A5024520846- Venous Thromboembolism Diagnosis and Management
- Blood properties and coagulation
- Atrial Fibrillation Management and Outcomes
- Blood Coagulation and Thrombosis Mechanisms
- Acute Ischemic Stroke Management
- Acute Myocardial Infarction Research
- Antiplatelet Therapy and Cardiovascular Diseases
- Platelet Disorders and Treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Lipoproteins and Cardiovascular Health
- Systemic Lupus Erythematosus Research
- Cardiac Valve Diseases and Treatments
- Cardiovascular Function and Risk Factors
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiac Arrhythmias and Treatments
- Asthma and respiratory diseases
- Vasculitis and related conditions
- Vitamin K Research Studies
- S100 Proteins and Annexins
- Cardiac and Coronary Surgery Techniques
- Atherosclerosis and Cardiovascular Diseases
- Eosinophilic Disorders and Syndromes
- Inflammatory Biomarkers in Disease Prognosis
Jagiellonian University
2016-2025
Institute of Cardiology
2016-2025
John Paul II Hospital
2016-2025
UCLouvain
2023
Krakow Cardiovascular Research Institute
2016
Cracow University of Technology
2016
Karolinska Institutet
2013
Abstract Background Accumulation of advanced glycation end products (AGEs) leads to chronic proteins and tissue damage, particularly in patients with diabetes mellitus (DM). We aimed evaluate whether increased accumulation AGEs aortic stenosis (AS) concomitant type 2 (DM) is associated AS severity. Methods prospectively enrolled 76 severe (47.1% males; nonDM), aged 68 [66–72] years, 50 age-matched DM a median blood glucose level 7.5 [5.9–9.1] mM glycated hemoglobin (HbA1c) 6.8 [6.3–7.8]%,...
Pulmonary embolism (PE) is a life-threatening manifestation of venous thromboembolism with high recurrence rate after anticoagulation cessation. Recently, we have reported that prothrombotic clot phenotype in patients associated an increased risk recurrent deep-vein thrombosis.We tested whether abnormal properties are predictive PE. We investigated 156 consecutive white aged 18 to 65 years the first-ever provoked or unprovoked PE (n=89), without thrombosis. Plasma fibrin permeability (Ks),...
Summary Background There is evidence that altered blood coagulation and fibrinolysis are involved in the pathogenesis of asthma. Increased thromboembolic risk has been reported asthmatics. Objective To investigate whether enhanced thrombin generation impaired occur Methods Plasma profile together with a computational assessment dynamics fibrinolytic capacity expressed as clot lysis time ( CLT ) were determined 164 consecutive patients stable asthma 72 controls matched for age, gender, weight...
Abstract Background Direct oral anticoagulants (DOACs) cause false positive lupus anticoagulant (LA) results. We assessed the impact of DOAC-Stop, reversing in vitro effects DOACs, on LA testing anticoagulated patients. Methods 75 venous thromboembolism patients aged 44.5±14.6 years. Blood samples were collected 2–28 h since intake including 50 rivaroxaban, 20 dabigatran and five apixaban. was performed at baseline after DOAC-Stop treatment. Positive defined as normalized (patient/standard...
Abstract Venous thromboembolism is associated with formation of denser fibrin clots resistant to lysis. We investigated whether prothrombotic plasma clot properties are the severity acute pulmonary embolism (PE). enrolled 126 normotensive PE patients (aged 58 ± 14 years) and 25 age- sex-matched healthy controls. Plasma permeability (K s ), lysis time (CLT), endogenous thrombin potential (ETP), plasminogen activator inhibitor-1 (PAI-1), citrullinated histone H3 (citH3) were evaluated on...
Atrial fibrillation (AF) is associated with cardiac remodelling and prothrombotic state. Enhanced neutrophil extracellular traps (NETs) formation has been reported in AF, contributing to thromboembolism.We investigated whether increased left atrium (LA) diameter reduced ventricular ejection fraction (LVEF) affect NETs state AF patients.In 243 patients (median CHA2 DS2 -VASc = 4) we measured LA LVEF, 123 of them LVEF<50%. Moreover, determined 3 markers NETosis: circulating citrullinated...
Microclots, a term also used for amyloid fibrin(ogen) particles and henceforth named aggregates, have recently been reported in the plasma of patients with COVID-19 long COVID. These aggregates implicated thrombotic complications these diseases.Plasma samples from 35 acute pulmonary embolism were collected analysed by laser scanning confocal microscopy electron before after clotting.Here we confirm presence show that they occur embolism, both clotting. Aggregates vary size consist fibrin...
Abstract Introduction Plasma protein carbonylation that reflects oxidative stress has been demonstrated to be associated with the prothrombotic fibrin clot phenotype. However, role of carbonyls (PC) in predicting ischemic stroke atrial fibrillation (AF) is largely unknown. This study aimed investigate whether PC increase risk anticoagulated AF patients during follow-up. Methods In 243 on anticoagulation (median age 69 years; median CHA 2 DS -VASc 4), we measured plasma using assay by...
Background Asthma is associated with a prothrombotic state. Plasma factor VIIa–antithrombin complex (FVIIa-AT) concentrations indirectly reflect the interaction of tissue (TF) FVII. Since TF key initiator coagulation in vivo, we hypothesized that FVIIa-AT are higher asthma. Methods In 159 clinically stable adult asthma patients and 62 controls, determined plasma analyzed their relation to circulating inflammatory markers together total potential for fibrinolysis (clot lysis time, CLT)...
Abstract Background The role of a prothrombotic state in atrial fibrillation (AF) progression to permanent arrythmia (PerAF) is unclear. Formation denser and poorly lysable fibrin clots has been observed AF patients also with sinus rhythm association higher stroke risk. We investigated whether altered clot properties other markers may contribute transition PerAF. Methods In the cohort study, 226 anticoagulated (median age 69 years, median CHA 2 DS ‐VASc 3) paroxysmal ( n = 83, 36.7%) or...
Abstract Introduction Protein carbonyl (PC) content, a stable marker of oxidative stress, is increased in chronic obstructive pulmonary disease (COPD) and shows association with cardiovascular events. We investigated prothrombotic effects PC content its modulation by statin use COPD. Materials Methods studied 56 patients COPD, who were randomly assigned an open‐label manner to receive simvastatin 40 mg/day ( n = 28) or remain without for 3 months 28). Plasma levels, along thrombin...
Atrial fibrillation (AF) coexists with left ventricular (LV) dysfunction and remodeling. Neutrophil infiltration of cardiac muscle tissue oxidative stress are associated In 221 AF patients aged 70 (62-76) years, LV remodeling was assessed using echocardiography. Plasma concentrations neutrophil-activating peptide 2 (NAP-2) 3-nitrotyrosine were measured. concentric eccentric hypertrophy showed higher NAP-2 levels than those normal geometry (+41% + 29%, p = 0.001 0.025, respectively)....
Abstract Background Diabetes mellitus (DM) has been shown to increase the rate of aortic stenosis (AS) progression. However, impact impaired plasma glucose on valvular calcification remains poorly understood. Using ex vivo micro-computed tomography (micro-CT), we aimed determine whether glucose, glycated haemoglobin (HbA 1c ), or concentrations advanced glycation end products (AGEs) and their soluble receptor (sRAGE) are associated with a specific pattern in severe AS. Methods In this...
Atrial fibrillation (AF), a risk factor for stroke and systemic thromboembolism, is associated with unfavorable fibrin clot properties increased thrombus formation in peripheral blood. The left atrial appendage (LAA) known to be the primary site of formation.We investigated relative differences plasma features including permeability (Ks) lysis time (CLT) between right atrium (RA), ventricle (RV), (LA), (LV), LAA, blood.Sixteen patients nonvalvular AF who stopped oral anticoagulant therapy at...
Determinants of intracoronary thrombus (ICT) composition in patients with ST-elevation myocardial infarction (STEMI) are largely unknown. We sought to investigate whether plasma fibrin phenotype and platelet reactivity affect ICT ultrastructure. assessed the content fibrin, platelets erythrocytes including polyhedrocytes by scanning electron microscopy on surface inside aspirated from 80 STEMI within 12 hours since chest pain onset. Plasma clot permeability (Ks), which indicates average pore...
Recently, we have reported that asthma is associated with enhanced plasma thrombin formation and impaired fibrinolysis. The mechanisms underlying the prothrombotic state in this disease are unknown. Our aim was to investigate whether alterations asthmatics inflammation. We studied 164 adult, white, stable 72 controls matched for age, sex, body mass index (BMI), smoking. Plasma tumor necrosis factor α (TNFα), interleukin (IL)-6, serum periostin were evaluated using ELISAs, their associations...