A5021312433- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Photoreceptor and optogenetics research
- Skin Protection and Aging
- Circadian rhythm and melatonin
- melanin and skin pigmentation
- Biochemical effects in animals
- Antioxidant Activity and Oxidative Stress
- Redox biology and oxidative stress
- Photodynamic Therapy Research Studies
- bioluminescence and chemiluminescence research
- Sirtuins and Resveratrol in Medicine
- Cell Adhesion Molecules Research
- Inflammatory Myopathies and Dermatomyositis
- Muscle Physiology and Disorders
- Cellular Mechanics and Interactions
- Sulfur Compounds in Biology
- Analytical Chemistry and Sensors
- Mast cells and histamine
- RNA Interference and Gene Delivery
- Hemoglobin structure and function
- Force Microscopy Techniques and Applications
- Connexins and lens biology
- Muscle and Compartmental Disorders
- Medical and Biological Ozone Research
Jagiellonian University
2015-2025
Medical College of Wisconsin
2022
Idiopathic inflammatory myopathies (IIM) are characterized by chronic inflammation, endothelial dysfunction, and muscle tissue mitochondrial defect, leading to the local oxidative stress response. However, data on its systemic intensity correlation with IIM clinical laboratory characteristics remains scarce. In clinically stable dermatomyositis (n = 18) myositis 38) patients matched controls 50), we measured global response in peripheral blood using a novel coumarin boronic acid (CBA) assay...
Airway inflammation in asthma is related to increased reactive oxygen species generation, potentially leading tissue injury and subsequent airway remodeling. We evaluated oxidative stress peripheral blood from asthmatic subjects (n = 74) matched controls 65), using recently developed real-time monitoring of the protein hydroperoxide (HP) formation by coumarin boronic acid (CBA) assay. also investigated relation systemic response disease severity, lung function, remodeling indices (lung...
Abstract Aging may significantly modify antioxidant and photoprotective properties of melanin in retinal pigment epithelium (RPE). Here, photoreactivity melanosomes (MS), isolated from younger older human donors with without added zeaxanthin α‐tocopherol, was analyzed by electron paramagnetic resonance oximetry, time‐resolved singlet oxygen phosphorescence, protein oxidation assay. The phototoxic potential ingested examined ARPE‐19 cells exposed to blue light. Phagocytosis FITC‐labeled...
Based on hitherto measurements of elasticity various cells in vitro and ex vivo, cancer are generally believed to be much softer than their normal counterparts. In spite significant research efforts the cells, only few studies were undertaken with melanoma cells. However, there no reports concerning pigmented Here, we report for first time human The obtained data show that melanin significantly increases stiffness effect depends amount inside dramatic impact nanomechanical properties puts...
The risk of chronic oxidative stress in the retinal pigment epithelium (RPE) increases with age due to accumulation photoreactive lipofuscin (LFG). Here, we asked whether sublethal and weakly lethal photic stress, induced by irradiation ARPE-19 cells containing phagocytised LFG, affected cell specific phagocytic activity, which is critically important for proper functioning survival retina, if natural antioxidants could modify observed outcomes. preloaded LFG isolated from human donors...
To determine whether previously shown photodynamic (PD)-induced inhibition of specific photoreceptor outer segment (POS) phagocytosis by ARPE-19 cells is associated with reductions in receptor proteins mediating POS phagocytosis, and if PD treatment merocyanine-540 (MC-540) produces additional effects leading to its nonspecific phagocytosis.ARPE-19 preloaded MC-540 or rose bengal (RB) were sublethally irradiated green light. Phagocytosis was measured flow cytometry (Mer tyrosine kinase...
Oxidative stress is a main factor responsible for key changes leading to the onset of age-related macular degeneration (ARMD) that occur in retinal pigment epithelium (RPE), which involved phagocytosis photoreceptor outer segments (POS). In this study, hydrogen peroxide (H2O2), H2O2 and iron ions (Fe) or rose Bengal (RB) presence NADH Fe were used model free radical mediated oxidative test if radicals singlet oxygen have different efficiency inhibit ARPE-19 cells. Free was confirmed by...
Curcumin is a plant-derived yellow-orange compound widely used as spice, dye and food additive. It also believed to have therapeutic effects against different disorders. On the other hand, there are data showing its phototoxicity bacteria, fungi various mammalian cells. Since mechanism of phototoxic action not fully understood, we investigated here potential curcumin in liposomal model membranes HaCaT First, detection singlet oxygen (1 O2 ) luminescence proved that generates 1 upon blue...
Abstract Efficient phagocytosis of photoreceptor outer segments (POS) membranes by retinal pigment epithelium (RPE) plays a key role in biological renewal these highly peroxidizable structures. Here, we tested whether photodynamic treatment, mediated merocyanine 540 (MC 540), rose Bengal or zinc‐substituted chlorophyllide inhibited phagocytic activity ARPE‐19 cells vitro. Specific fluorescein‐5‐isothiocyanate‐labeled POS isolated from cow retinas and nonspecific fluorescent polystyrene beads...
Although the primary biological function of retinal photoreceptors is to absorb light and provide visual information, extensive exposure intense could increase risk phototoxic reactions mediated by products rhodopsin bleaching that might accumulate in photoreceptor outer segments (POS). The phototoxicity POS, isolated from bovine retinas, was examined cultured pigment epithelium cells (ARPE-19) containing phagocytised POS selected model systems determining ability photogenerate singlet...
One of the most prominent age-related changes retinal pigment epithelium (RPE) is accumulation melanolipofuscin granules, which could contribute to oxidative stress in retina. The purpose this study was determine ability granules from younger and older donors photogenerate reactive oxygen species, examine if natural antioxidants modify phototoxic potential age pigment. Electron paramagnetic resonance (EPR) oximetry, EPR-spin trapping, time-resolved detection near-infrared phosphorescence...
Oxidative stress and inflammation in the retinal pigment epithelium (RPE) cells have been identified as significant risk factors development progression of associated diseases including age-related macular degeneration (AMD). AMD myopia also with impaired dopamine activity. RPE treated antioxidants slows high concentrations L-DOPA (levodopa) down-regulate vascular endothelial growth factor (VEGF) via GPR143, a G-protein-coupled receptor. To develop targeted effective treatment aimed at...
Phospholipids, representing 80% of retinal lipids, contain a high level polyunsaturated fatty acids, which are susceptible to oxidation. It has recently been shown that Folch’ extract bovine neural retinas reveals moderate photoreactivity by generating reactive oxygen species upon irradiation with blue light. Importantly, the markedly increases oxidation exctracted lipids. In this work, phototoxic potential (BRex), subjected controlled oxidation, is studied in vitro employing pigment...
Melanopsin, a member of the G protein-coupled receptors family, is involved in non-image-forming functions including circadian rhythm, sleep regulation and pupil response. In spite significant research efforts, signaling cascade involving melanopsin photoactivation remains poorly characterized. Here, we analyzed effects on phospholipase C (PLC) diacylglycerol. As an vitro model, HEK293 cells with stable expression human were used. Although both PLCβ1 PLCβ4 subtypes activated by cell exposure...
Chronic exposure of the retina to short wavelength visible light is a risk factor in pathogenesis age-related macular degeneration. The proper functioning and survival photoreceptors depends on efficient phagocytosis photoreceptor outer segments (POS) by retinal pigment epithelium. purpose this study was analyze phagocytic activity blue light-treated ARPE-19 cells, examine whether observed effects could be related altered levels POS receptor proteins and/or oxidation cellular lipids....