitochondrial defect, leading to the local oxidative stress response. However, data on its systemic intensity correlation with IIM clinical laboratory characteristics remains scarce. In clinically stable dermatomyositis (n = 18) myositis 38) patients matched controls 50), we measured global response in peripheral blood using a novel coumarin boronic acid (CBA) assay...
Hemocompatibility test of medical materials aims to detect adverse interaction between artificial surface and blood, which can activate or destruct blood components.In arterial flow conditions, due a high shear stress, platelet is the cell critical for hemocompatibility compliance.A classical instrumentation dynamic involves chamber with contact stream tested plate.In current study we investigated simplified model whole based on cone plate rotational viscometer.Several indices activation...
The role of miRNAs in the pathogenesis and determining phenotypes asthma is not fully elucidated. miR-146a has been previously shown to suppress inflammatory responses different cells. In this study, we investigated functions human bronchial epithelial cells (HBECs) association with neutrophilic, eosinophilic, paucigranulocytic asthma.Bronchial brushing specimens brochial mucosal biopsy samples were collected from adult patients age- gender-matched non-asthmatic individuals. expression...
<h3>Objectives</h3> Approximately 15% of adequately treated patients with early syphilis remain serofast. Pathogenesis and clinical significance this phenomenon is unclear. The objective study was to determine whether there any association between host immune response treatment outcome (serofast state or proper serological response). <h3>Methods</h3> Forty-four secondary were enrolled study. Levels pro-inflammatory cytokines such as interferon-γ, tumour necrosis factor-α interleukin-6...
Airway remodeling in asthma is characterized by reticular basement membrane (RBM) thickening, likely related to epithelial structural and functional changes. Gene expression profiling of the airway epithelium might identify genes involved bronchial alterations. We analyzed wall geometry (computed tomography (CT)), RBM thickness (histology), transcriptome profile (gene array) moderate severe persistent (n = 21) vs. no 19) airflow limitation asthmatics. was similar two studied subgroups. Among...
Airway inflammation in asthma is related to increased reactive oxygen species generation, potentially leading tissue injury and subsequent airway remodeling. We evaluated oxidative stress peripheral blood from asthmatic subjects (n = 74) matched controls 65), using recently developed real-time monitoring of the protein hydroperoxide (HP) formation by coumarin boronic acid (CBA) assay. also investigated relation systemic response disease severity, lung function, remodeling indices (lung...